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You searched for: Journal FEMS microbiology letters Remove constraint Journal: FEMS microbiology letters Publication Year 2007 Remove constraint Publication Year: 2007 Subject Escherichia coli Remove constraint Subject: Escherichia coli
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1. Autoinducer-2 of the fire blight pathogen Erwinia amylovora and other plant-associated bacteria

Author:
Mohammadi, Mojtaba; Geider, Klaus
Source:
FEMS microbiology letters 2007 v.266 no.1 pp. 34-41
ISSN:
0378-1097
Subject:
DNA; Erwinia amylovora; Erwinia pyrifoliae; Escherichia coli; Pantoea stewartii; Pectobacterium carotovorum subsp. atrosepticum; Pseudomonas syringae; Salmonella Typhimurium; Vibrio harveyi; bacteria; genes; mutants; pathogens; pathovars; polymerase chain reaction
Abstract:
... Autoinducers are important for cellular communication of bacteria. The luxS gene has a central role in the synthesis of autoinducer-2 (AI-2). The gene was identified in a shotgun library of Erwinia amylovora and primers designed for PCR amplification from bacterial DNA. Supernatants of several Erwinia amylovora strains were assayed for AI-2 activity with a Vibrio harveyi mutant and were positive. ...
DOI:
10.1111/j.1574-6968.2006.00510.x
PubMed:
17092294

2. The growth advantage in stationary-phase (GASP) phenomenon in mixed cultures of enterobacteria

Author:
Bacun-Druzina, Visnja; Cagalj, Zeljka; Gjuracic, Kresimir
Source:
FEMS microbiology letters 2007 v.266 no.1 pp. 119-127
ISSN:
0378-1097
Subject:
Escherichia coli; Salmonella enterica; antibiotic resistance; chromosome aberrations; mixed culture; mutants; nalidixic acid; phenotype; streptomycin
Abstract:
... Growth advantage in stationary phase (GASP) is the term used to describe the ability of mutants with an increased fitness from 10-day-old enterobacteria culture to outcompete 1-day-old cells of the same initial strain during a prolonged stationary phase, although the aged cells are introduced as a minority. We studied this bacterial trait in mixed cultures of two enterobacterial species, Escherich ...
DOI:
10.1111/j.1574-6968.2006.00515.x
PubMed:
17233722

3. Identification of a Moraxella catarrhalis gene that confers adherence to various human epithelial cell lines in vitro

Author:
Lipski, Serena L.; Holm, Melissa M.; Lafontaine, Eric R.
Source:
FEMS microbiology letters 2007 v.267 no.2 pp. 207-213
ISSN:
0378-1097
Subject:
Escherichia coli; Moraxella catarrhalis; bacteria; epithelial cells; genes; humans; larynx; pathogenesis; pathogens
Abstract:
... Moraxella catarrhalis is a pathogen of the human airways. We found that expression of the M. catarrhalis gene mcmA by Escherichia coli increases adherence to epithelial cells 100-fold. Furthermore, we discovered that disrupting mcmA decreases M. catarrhalis adherence to laryngeal and lung cells, which are relevant to pathogenesis by the bacterium. ...
DOI:
10.1111/j.1574-6968.2006.00549.x
PubMed:
17166229

4. Mutational analysis of the zinc metalloprotease EmpA of Vibrio anguillarum

Author:
Yang, Hui; Chen, Jixiang; Yang, Guanpin; Zhang, Xiao-Hua; Li, Yun
Source:
FEMS microbiology letters 2007 v.267 no.1 pp. 56-63
ISSN:
0378-1097
Subject:
Escherichia coli; Vibrio anguillarum; active sites; cytotoxicity; enzyme activity; flounder; genes; metalloproteinases; mutants; pathogenesis; pathogens; proteolysis; site-directed mutagenesis; virulence; zinc
Abstract:
... The extracellular zinc metalloprotease, EmpA, is a putative virulence factor involved in pathogenicity of the fish pathogen Vibrio anguillarum. The 611-amino acid precursor of this enzyme is encoded by the empA gene. The residues His³⁴⁶, His³⁵⁰, Glu³⁷⁰, Glu³⁴⁷, His⁴²⁹, Tyr³⁶¹ and Asp⁴¹⁷ are highly conserved and putatively function together at the active site of the enzyme. In this study, empA was ...
DOI:
10.1111/j.1574-6968.2006.00533.x

