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- Author:
- Liu, Yichen; Song, Qiaozhen; Li, Danlei; Zou, Ruifeng; Zhang, Yichen; Hao, Shaoyan; Geng, Xuyun; Sun, Jinsheng
- Source:
- Fish & shellfish immunology 2019 v.91 pp. 376-387
- ISSN:
- 1050-4648
- Subject:
- Bacillus thuringiensis; Litopenaeus vannamei; RNA interference; Vibrio anguillarum; Vibrio parahaemolyticus; amino acids; bacterial infections; complement; complementary DNA; erythrocytes; gene expression; genes; gills; hemocytes; immune response; innate immunity; models; mortality; open reading frames; pathogens; protective effect; proteolysis; rabbits; shrimp; signal peptide; thioesters; transcriptional activation
- Abstract:
- ... As a core component of the complement system, complement component 3 (C3) plays a central role in the opsonization of pathogens, immune defense and immune regulation in the mammalian for its activation is required to trigger classical as well as alternative complement pathways. However, the molecular mechanism underlying C3 activation in invertebrates remains unknown. Several C3 genes have been ch ...
- DOI:
- 10.1016/j.fsi.2019.05.045
-
https://dx.doi.org/10.1016/j.fsi.2019.05.045
- Author:
- Yang, Wanying; Liu, Wenxiu; Wen, Chungen; Hu, Baoqing; Jian, Shaoqing; Gang, Yang
- Source:
- Fish & shellfish immunology 2019 v.91 pp. 180-187
- ISSN:
- 1050-4648
- Subject:
- Escherichia coli; amino acid sequences; amino acids; complementary DNA; enzyme activity; enzyme stability; freshwater mussels; gene expression; gene expression regulation; gills; hemocytes; hepatopancreas; immunology; manganese; messenger RNA; microcystins; muscles; open reading frames; pH; plasmids; quantitative polymerase chain reaction; recombinant proteins; sequence homology; shellfish; superoxide dismutase; temperature; urea
- Abstract:
- ... Manganese superoxide dismutase (MnSOD) is a sort of important metalloenzyme that can catalyze ROS in the organisms. In this study, MnSOD cDNA of C. plicata, designated as CpMnSOD (accession no. MK465057), was cloned from hemocytes. The full-length cDNA of MnSOD was 1096 bp with a 672 bp open reading frame encoding 223 amino acids. The deduced amino acid sequence contained a mitochondrial-targeting ...
- DOI:
- 10.1016/j.fsi.2019.04.307
-
https://dx.doi.org/10.1016/j.fsi.2019.04.307
- Author:
- Han, Zirong; Wang, Weilin; Lv, Xiaojing; Zong, Yanan; Liu, Shujing; Liu, Zhaoqun; Wang, Lingling; Song, Linsheng
- Source:
- Fish & shellfish immunology 2019 v.91 pp. 325-332
- ISSN:
- 1050-4648
- Subject:
- Crassostrea gigas; Crassostrea virginica; Octopodidae; amino acids; autophagy; cell viability; complementary DNA; double-stranded RNA; gene expression; gene expression regulation; gills; hemocytes; hepatopancreas; immune response; interferon regulatory factors; interferons; messenger RNA; open reading frames; oysters; polypeptides; shellfish
- Abstract:
- ... Autophagy, a highly conserved intracellular degradation system, is involved in numerous processes in vertebrate and invertebrate, such as cell survival, ageing, and immune responses. However, the detailed molecular mechanism of autophagy and its immune regulatory role in bivalves are still not well understood. In the present study, an autophagy-related protein ATG10 (designated as CgATG10) was ide ...
- DOI:
- 10.1016/j.fsi.2019.05.027
-
https://dx.doi.org/10.1016/j.fsi.2019.05.027
- Author:
- Cao, Yongsheng; Wang, Di; Li, Shaowu; Zhao, Jingzhuang; Xu, Liming; Liu, Hongbai; Lu, Tongyan; Mou, Zhenbo
- Source:
- Fish & shellfish immunology 2019 v.91 pp. 350-357
- ISSN:
- 1050-4648
- Subject:
- Aeromonas salmonicida subsp. salmonicida; Oncorhynchus mykiss; fish diseases; furunculosis; gene expression regulation; gene ontology; genes; high-throughput nucleotide sequencing; immune response; interleukin-1beta; interleukin-2; microRNA; non-coding RNA; pathogens; salmon; spleen; transcriptomics; trout
- Abstract:
- ... MicroRNAs (miRNAs) are a class of small non-coding RNAs that can regulate the immune responses during pathogen infection. Aeromonas salmonicida (A. salmonicida) subsp. salmonicida is the causative agent of furunculosis in salmon and trout. To identify the miRNAs and investigate the specific miRNAs in rainbow trout upon A. salmonicida subsp. salmonicida infection, we performed high throughput seque ...
