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- Author:
- Magnani, T.; Soriani, F.M.; Martins, V.P.; Nascimento, A.M.; Tudella, V.G.; Curti, C.; Uyemura, S.A.
- Source:
- FEMS microbiology letters 2007 v.271 no.2 pp. 230-238
- ISSN:
- 0378-1097
- Subject:
- Aspergillus fumigatus; Escherichia coli; Saccharomyces cerevisiae; electron transport chain; gene expression; genes; heterologous gene expression; menadione; messenger RNA; open reading frames; oxidative stress; oxygen; oxygen consumption; paraquat; potassium cyanide; reactive oxygen species
- Abstract:
- ... Aspergillus fumigatus possesses a branched mitochondrial electron transport chain, with both cyanide-sensitive and -insensitive oxygen-consumption activities. Mitochondrial reactive oxygen species mediate signaling for alternative oxidase (AOX) expression. A 1173 bp-long Afaox gene encoding a 40 kDa protein has been cloned and identified. Recombinant constructs containing the Afaox ORF were transf ...
- DOI:
- 10.1111/j.1574-6968.2007.00716.x
- PubMed:
- 17425662
-
http://dx.doi.org/10.1111/j.1574-6968.2007.00716.x
- Author:
- Stefan, Alessandra; Tabler, Martin; Hochkoeppler, Alejandro
- Source:
- FEMS microbiology letters 2007 v.270 no.2 pp. 277-283
- ISSN:
- 0378-1097
- Subject:
- DNA-directed DNA polymerase; Escherichia coli; gene silencing; genes; messenger RNA
- Abstract:
- ... The Escherichia coli gene dnaQ coding for the ε subunit of DNA polymerase III was suppressed in vivo via antisense RNAs. To this aim, different fragments of dnaQ were cloned in reverse orientation into the pBAD-HisB vector or into pT3T7, and the corresponding antisense RNAs were conditionally expressed in vivo. Antisense transcripts featuring fast hybridization in vitro with dnaQ mRNA but lacking ...
- DOI:
- 10.1111/j.1574-6968.2007.00679.x
-
http://dx.doi.org/10.1111/j.1574-6968.2007.00679.x
- Author:
- Dowd, Scot E.
- Source:
- FEMS microbiology letters 2007 v.273 no.2 pp. 214
- ISSN:
- 0378-1097
- Subject:
- Escherichia coli O157:H7; animal pathogenic bacteria; virulence; bacterial proteins; messenger RNA; gene expression regulation; iron; gastrointestinal system; catecholamines; norepinephrine; host-pathogen relationships; pathogenesis; Escherichia coli
- Abstract:
- ... Various forms of host stresses (e.g. physiological, psychological) are thought to influence susceptibility to pathogenic microorganisms. Catecholamines such as norepinephrine are released into the GI environment during acute stress and may influence the infective process of bacterial pathogens associated with the GI tract. To examine the effects of norepinephrine on expression of virulence factors ...
- Handle:
- 10113/2501
- DOI:
- 10.1111/j.1574-6968.2007.00800.x
- PubMed:
- 17573936
-
http://dx.doi.org/10.1111/j.1574-6968.2007.00800.x
- Author:
- Chouayekh, Hichem; Virolle, Marie-Joëlle
- Source:
- FEMS microbiology letters 2007 v.276 no.1 pp. 42-47
- ISSN:
- 0378-1097
- Subject:
- Escherichia coli; Streptomyces lividans; gene expression; genes; messenger RNA; nucleotides; ribonucleases; site-directed mutagenesis
- Abstract:
- ... In Streptomyces lividans, the tight temporal regulation of the transient expression of the sblA gene was shown to involve an operator-like sequence located on the sblA transcript. This operator-like structure constitutes a stem-loop structure containing a Shine/Dalgarno-like sequence. Its destruction, by site directed mutagenesis, led to an enhancement of sblA expression. This structure thus plays ...
- DOI:
- 10.1111/j.1574-6968.2007.00912.x
- PubMed:
- 17877728
-
http://dx.doi.org/10.1111/j.1574-6968.2007.00912.x
- Author:
- Chatterji, Dipankar; Ogawa, Yoshito; Shimada, Tomohiro; Ishihama, Akira
- Source:
- FEMS microbiology letters 2007 v.267 no.1 pp. 51-55
- ISSN:
- 0378-1097
- Subject:
- messenger RNA; starvation; stress response; DNA microarrays; gene expression; ribosomal proteins; operon; DNA-directed RNA polymerase; Escherichia coli
- Abstract:
- ... The rpoZ gene for the omega subunit of Escherichia coli RNA polymerase constitutes single operon with the spoT gene, which is responsible for the maintenance of stringent response under nutrient starvation conditions. To identify the physiological role of the omega subunit, we compared the gene expression profile of wild-type Escherichia coli with that of an rpoZ deleted strain by microarray analy ...
- DOI:
- 10.1111/j.1574-6968.2006.00532.x
-
http://dx.doi.org/10.1111/j.1574-6968.2006.00532.x
- Author:
- Huang, Yong; Zhang, Bin; Dong, Ke; Zhang, XiaoNan; Hou, LiChao; Wang, Tao; Chen, NanChun; Chen, SuMin
- Source:
- FEMS microbiology letters 2007 v.275 no.1 pp. 8-15
- ISSN:
- 0378-1097
- Subject:
- DNA; Escherichia coli; amino acid sequences; bacteriophages; gene expression; gene expression regulation; genes; guanosinetriphosphatase; heat; heat shock proteins; messenger RNA; metalloproteinases; microbial growth; mutants; stress response
- Abstract:
- ... Era is a highly conserved GTPase essential for bacterial growth. Using a digoxigenin-labeled Era protein to screen a phage expression library of Escherichia coli genomic DNA, yggG, a gene that encodes a putative zinc metalloprotease was isolated and characterized. The deduced amino acid sequence of YggG showed high degrees of similarity to some reported heat shock proteins. In this study, the dire ...
- DOI:
- 10.1111/j.1574-6968.2007.00860.x
- PubMed:
- 17651431
-
http://dx.doi.org/10.1111/j.1574-6968.2007.00860.x