5. The role of the omega subunit of RNA polymerase in expression of the relA gene in Escherichia coli

Author:
Chatterji, Dipankar; Ogawa, Yoshito; Shimada, Tomohiro; Ishihama, Akira
Source:
FEMS microbiology letters 2007 v.267 no.1 pp. 51-55
ISSN:
0378-1097
Subject:
messenger RNA; starvation; stress response; DNA microarrays; gene expression; ribosomal proteins; operon; DNA-directed RNA polymerase; Escherichia coli
Abstract:
... The rpoZ gene for the omega subunit of Escherichia coli RNA polymerase constitutes single operon with the spoT gene, which is responsible for the maintenance of stringent response under nutrient starvation conditions. To identify the physiological role of the omega subunit, we compared the gene expression profile of wild-type Escherichia coli with that of an rpoZ deleted strain by microarray analy ...
DOI:
10.1111/j.1574-6968.2006.00532.x

6. Characterization of Treponema denticola pyrF encoding orotidine-5'-monophosphate decarboxylase

Author:
Capone, Ricardo F.; Ning, Yu; Pakulis, Nora; Alhazzazi, Turki; Christopher Fenno, J.
Source:
FEMS microbiology letters 2007 v.268 no.2 pp. 261-267
ISSN:
0378-1097
Subject:
Escherichia coli; Treponema denticola; biosynthesis; genes; mutants; orotidine-5'-phosphate decarboxylase; prediction; uracil
Abstract:
... The Treponema denticola ATCC 35405 genome annotation contains most of the genes for de novo pyrimidine biosynthesis. To initiate characterization of pyrimidine synthesis in Treponema, we focused on TDE2110 (the putative pyrF, encoding orotidine-5'-monophosphate decarboxlyase). Unlike the parent strain, an isogenic pyrF mutant was resistant to 5-fluoroorotic acid. In complex medium, growth of the p ...
DOI:
10.1111/j.1574-6968.2006.00589.x
PubMed:
17187656

7. Transcription in Escherichia coli PHL628 biofilms

Author:
Junker, Lauren M.; Toba, F. Andreas; Hay, Anthony G.
Source:
FEMS microbiology letters 2007 v.268 no.2 pp. 237-243
ISSN:
0378-1097
Subject:
Escherichia coli; biofilm; carbon; gene expression; gene induction; genes; mutants; nutrients; transcription (genetics)
Abstract:
... The biofilm-specific gene expression of Escherichia coli PHL628 was compared with that from exponentially growing planktonic cells using macroarray technology. In duplicate experiments, both biofilm and planktonic cells were grown in separate continually stirred tank reactors at 57% of the maximal planktonic growth rate. When transcriptional results from planktonic cultures were compared with that ...
DOI:
10.1111/j.1574-6968.2006.00585.x
PubMed:
17227468

8. Two noncellulosomal cellulases of Clostridium thermocellum, Cel9I and Cel48Y, hydrolyse crystalline cellulose synergistically

Author:
Berger, Emanuel; Zhang, Dong; Zverlov, Vladimir V.; Schwarz, Wolfgang H.
Source:
FEMS microbiology letters 2007 v.268 no.2 pp. 194-201
ISSN:
0378-1097
Subject:
Clostridium thermocellum; barley; xylanases; cellulosome; chitinase; carboxymethylcellulose; genes; hydrolysis; amylases; synergism; endo-1,4-beta-glucanase; beta-glucans; Escherichia coli
Abstract:
... The genome of Clostridium thermocellum contains a number of genes for polysaccharide degradation-associated proteins that are not cellulosome bound. The list includes β-glucanases, glycosidases, chitinases, amylases and a xylanase. One of these 'soluble'-enzyme genes codes for a second glycosyl hydrolase (GH)48 cellulase, Cel48Y, which was expressed in Escherichia coli and biochemically characteri ...
DOI:
10.1111/j.1574-6968.2006.00583.x

