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You searched for: Journal FEMS microbiology letters Remove constraint Journal: FEMS microbiology letters Subject messenger RNA Remove constraint Subject: messenger RNA Subject Escherichia coli Remove constraint Subject: Escherichia coli Subject gene expression Remove constraint Subject: gene expression
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1. The role of the omega subunit of RNA polymerase in expression of the relA gene in Escherichia coli

Author:
Chatterji, Dipankar; Ogawa, Yoshito; Shimada, Tomohiro; Ishihama, Akira
Source:
FEMS microbiology letters 2007 v.267 no.1 pp. 51-55
ISSN:
0378-1097
Subject:
messenger RNA; starvation; stress response; DNA microarrays; gene expression; ribosomal proteins; operon; DNA-directed RNA polymerase; Escherichia coli
Abstract:
... The rpoZ gene for the omega subunit of Escherichia coli RNA polymerase constitutes single operon with the spoT gene, which is responsible for the maintenance of stringent response under nutrient starvation conditions. To identify the physiological role of the omega subunit, we compared the gene expression profile of wild-type Escherichia coli with that of an rpoZ deleted strain by microarray analy ...
DOI:
10.1111/j.1574-6968.2006.00532.x

2. Cloning and functional expression of the mitochondrial alternative oxidase of Aspergillus fumigatus and its induction by oxidative stress

Author:
Magnani, T.; Soriani, F.M.; Martins, V.P.; Nascimento, A.M.; Tudella, V.G.; Curti, C.; Uyemura, S.A.
Source:
FEMS microbiology letters 2007 v.271 no.2 pp. 230-238
ISSN:
0378-1097
Subject:
Aspergillus fumigatus; Escherichia coli; Saccharomyces cerevisiae; electron transport chain; gene expression; genes; heterologous gene expression; menadione; messenger RNA; open reading frames; oxidative stress; oxygen; oxygen consumption; paraquat; potassium cyanide; reactive oxygen species
Abstract:
... Aspergillus fumigatus possesses a branched mitochondrial electron transport chain, with both cyanide-sensitive and -insensitive oxygen-consumption activities. Mitochondrial reactive oxygen species mediate signaling for alternative oxidase (AOX) expression. A 1173 bp-long Afaox gene encoding a 40 kDa protein has been cloned and identified. Recombinant constructs containing the Afaox ORF were transf ...
DOI:
10.1111/j.1574-6968.2007.00716.x
PubMed:
17425662

3. Up-regulation of yggG promotes the survival of Escherichia coli cells containing Era-1 mutant protein

Author:
Huang, Yong; Zhang, Bin; Dong, Ke; Zhang, XiaoNan; Hou, LiChao; Wang, Tao; Chen, NanChun; Chen, SuMin
Source:
FEMS microbiology letters 2007 v.275 no.1 pp. 8-15
ISSN:
0378-1097
Subject:
DNA; Escherichia coli; amino acid sequences; bacteriophages; gene expression; gene expression regulation; genes; guanosinetriphosphatase; heat; heat shock proteins; messenger RNA; metalloproteinases; microbial growth; mutants; stress response
Abstract:
... Era is a highly conserved GTPase essential for bacterial growth. Using a digoxigenin-labeled Era protein to screen a phage expression library of Escherichia coli genomic DNA, yggG, a gene that encodes a putative zinc metalloprotease was isolated and characterized. The deduced amino acid sequence of YggG showed high degrees of similarity to some reported heat shock proteins. In this study, the dire ...
DOI:
10.1111/j.1574-6968.2007.00860.x
PubMed:
17651431

4. Fate of the sblA transcript in Streptomyces lividans and Escherichia coli

Author:
Chouayekh, Hichem; Virolle, Marie-Joëlle
Source:
FEMS microbiology letters 2007 v.276 no.1 pp. 42-47
ISSN:
0378-1097
Subject:
Escherichia coli; Streptomyces lividans; gene expression; genes; messenger RNA; nucleotides; ribonucleases; site-directed mutagenesis
Abstract:
... In Streptomyces lividans, the tight temporal regulation of the transient expression of the sblA gene was shown to involve an operator-like sequence located on the sblA transcript. This operator-like structure constitutes a stem-loop structure containing a Shine/Dalgarno-like sequence. Its destruction, by site directed mutagenesis, led to an enhancement of sblA expression. This structure thus plays ...
DOI:
10.1111/j.1574-6968.2007.00912.x
PubMed:
17877728

5. Prevotella intermedia induces matrix metalloproteinase-9 expression in human periodontal ligament cells

Author:
Guan, Su-Min; Shu, Lei; Fu, Shan-Min; Liu, Bin; Xu, Xiu-Li; Wu, Jun-Zheng
Source:
FEMS microbiology letters 2008 v.283 no.1 pp. 47-53
ISSN:
0378-1097
Subject:
Escherichia coli; Prevotella intermedia; gelatin; gelatinase B; gene expression; humans; interleukin-1beta; ligaments; messenger RNA; mitogen-activated protein kinase; pathogens; periodontitis; protein secretion; reverse transcriptase polymerase chain reaction
Abstract:
... Matrix metalloproteinases (MMPs) play pivotal roles in inflammatory diseases including chronic periodontitis. The effects of Prevotella intermedia, a major periodontal pathogen, on MMP-9 production in primary human periodontal ligament (hPDL) cells were examined in the present study. MMP-9 mRNA expression was measured by semiquantitative reverse transcriptase PCR and its protein secretion was assa ...
DOI:
10.1111/j.1574-6968.2008.01140.x
PubMed:
18400000

6. Constitutive expression of RyhB regulates the heme biosynthesis pathway and increases the 5‐aminolevulinic acid accumulation in Escherichia coli

Author:
Li, Fangfang; Wang, Yang; Gong, Kai; Wang, Qian; Liang, Quanfeng; Qi, Qingsheng
Source:
FEMS microbiology letters 2014 v.350 no.2 pp. 209-215
ISSN:
0378-1097
Subject:
Escherichia coli; batch fermentation; biosynthesis; chelating agents; gene expression; genes; heme; iron; messenger RNA; reverse transcriptase polymerase chain reaction
Abstract:
... In the current study, the small RNA ryhB, which regulates the metabolism of iron in Escherichia coli, was constitutively expressed in engineered E. coli DALA. The resulting strain E. coli DALRA produced 16% more 5‐aminolevulinic acid (ALA) than the parent strain E. coli DALA in batch fermentation. Meanwhile, we found that addition of iron in the medium increased heme formation and reduced ALA yiel ...
DOI:
10.1111/1574-6968.12322

7. Evaluation of three reference genes of Escherichia coli for mRNA expression level normalization in view of salt and organic acid stress exposure in food

Author:
Peng, Silvio; Stephan, Roger; Hummerjohann, Jörg; Tasara, Taurai
Source:
FEMS microbiology letters 2014 v.355 no.1 pp. 78-82
ISSN:
0378-1097
Subject:
Escherichia coli; essential genes; gene expression; messenger RNA; models; proteins; quantitative polymerase chain reaction; reverse transcriptase polymerase chain reaction; reverse transcription; stress response
Abstract:
... Escherichia coli can adapt to various stress conditions encountered in food through induction of stress response genes encoding proteins that counteract the respective stresses. To understand the impact and the induction of these genes under food‐associated stresses, changes in the levels of their mRNA expression in response to such stresses can be analysed. Relative quantification of mRNA levels ...
DOI:
10.1111/1574-6968.12447