- DOI:
- 10.1016/j.fsi.2019.05.048
-
https://dx.doi.org/10.1016/j.fsi.2019.05.048
- Author:
- Li, Huifeng; Xu, Chang; Zhou, Li; Dong, Yangfan; Su, Yujie; Wang, Xiaodan; Qin, Jian G.; Chen, Liqiao; Li, Erchao
- Source:
- Fish & shellfish immunology 2019 v.91 pp. 315-324
- ISSN:
- 1050-4648
- Subject:
- Flavobacterium; Geobacillus; Litopenaeus vannamei; Microbacterium; Mycobacterium; Toll-like receptors; Vibrio; amylases; antioxidant activity; beta-glucans; condition factor; diet; digestibility; enzyme activity; glutathione peroxidase; growth performance; health status; heat-shock protein 70; hepatopancreas; immunity; intestines; lectins; messenger RNA; microbial communities; myostatin; necrosis; neoplasms; pathogens; probiotics; proteinases; salinity; superoxide dismutase; tumor necrosis factor-alpha
- Abstract:
- ... An 8-week trial was conducted to evaluate the effect of dietary β-glucan supplement (0, 0.01%, 0.02%, or 0.04%) on growth and health of Pacific white shrimp Litopenaeus vannamei at low salinity of 3 practical salinity unit (psu). The L. vannamei fed 0.02% and 0.04% β-glucan gained more weight and showed higher activities of protease, amylase, superoxide dismutase, and glutathione peroxidase in the ...
- DOI:
- 10.1016/j.fsi.2019.05.052
-
https://dx.doi.org/10.1016/j.fsi.2019.05.052
- Author:
- Ozturk, Rafet C.; Altinok, Ilhan; Turgut, Secil; Capkin, Erol
- Source:
- Fish & shellfish immunology 2019 v.91 pp. 264-274
- ISSN:
- 1050-4648
- Subject:
- Lactococcus garvieae; Salmo trutta; Yersinia ruckeri; antioxidants; disease resistance; fish; gene expression; hybridization; hybrids; immunoglobulin light chains; major histocompatibility complex; purebreds; yersiniosis; Black Sea; Turkey (country)
- Abstract:
- ... Brown trout are polymorphic salmonid species, and it is of importance to investigate whether hybridization affects disease resistance. In this study, susceptibility of brown trout (Salmo trutta Abant, Anatolian, Black Sea, and Caspius) strains and their hybrids to Lactococcus garvieae and Yersinia ruckeri as well as their immune-related gene expression profiles were studied. Results indicated that ...
- DOI:
- 10.1016/j.fsi.2019.05.047
-
https://dx.doi.org/10.1016/j.fsi.2019.05.047
- Author:
- Chen, Xingxing; Han, Kun; Lin, Gang; Liu, Changxin; Wang, Shanghong; Shi, Xiao; Hu, Zhizhen; Wu, Chuxin; Xu, Xiaowen; Hu, Chengyu
- Source:
- Fish & shellfish immunology 2019 v.91 pp. 194-201
- ISSN:
- 1050-4648
- Subject:
- B-cell lymphoma; Ctenopharyngodon idella; apoptosis; autophagy; cell proliferation; cell viability; fish; gene expression; genes; growth factors; humans; immune response; interleukin-6; kidney cells; messenger RNA; metastasis; signal transduction; transactivators; tunicamycin
- Abstract:
- ... In mammals, STAT3 (Signal transducer and activator of transcription 3) plays an absolutely vital role in response to cytokines and growth factors. In mammals, IL-6/JAK/STAT3 pathway is closely linked to immune response and promotes cell proliferation, survival and metastasis. Some recent studies have already demonstrated that STAT3 regulates autophagy. As a downstream target gene of STAT3, Bcl-2 ( ...