9. Resistance phenotypes conferred by macrolide phosphotransferases

Author:
Chesneau, Olivier; Tsvetkova, Krassimira; Courvalin, Patrice
Source:
FEMS microbiology letters 2007 v.269 no.2 pp. 317-322
ISSN:
0378-1097
Subject:
stop codon; azithromycin; phenotype; antibiotic resistance; spiramycin; telithromycin; phosphotransferases (kinases); phosphorylation; Escherichia coli
Abstract:
... This study aims to compare the resistance phenotypes conferred by various genes encoding enzymes that phosphorylate erythromycin. The mph genes were cloned into Escherichia coli AG100A susceptible to macrolides and ketolides following disruption of the AcrAB pump. An 882 bp sequence containing a premature stop codon, homologous to the three other previously described mph genes and present widely a ...
DOI:
10.1111/j.1574-6968.2007.00643.x
PubMed:
17302923

10. The Hek outer membrane protein of Escherichia coli is an auto-aggregating adhesin and invasin

Author:
Fagan, Robert P.; Smith, Stephen G.J.
Source:
FEMS microbiology letters 2007 v.269 no.2 pp. 248-255
ISSN:
0378-1097
Subject:
meningitis; gastrointestinal system; adhesins; epithelial cells; meninges; physiological transport; blood flow; erythrocytes; neonates; pathogenesis; adhesion; agglutination; sepsis (infection); Escherichia coli
Abstract:
... Escherichia coli is the principal gram-negative causative agent of sepsis and meningitis in neonates. The pathogenesis of meningitis due to E. coli K1 involves mucosal colonization, transcytosis of epithelial cells, survival in the blood stream and eventually invasion of the meninges. The latter two aspects have been well characterized at a molecular level in the last decade. Less is known about t ...
DOI:
10.1111/j.1574-6968.2006.00628.x
PubMed:
17241243

11. The Xanthomonas oryzae pv. oryzae eglXoB endoglucanase gene is required for virulence to rice

Author:
Hu, Jun; Qian, Wei; He, Chaozu
Source:
FEMS microbiology letters 2007 v.269 no.2 pp. 273-279
ISSN:
0378-1097
Subject:
Escherichia coli; Oryza sativa; Xanthomonas oryzae pv. oryzae; cellulose; cultivars; endo-1,4-beta-glucanase; genes; genetic complementation; leaf blight; leaves; mutants; pathogenesis; reverse transcriptase polymerase chain reaction; rice; screening; sugars; transposons; virulence
Abstract:
... Xanthomonas oryzae pv. oryzae (Xoo) is the causal agent of bacterial leaf blight, a serious disease of rice worldwide. A Tn5-based transposon randomly insertional mutant library was previously constructed. By screening mutants against susceptible rice cultivar IR24, four mutants were identified with reduced virulence on rice plants and were found to have Tn5 transposon inserted at an endo-1,4-β- d ...
DOI:
10.1111/j.1574-6968.2007.00638.x
PubMed:
17326805

12. ampC gene expression in promoter mutants of cefoxitin-resistant Escherichia coli clinical isolates

Author:
Tracz, Dobryan M.; Boyd, David A.; Hizon, Romeo; Bryce, Elizabeth; McGeer, Allison; Ofner-Agostini, Marianna; Simor, Andrew E.; Paton, Shirley; Mulvey, Michael R.
Source:
FEMS microbiology letters 2007 v.270 no.2 pp. 265-271
ISSN:
0378-1097
Subject:
Escherichia coli; cefoxitin; gene overexpression; genes; minimum inhibitory concentration; mutants; mutation; promoter regions; reverse transcriptase polymerase chain reaction; transposons
Abstract:
... Reverse transcriptase polymerase chain reaction was used to determine the amount of overexpression of the ampC gene in 52 cefoxitin-resistant Escherichia coli clinical isolates that had previously characterized mutations in their ampC promoter/attenuator regions. The results showed that mutations that create a consensus -35 box (TTGACA) are the most important factor in strengthening the ampC promo ...
DOI:
10.1111/j.1574-6968.2007.00672.x
PubMed:
17326753

