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- Author:
- Chatterji, Dipankar; Ogawa, Yoshito; Shimada, Tomohiro; Ishihama, Akira
- Source:
- FEMS microbiology letters 2007 v.267 no.1 pp. 51-55
- ISSN:
- 0378-1097
- Subject:
- messenger RNA; starvation; stress response; DNA microarrays; gene expression; ribosomal proteins; operon; DNA-directed RNA polymerase; Escherichia coli
- Abstract:
- ... The rpoZ gene for the omega subunit of Escherichia coli RNA polymerase constitutes single operon with the spoT gene, which is responsible for the maintenance of stringent response under nutrient starvation conditions. To identify the physiological role of the omega subunit, we compared the gene expression profile of wild-type Escherichia coli with that of an rpoZ deleted strain by microarray analy ...
- DOI:
- 10.1111/j.1574-6968.2006.00532.x
-
http://dx.doi.org/10.1111/j.1574-6968.2006.00532.x
- Author:
- Magnani, T.; Soriani, F.M.; Martins, V.P.; Nascimento, A.M.; Tudella, V.G.; Curti, C.; Uyemura, S.A.
- Source:
- FEMS microbiology letters 2007 v.271 no.2 pp. 230-238
- ISSN:
- 0378-1097
- Subject:
- Aspergillus fumigatus; Escherichia coli; Saccharomyces cerevisiae; electron transport chain; gene expression; genes; heterologous gene expression; menadione; messenger RNA; open reading frames; oxidative stress; oxygen; oxygen consumption; paraquat; potassium cyanide; reactive oxygen species
- Abstract:
- ... Aspergillus fumigatus possesses a branched mitochondrial electron transport chain, with both cyanide-sensitive and -insensitive oxygen-consumption activities. Mitochondrial reactive oxygen species mediate signaling for alternative oxidase (AOX) expression. A 1173 bp-long Afaox gene encoding a 40 kDa protein has been cloned and identified. Recombinant constructs containing the Afaox ORF were transf ...
- DOI:
- 10.1111/j.1574-6968.2007.00716.x
- PubMed:
- 17425662
-
http://dx.doi.org/10.1111/j.1574-6968.2007.00716.x
- Author:
- Huang, Yong; Zhang, Bin; Dong, Ke; Zhang, XiaoNan; Hou, LiChao; Wang, Tao; Chen, NanChun; Chen, SuMin
- Source:
- FEMS microbiology letters 2007 v.275 no.1 pp. 8-15
- ISSN:
- 0378-1097
- Subject:
- DNA; Escherichia coli; amino acid sequences; bacteriophages; gene expression; gene expression regulation; genes; guanosinetriphosphatase; heat; heat shock proteins; messenger RNA; metalloproteinases; microbial growth; mutants; stress response
- Abstract:
- ... Era is a highly conserved GTPase essential for bacterial growth. Using a digoxigenin-labeled Era protein to screen a phage expression library of Escherichia coli genomic DNA, yggG, a gene that encodes a putative zinc metalloprotease was isolated and characterized. The deduced amino acid sequence of YggG showed high degrees of similarity to some reported heat shock proteins. In this study, the dire ...
- DOI:
- 10.1111/j.1574-6968.2007.00860.x
- PubMed:
- 17651431
-
http://dx.doi.org/10.1111/j.1574-6968.2007.00860.x
- Author:
- Chouayekh, Hichem; Virolle, Marie-Joëlle
- Source:
- FEMS microbiology letters 2007 v.276 no.1 pp. 42-47
- ISSN:
- 0378-1097
- Subject:
- Escherichia coli; Streptomyces lividans; gene expression; genes; messenger RNA; nucleotides; ribonucleases; site-directed mutagenesis
- Abstract:
- ... In Streptomyces lividans, the tight temporal regulation of the transient expression of the sblA gene was shown to involve an operator-like sequence located on the sblA transcript. This operator-like structure constitutes a stem-loop structure containing a Shine/Dalgarno-like sequence. Its destruction, by site directed mutagenesis, led to an enhancement of sblA expression. This structure thus plays ...
- DOI:
- 10.1111/j.1574-6968.2007.00912.x
- PubMed:
- 17877728
-
http://dx.doi.org/10.1111/j.1574-6968.2007.00912.x