- DOI:
- 10.1016/j.fsi.2019.05.034
-
https://dx.doi.org/10.1016/j.fsi.2019.05.034
- Author:
- Huang, Yupeng; Yuan, Xiaoqin; Mu, Pengfei; Li, Qiuhua; Ao, Jingqun; Chen, Xinhua
- Source:
- Fish & shellfish immunology 2019 v.91 pp. 216-222
- ISSN:
- 1050-4648
- Subject:
- B-lymphocytes; Larimichthys crocea; Western blotting; blood serum; fish; flow cytometry; fluorescent antibody technique; genetic markers; immunoglobulin M; kidneys; mice; monoclonal antibodies; phagocytosis; reverse transcriptase polymerase chain reaction; spleen
- Abstract:
- ... In the present study, a monoclonal antibody (mAb) against large yellow croaker IgM was produced by immunizing mice with purified large yellow croaker serum IgM. Western blotting showed that this mAb could specifically react with the heavy chain of large yellow croaker serum IgM. Indirect immunofluorescence assay (IFA) analysis suggested that the resulting mouse anti-IgM mAb could recognize membran ...
- DOI:
- 10.1016/j.fsi.2019.05.035
-
https://dx.doi.org/10.1016/j.fsi.2019.05.035
- Author:
- Naderi Farsani, Mehdi; Hoseinifar, Seyed Hossein; Rashidian, Ghasem; Ghafari Farsani, Hamed; Ashouri, Ghasem; Van Doan, Hien
- Source:
- Fish & shellfish immunology 2019 v.91 pp. 233-240
- ISSN:
- 1050-4648
- Subject:
- Coriandrum sativum; Oncorhynchus mykiss; Yersinia ruckeri; bacterial infections; complement; condition factor; coriander seed; disease resistance; fish; growth factors; growth performance; hematocrit; hemoglobin; innate immunity; lysozyme; physiological response; seed extracts; specific growth rate; survival rate
- Abstract:
- ... This investigation was aimed to determine the efficacy of coriander seed extract (Coriandrum sativum) on physiological responses, immunity and disease resistance of rainbow trout, Oncorhynchus mykiss for eight weeks. A total number of six hundred rainbow trout (62 ± 0.81 g) were divided into four feeding groups including 0 (control), 0.5%, 1% and 2% of coriander seed extract (CSE). In the present ...
- DOI:
- 10.1016/j.fsi.2019.05.031
-
https://dx.doi.org/10.1016/j.fsi.2019.05.031
- Author:
- Stenberg, Oda Kvalsvik; Holen, Elisabeth; Piemontese, Luisa; Liland, Nina S.; Lock, Erik-Jan; Espe, Marit; Belghit, Ikram
- Source:
- Fish & shellfish immunology 2019 v.91 pp. 223-232
- ISSN:
- 1050-4648
- Subject:
- Hermetia illucens; Salmo salar; antioxidants; aquaculture feeds; experimental diets; fish feeding; fish meal; fish oils; gene expression; gene expression regulation; genes; inflammation; ingredients; insects; interleukin-10; interleukin-1beta; interleukin-8; kidney cells; kidneys; larvae; leukocytes; lipopolysaccharides; salmon; seawater; stress response; superoxide dismutase; transcription (genetics); tumor necrosis factor-alpha; vegetable oil
- Abstract:
- ... With the fast growth of today's aquaculture industry, the demand for aquafeeds is expanding dramatically. Insects, which are part of the natural diet of salmonids, could represent a sustainable ingredient for aquaculture feed. The aim of the current study was to test how a partial or total replacement of dietary fishmeal with insect meal affect gene responses involved in inflammation, the eicosano ...
- DOI:
- 10.1016/j.fsi.2019.05.042
-
https://dx.doi.org/10.1016/j.fsi.2019.05.042
- Author:
- Jenabi Haghparast, R.; Sarvi Moghanlou, K.; Mohseni, M.; Imani, A.