13. Efficient silencing of the gene coding for the ε subunit of DNA polymerase III in Escherichia coli is triggered by antisense RNAs featuring stability in vivo

Author:
Stefan, Alessandra; Tabler, Martin; Hochkoeppler, Alejandro
Source:
FEMS microbiology letters 2007 v.270 no.2 pp. 277-283
ISSN:
0378-1097
Subject:
DNA-directed DNA polymerase; Escherichia coli; gene silencing; genes; messenger RNA
Abstract:
... The Escherichia coli gene dnaQ coding for the ε subunit of DNA polymerase III was suppressed in vivo via antisense RNAs. To this aim, different fragments of dnaQ were cloned in reverse orientation into the pBAD-HisB vector or into pT3T7, and the corresponding antisense RNAs were conditionally expressed in vivo. Antisense transcripts featuring fast hybridization in vitro with dnaQ mRNA but lacking ...
DOI:
10.1111/j.1574-6968.2007.00679.x

14. Mechanisms of action of ostrich β-defensins against Escherichia coli

Author:
Sugiarto, Haryadi; Yu, Pak-Lam
Source:
FEMS microbiology letters 2007 v.270 no.2 pp. 195-200
ISSN:
0378-1097
Subject:
DNA; Escherichia coli; Gram-negative bacteria; RNA; anti-infective properties; antimicrobial peptides; cell membranes; enzyme activity; humans; lipopolysaccharides; mechanism of action; microbial growth; neutrophils; ostriches; protein synthesis; sheep
Abstract:
... To understand their mechanism of antimicrobial activity against Gram-negative bacteria, ostrich β-defensins, ostricacins-1 and 2 (Osp-1 and Osp-2), were compared with those of sheep myeloid antimicrobial peptide (SMAP)-29 and human neutrophil peptide (HNP)-1, well-characterized sheep α-helical and human α-defensin peptides, respectively. Fluorescence-based biochemical assays demonstrated that the ...
DOI:
10.1111/j.1574-6968.2007.00642.x

15. MmcBC in Pelotomaculum thermopropionicum represents a novel group of prokaryotic fumarases

Author:
Shimoyama, Takefumi; Rajashekhara, Eranna; Ohmori, Daijiro; Kosaka, Tomoyuki; Watanabe, Kazuya
Source:
FEMS microbiology letters 2007 v.270 no.2 pp. 207-213
ISSN:
0378-1097
Subject:
Escherichia coli; amino acid sequences; electron paramagnetic resonance spectroscopy; molecular weight; temperature
Abstract:
... The overall amino-acid sequence of MmcBC in Pelotomaculum thermopropionicum was substantially homologous (33%) to fumarase A in Escherichia coli, although its possible subunit structure was different from known fumarases and it lacked the fumarate-lyase signature sequence. Here, MmcBC in E. coli is expressed and characterized. The purified enzyme catalyzed reversible conversion of fumarate to l-ma ...
DOI:
10.1111/j.1574-6968.2007.00665.x

16. Phosphorylation of Type IA restriction-modification complex enzyme EcoKI on the HsdR subunit

Author:
Cajthamlová, Kamila; Šišáková, Eva; Weiser, Jaroslav; Weiserová, Marie
Source:
FEMS microbiology letters 2007 v.270 no.1 pp. 171-177
ISSN:
0378-1097
Subject:
Escherichia coli; genes; immunoblotting; phosphoproteins; phosphorylation; restriction endonucleases; subcellular fractions
Abstract:
... Phosphorylation of Type I restriction-modification (R-M) enzymes EcoKI, EcoAI, and EcoR124I - representatives of IA, IB, and IC families, respectively - was analysed in vivo by immunoblotting of endogenous phosphoproteins isolated from Escherichia coli strains harbouring the corresponding hsd genes, and in vitro by a phosphorylation assay using protein kinase present in subcellular fractions of E. ...
DOI:
10.1111/j.1574-6968.2007.00663.x
PubMed:
17439637

