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- Zygmunt, Michel S., et al. Show all 3 Authors
- FEMS microbiology letters 1990 v.70 no.3 pp. 263-268
- Brucella melitensis; antigens; bacteria; goats; immune response; immunoblotting; isoelectric focusing; pH; polyacrylamide gel electrophoresis; proteins; silver
- ... Analysis of the immune response towards the facultative intracellular bacterium, Brucella melitensis, was studied by immunoblotting after either isoelectric focusing (IEF) or sodium dodecyl sulfateâpolyacrylamide gel electrophoresis (SDSâPAGE). A cytoplasmic extract (CPE) of Brucella melitensis was used as antigen to analyse the response in 17 sera from naturally infected goats. CPE analysed b ...