- Source:
- Fish & shellfish immunology 2019 v.91 pp. 50-57
- ISSN:
- 1050-4648
- Subject:
- Salmo trutta; amylases; antioxidant enzymes; blood serum; carboxylic ester hydrolases; catalase; complement; digestive enzymes; enzyme activity; experimental diets; feed conversion; fish; glutathione transferase; immunoglobulin M; intestines; lecithins; leukocyte count; leukocytes; lysozyme; physiological response; proteinases; soybeans; specific growth rate; weight gain
- Abstract:
- ... A feeding experiment was conducted to evaluate the effects of dietary soybean lecithin (SBL) supplementation on performance, hemato-immunological parameters, lipid biochemistry, antioxidant status, digestive enzymes activity and intestinal histomorphometry of Caspian brown trout, Salmo trutta caspius in the pre-spawning stage. The basal diet was supplemented with 0% (control), 3%, 6%, 9% and 12% o ...
- DOI:
- 10.1016/j.fsi.2019.05.022
-
https://dx.doi.org/10.1016/j.fsi.2019.05.022
- Author:
- Singh, Soibam Khogen; Tiwari, V.K.; Chadha, N.K.; Munilkumar, Sukham; Prakash, Chandra; Pawar, Nilesh A.
- Source:
- Fish & shellfish immunology 2019 v.91 pp. 358-368
- ISSN:
- 1050-4648
- Subject:
- Bacillus circulans; Labeo rohita; acetylcholinesterase; antioxidant enzymes; biomarkers; blood chemistry; catalase; cortisol; dietary supplements; feed conversion; fish; fish feeding; fructooligosaccharides; glucose; glutathione transferase; growth performance; immune response; juveniles; lysozyme; pH; physiological state; probiotics; protein efficiency ratio; rearing; sodium-potassium-exchanging ATPase; specific growth rate; superoxide dismutase; weight gain
- Abstract:
- ... In this study, we investigated the effect of dietary Bacillus circulans PB7 (BCPB7) and fructoligosaccharide (FOS), used singly or in combination for evaluation of growth, immune and physiological status of Labeo rohita (rohu) juveniles reared under low pH and normal pH for 60 days. Experimental fishes were distributed in two sets such as one set continuously exposed to low pH (5.5) and other rear ...
- DOI:
- 10.1016/j.fsi.2019.05.023
-
https://dx.doi.org/10.1016/j.fsi.2019.05.023
- Author:
- Li, Mu-Yang; Guo, Wan-Qing; Guo, Gui-Liang; Zhu, Xin-Ming; Niu, Xiao-Tian; Shan, Xiao-Feng; Tian, Jia-Xin; Wang, Gui-Qin; Zhang, Dong-Ming
- Source:
- Fish & shellfish immunology 2019 v.91 pp. 122-129
- ISSN:
- 1050-4648
- Subject:
- Allium; Channa argus; antioxidants; bioaccumulation; catalase; complement; dietary supplements; fish; flavonoids; genes; glass; glucocorticoid receptors; glutathione peroxidase; immune response; immunoglobulin M; interleukin-1beta; interleukin-8; liver; lysozyme; malondialdehyde; oxidative stress; protective effect; selenium; spleen; subchronic exposure; tanks; toxicity; transcription factor NF-kappa B; tumor necrosis factor-alpha
- Abstract:
- ... Selenium (Se) is a micronutrient that becomes toxic when present at higher concentrations in fish tissues. Allium mongolicum Regel flavonoids (AMRF) have been documented to possess antioxidant, immunoenhancement and anti-inflammation properties. The aim of this study was to investigate the protective effects and potential mechanisms of dietary supplementation of AMRF and Se exposure on oxidative s ...
- DOI:
- 10.1016/j.fsi.2019.05.002
-
https://dx.doi.org/10.1016/j.fsi.2019.05.002
- Author:
- Jami, Mohammad Javad; Abedian Kenari, Abdolmohammad; Paknejad, Hamed; Mohseni, Mahmoud
- Source:
- Fish & shellfish immunology 2019 v.91 pp. 202-208
- ISSN:
- 1050-4648
- Subject:
- Lactobacillus plantarum; Salmo trutta; additives; ash content; beta-glucans; blood serum; complement; condition factor; cortisol; diet; feed intake; food conversion; gene expression; genes; glucose; growth performance; immunoglobulin M; immunostimulants; innate immunity; interleukin-1beta; interleukin-8; intestines; kidneys; lipids; lysozyme; messenger RNA; oligosaccharides; probiotics; transcription (genetics); trout; water content
- Abstract:
- ... This study investigated the effect of individual and combination of dietary pre- and probiotics (β-glucan, 3 mg/g; mannan oligosaccharide (MOS), 4 mg/g; and Lactobacillus plantarum; 108 CFU/mg diet) on growth performance, blood immune parameters, expression of immune related genes, and intestinal microbial of Caspian trout (Salmo trutta caspius). On the basis of feeding with immunostimulant diets, ...