17. The Escherichia coli protein YjjG is a house-cleaning nucleotidase in vivo

Author:
Titz, Björn; Häuser, Roman; Engelbrecher, Anne; Uetz, Peter
Source:
FEMS microbiology letters 2007 v.270 no.1 pp. 49-57
ISSN:
0378-1097
Subject:
DNA; Escherichia coli; adverse effects; drug therapy; enzyme activity; fluorouracil; humans; mutagenicity; neoplasms; nucleotidase; protective effect; thymidine monophosphate
Abstract:
... House-cleaning enzymes protect cells from the adverse effects of noncanonical metabolic chemical compounds. The Escherichia coli nucleotide phosphatase YjjG (B4374, JW4336) functions as a house-cleaning phosphatase in vivo. YjjG protects the cell against noncanonical pyrimidine derivatives such as 5-fluoro-2'-deoxyuridine (5-FdUridine), 5-fluorouridine, 5-fluoroorotic acid (5-FOA), 5-fluorouracil, ...
DOI:
10.1111/j.1574-6968.2007.00646.x
PubMed:
17286574

18. Acquisition and diffusion of blaCTX₋M₋₉ gene by R478-IncHI2 derivative plasmids

Author:
García, Aurora; Navarro, Ferran; Miró, Elisenda; Villa, Laura; Mirelis, Beatriz; Coll, Pere; Carattoli, Alessandra
Source:
FEMS microbiology letters 2007 v.271 no.1 pp. 71-77
ISSN:
0378-1097
Subject:
Escherichia coli; Salmonella enterica; genes; plasmids; Spain
Abstract:
... Escherichia coli and Salmonella enterica isolates carrying the blaCTX₋M₋₉ gene located on plasmids prevailed at the Hospital de la Santa Creu i Sant Pau, Barcelona, Spain in the 1996-1999 period. The blaCTX₋M₋₉-carrying plasmids showed a great variability in size, suggesting the mobilization of the gene among different plasmid scaffolds. The aim of the present work was to identify and better chara ...
DOI:
10.1111/j.1574-6968.2007.00695.x

19. A new role of Deinococcus radiodurans RecD in antioxidant pathway

Author:
Zhou, Qing; Zhang, Xinjue; Xu, Hong; Xu, Bujin; Hua, Yuejin
Source:
FEMS microbiology letters 2007 v.271 no.1 pp. 118-125
ISSN:
0378-1097
Subject:
Deinococcus radiodurans; Escherichia coli; catalase; enzyme activity; free radical scavengers; genes; hydrogen peroxide; phenotype; ultraviolet radiation
Abstract:
... In Deinococcus radiodurans, RecBCD holoenzyme is not intact because of the absence of RecB and RecC, but a RecD-like protein does indeed exist. In this work, D. radiodurans recD disruptant was constructed and its possible biological functions were investigated. The results showed that disruption of the recD gene of D. radiodurans resulted in a remarkably increased sensitivity to hydrogen peroxide ...
DOI:
10.1111/j.1574-6968.2007.00703.x
PubMed:
17419762

20. A novel gene with antisalt and anticadmium stress activities from a halotolerant marine green alga Chlamydomonas sp. W80

Author:
Tanaka, Satoshi; Suda, Yoshito; Ikeda, Kazunori; Ono, Masahiro; Miyasaka, Hitoshi; Watanabe, Masanori; Sasaki, Ken; Hirata, Kazumasa
Source:
FEMS microbiology letters 2007 v.271 no.1 pp. 48-52
ISSN:
0378-1097
Subject:
Chlamydomonas; Escherichia coli; aspartic acid; cDNA libraries; cadmium; complementary DNA; genes; histidine; metal tolerance; salt tolerance; screening; sodium chloride
Abstract:
... A novel gene with antistress activities against both salt (NaCl) and cadmium stresses was isolated from the cDNA library of halotolerant green alga Chlamydomonas sp. strain W80 by a functional expression screening with Escherichia coli. The C-terminal region of this protein is responsible for the antistress activity, because N-terminal truncated clone of this gene retains the antistress activity, ...
DOI:
10.1111/j.1574-6968.2007.00696.x
PubMed:
17391362