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- Tasara, Taurai; Stephan, Roger
- FEMS microbiology letters 2007 v.269 no.2 pp. 265-272
- DNA-directed RNA polymerase; Listeria monocytogenes; beta-glucosidase; cold; essential genes; food pathogens; gene expression; gene expression regulation; messenger RNA; models; quantitative polymerase chain reaction; reverse transcriptase polymerase chain reaction; ribosomal RNA; sodium chloride
- ... Listeria monocytogenes is an important food-borne pathogen that can tolerate a wide range of stress conditions. However, its stress adaptation processes are still poorly understood. Real-time-based quantitative RT-PCR (qRT-PCR) provides a tool to probe gene expression changes underlying stress adaptation. But, a limitation to study mRNA levels by real-time qRT-PCR is that validated reference genes ...
- Chatterji, Dipankar; Ogawa, Yoshito; Shimada, Tomohiro; Ishihama, Akira
- FEMS microbiology letters 2007 v.267 no.1 pp. 51-55
- messenger RNA; starvation; stress response; DNA microarrays; gene expression; ribosomal proteins; operon; DNA-directed RNA polymerase; Escherichia coli
- ... The rpoZ gene for the omega subunit of Escherichia coli RNA polymerase constitutes single operon with the spoT gene, which is responsible for the maintenance of stringent response under nutrient starvation conditions. To identify the physiological role of the omega subunit, we compared the gene expression profile of wild-type Escherichia coli with that of an rpoZ deleted strain by microarray analy ...