- DOI:
- 10.1016/j.fsi.2019.05.024
-
https://dx.doi.org/10.1016/j.fsi.2019.05.024
- Author:
- Ye, Qiao; Feng, Yongyong; Wang, Zhenlu; Zhou, Aiguo; Xie, Shaolin; Zhang, Yue; Xiang, Qiong; Song, Enfeng; Zou, Jixing
- Source:
- Fish & shellfish immunology 2019 v.91 pp. 29-39
- ISSN:
- 1050-4648
- Subject:
- Aeromonas hydrophila; Gelsemium elegans; Megalobrama amblycephala; alanine transaminase; alkaloids; amino acids; antioxidant enzymes; aspartate transaminase; body weight; catalase; complement; crude protein; dietary supplements; disease resistance; feed conversion; fish; fish feeding; gene expression regulation; genes; growth performance; immune response; immunoglobulin M; interferon-alpha; interleukin-10; interleukin-1beta; interleukin-8; kidneys; lactate dehydrogenase; lipids; liver; messenger RNA; muscles; protein efficiency ratio; specific growth rate; spleen; superoxide dismutase; survival rate; transforming growth factor beta; tumor necrosis factor-alpha; weight gain
- Abstract:
- ... The present study aim to investigate the effects of dietary Gelsemium elegans alkaloids supplementation in Megalobrama amblycephala. A basal diet supplemented with 0, 5, 10, 20 and 40 mg/kg G. elegans alkaloids were fed to M. amblycephala for 12 weeks. The study indicated that dietary 20 mg/kg and 40 mg/kg G. elegans alkaloids supplementation could significantly improve final body weight (FBW), we ...
- DOI:
- 10.1016/j.fsi.2019.05.026
-
https://dx.doi.org/10.1016/j.fsi.2019.05.026
- Author:
- Qiao, Guo; Xu, Chen; Sun, Qirui; Xu, De-Hai; Zhang, Mingming; Chen, Peng; Li, Qiang
- Source:
- Fish & shellfish immunology 2019 v.91 pp. 251-263
- ISSN:
- 1050-4648
- Subject:
- Achromobacter; Bacillus (bacteria); Liza; aldehydes; antioxidant enzymes; blood serum; catalase; diet; disease control; enzyme activity; fish diseases; gene expression regulation; growth performance; high-throughput nucleotide sequencing; immune response; immunostimulants; interleukin-8; intestinal microorganisms; major histocompatibility complex; mullet; oral administration; poly-3-hydroxybutyrate; quantitative polymerase chain reaction; reverse transcriptase polymerase chain reaction; specific growth rate; superoxide dismutase; transcription (genetics); vaccines; weight gain; China
- Abstract:
- ... Soiny mullet (Liza haematocheila) is an important economic fish species in China, but stress and diseases have seriously restricted its culture. There are no effective methods including vaccines to prevent or control these diseases. Alternative methods should be employed, such as using novel immunostimulant poly-β-hydroxybutyrate (PHB). The present study aimed to evaluate effects of dietary PHB su ...
- DOI:
- 10.1016/j.fsi.2019.05.038
-
https://dx.doi.org/10.1016/j.fsi.2019.05.038
- Author:
- Yun, Saekil; Giri, Sib Sankar; Kim, Hyoun Joong; Kim, Sang Geun; Kim, Sang Wha; Kang, Jung Woo; Han, Se Jin; Kwon, Jun; Oh, Woo Taek; Chi, Cheng; Jun, Jin Woo; Chang Park, Se
- Source:
- Fish & shellfish immunology 2019 v.91 pp. 12-18
- ISSN:
- 1050-4648
- Subject:
- Aeromonas hydrophila; Cobitidae; Cyprinidae; affordability; agglutination; aquaculture; fish; formalin; free radicals; immunogenicity; lysozyme; microbubbles; phagocytosis; superoxide dismutase; survival rate; vaccination; vaccines; virulence
- Abstract:
- ... Immunization by bath immersion is likely the simplest method of fish vaccination. Although the route of immunogenicity has not been fully identified, immersion vaccination is clearly a useful labor-saving technique. In this study, microbubble (MB) treatment was assessed for its ability to improve the efficacy of bath immersion vaccination in the cyprinid loach. MBs are commonly defined as minute p ...
- DOI:
- 10.1016/j.fsi.2019.05.021
-
https://dx.doi.org/10.1016/j.fsi.2019.05.021
- Author:
- Mu, Liangliang; Yin, Xiaoxue; Wu, Hairong; Han, Kailiang; Wu, Liting; Ding, Mingmei; Bian, Xia; Li, Bingxi; Fu, Shengli; Liang, Fang; Guo, Zheng; Ye, Jianmin
- Source:
- Fish & shellfish immunology 2019 v.91 pp. 68-77
- ISSN:
- 1050-4648
- Subject:
- Aeromonas hydrophila; Oreochromis niloticus; Streptococcus agalactiae; amino acid sequences; bacterial infections; catalytic activity; coagulation; complement; epidermal growth factor; fish; fish diseases; gene expression regulation; hepatocytes; inflammation; innate immunity; intestines; kidneys; lectins; liver; macrophages; messenger RNA; monocytes; open reading frames; polypeptides; protein domains; protein synthesis; serine proteinases; spleen; tissues
- Abstract:
- ... Mannose-binding lectin-associated serine protease-1 (MASP-1), a multifunctional serine protease, plays an important role in innate immunity which is capable of activating the lectin pathway of the complement system and also triggering coagulation cascade system. In this study, a MASP-1 homolog (OnMASP-1) was identified from Nile tilapia (Oreochromis niloticus) and characterized at expression and i ...
- DOI:
- 10.1016/j.fsi.2019.05.014
-
https://dx.doi.org/10.1016/j.fsi.2019.05.014
- Author:
- Jozwick, Anna K.S.; LaPatra, Scott E.; Graf, Joerg; Welch, Timothy J.
- Source:
- Fish & shellfish immunology 2019 v.91 pp. 306-314
- ISSN:
- 1050-4648
- Subject:
- Yersinia ruckeri; adaptive immunity; bacterial growth; binding sites; biofilm; biosynthesis; chemotaxis; fish; flagellin; flagellum; gene expression regulation; loci; mortality; mutagenesis; mutants; operon; pathogens; promoter regions; reverse transcriptase polymerase chain reaction; structural proteins; transcription (genetics); virulence
- Abstract:
- ... The flagellum is a complex surface structure necessary for a number of activities including motility, chemotaxis, biofilm formation and host attachment. Flagellin, the primary structural protein making up the flagellum, is an abundant and potent activator of innate and adaptive immunity and therefore expression of flagellin during infection could be deleterious to the infection process due to flag ...
- DOI:
- 10.1016/j.fsi.2019.05.036
-
https://dx.doi.org/10.1016/j.fsi.2019.05.036
- Author:
- Zhang, Yong-Sheng; Li, Fei-Xiang; Yao, Cui-Luan
- Source:
- Fish & shellfish immunology 2019 v.91 pp. 275-283
- ISSN:
- 1050-4648
- Subject:
- Litopenaeus vannamei; Marsupenaeus japonicus; RNA interference; adenosine triphosphate; amino acid sequences; amino acids; brain; complementary DNA; crustin; gene expression; glucose; glucose 1-phosphate; glycogen; glycogenolysis; hemocytes; hepatopancreas; immunology; lactic acid; lectins; mortality; muscles; open reading frames; phosphorylase; phylogeny; polypeptides; pyruvic acid; shrimp; stomach
- Abstract:
- ... Glycogen phosphorylase (GP, EC 2.4.1.1) catalyze the rate-limiting step in glycogenolysis in animals, forming glucose-1-phosphate from the terminal alpha-1,4-glycosidic bond. Therefore, GP plays a crucial role in carbohydrate metabolism. In the present study, the full-length cDNA sequence of GP (LvGP) was cloned from shrimp, Litopenaeus vannamei. The obtained 3242-bp LvGP cDNA sequence included a ...
- DOI:
- 10.1016/j.fsi.2019.05.043
-
https://dx.doi.org/10.1016/j.fsi.2019.05.043