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You searched for: Journal Fish & shellfish immunology Remove constraint Journal: Fish & shellfish immunology Publication Year 2018 Remove constraint Publication Year: 2018 Source 2018 v.78 Remove constraint Source: 2018 v.78
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1. Adaptive response of pearl oyster Pinctada fucata martensii to low water temperature stress

Author:
Wang, Qingheng; Liu, Ya; Zheng, Zhe; Deng, Yuewen; Jiao, Yu; Du, Xiaodong
Source:
Fish & shellfish immunology 2018 v.78 pp. 310-315
ISSN:
1050-4648
Subject:
Pinctada fucata; amino acid metabolism; amino acids; biosynthesis; cold stress; cold zones; energy; gene expression regulation; genes; immunity; lysosomes; models; oxidative phosphorylation; oysters; quantitative polymerase chain reaction; reverse transcriptase polymerase chain reaction; sequence analysis; shellfish; transcriptome; transcriptomics; unsaturated fatty acids; water temperature
Abstract:
... The pearl oyster Pinctada fucata martensii is a warm-water shellfish that is sensitive to cold environments. To investigate its potential adaptation to low-temperature stress, the selected line (SL) and based population (BP) were sampled to undergo transcriptome sequence. Results of transcriptome analysis showed 572 significant differentially expressed genes. The typical HSP70 and HSP40 exhibited ...
DOI:
10.1016/j.fsi.2018.04.049

2. A fibrinogen-related protein, LvFREP2, from Litopenaeus vannamei facilitates the clearance of Vibrio harveyi

Author:
Tian, Yushun; Chen, Ting; Luo, Peng; Huang, Wen; Huo, Da; Yun, Long; Hu, Chaoqun; Cheng, Chuhang
Source:
Fish & shellfish immunology 2018 v.78 pp. 364-371
ISSN:
1050-4648
Subject:
Gram-negative bacteria; Litopenaeus vannamei; Pseudomonas aeruginosa; Pseudomonas fluorescens; Staphylococcus aureus; Vibrio alginolyticus; Vibrio cholerae; Vibrio harveyi; Vibrio parahaemolyticus; Vibrio vulnificus; complementary DNA; gene expression regulation; genes; gills; immune response; messenger RNA; open reading frames; protein domains; proteins; signal peptide
Abstract:
... Fibrinogen-related proteins (FREPs) play a crucial role in invertebrate immune response. In this study, we acquired a novel fibrinogen-related protein gene in Litopenaeus vannamei coding for one kind of fibrinogen-related protein, designated as LvFREP2. The complete cDNA sequence of LvFREP2 was 1903 bp long, containing an open reading frame of 1479 bp coding for LvFREP2. The LvFREP2 protein contai ...
DOI:
10.1016/j.fsi.2018.05.007

3. A novel toll-like receptor from Mytilus coruscus is induced in response to stress

Author:
Xu, Mengshan; Wu, Jiong; Ge, Delong; Wu, Changwen; Changfeng Chi,; Lv, Zhenming; Liao, Zhi; Liu, Huihui
Source:
Fish & shellfish immunology 2018 v.78 pp. 331-337
ISSN:
1050-4648
Subject:
3' untranslated regions; 5' untranslated regions; Aeromonas hydrophila; Mytilus; Toll-like receptors; Vibrio parahaemolyticus; amino acids; bacterial infections; complementary DNA; copper; gene expression; gene expression regulation; heavy metals; hemocytes; hepatopancreas; innate immunity; lead; molecular weight; open reading frames; phylogeny; quantitative polymerase chain reaction; reverse transcriptase polymerase chain reaction; shellfish; signal transduction; stress response; transcription factors; transmembrane proteins; virulent strains
Abstract:
... Toll-like receptor (TLR) is considered to be an evolutionarily conserved transmembrane protein which promotes the Toll signal pathway to active the expression of transcription factors in the innate immunity of the organism. In this study, a full length of TLR homologue of 2525bp in Mytilus coruscus (named as McTLR-a, GenBank accession no: KY940571) was characterized. Its ORF was 1815 bp with a 5'u ...
DOI:
10.1016/j.fsi.2018.04.058

4. Bax inhibitor-1 from orange spotted grouper, Epinephelus coioides involved in viral infection

Author:
Cai, Jia; Wei, Shina; Lu, Yishan; Wu, Zaohe; Qin, Qiwei; Jian, Jichang
Source:
Fish & shellfish immunology 2018 v.78 pp. 91-99
ISSN:
1050-4648
Subject:
Epinephelus coioides; amino acids; caspase-3; cell death; endoplasmic reticulum; gene overexpression; genes; grouper; immunology; kidneys; pro-apoptotic proteins; spleen; transcription (genetics)
Abstract:
... Bax inhibitor-1 (BI-1) is a conserved anti-apoptotic protein that suppresses endoplasmic reticulum (ER) stress-induced cell death. However, the function of fish BI-1 is not quite clear. In the present study, a bi-1 homolog (Ecbi-1) from orange spotted grouper, Epinephelus coioides was identified and its roles in viral infection were investigated. EcBI-1 encoded 237 amino acids protein, contained s ...
DOI:
10.1016/j.fsi.2018.04.020

5. Changes in natural haemolytic complement activity induced by stress in gilthead seabream (Sparus aurata L.)

Author:
Guardiola, Francisco A.; Saraiva-Fraga, Miguel; Cuesta, Alberto; Esteban, M. Angeles
Source:
Fish & shellfish immunology 2018 v.78 pp. 317-321
ISSN:
1050-4648
Subject:
Sparus aurata; aquaculture; blood serum; clove oil; complement; farmed fish; mucus; risk
Abstract:
... In aquaculture, animals can be continually exposed to environmental stress factors that put their health and even survival at risk. Two experiments were performed to evaluate the impact of different stress conditions (acute crowding and anaesthetic) on the natural haemolytic complement activity in serum and skin mucus of gilthead seabream (Sparus aurata L.). In the first experiment, fish were subj ...
DOI:
10.1016/j.fsi.2018.04.056

6. Characterisation and functional comparison of single-CRD and multidomain containing galectins CgGal-2 and CgGal-3 from oyster Crassostrea gigas

Author:
Huang, Mengmeng; Zhou, Tao; Wu, Yuehong; Fei, Hui; Wang, Gaoyang; Li, Zhi; Lei, Yutong; Liu, Qian; Sun, Cong; Lv, Zhengbing; Xu, Xue-Wei
Source:
Fish & shellfish immunology 2018 v.78 pp. 238-247
ISSN:
1050-4648
Subject:
Crassostrea gigas; Escherichia coli; Gram-negative bacteria; Gram-positive bacteria; Komagataella pastoris; Micrococcus luteus; Saccharomyces cerevisiae; Vibrio vulnificus; amino acid sequences; amino acids; bacterial infections; binding capacity; carbohydrate binding; coagulation; consensus sequence; erythrocytes; fungi; galactose; galectins; glucans; immune response; innate immunity; lipopolysaccharides; open reading frames; oysters; pathogen-associated molecular patterns; peptidoglycans; polypeptides; shellfish
Abstract:
... Galectins are β-galactoside binding lectins that play crucial roles in innate immunity in vertebrates and invertebrates through their conserved carbohydrate-recognition domains (CRDs). In the present study, single- and four-CRD-containing galectins were identified in oyster Crassostrea gigas (designated CgGal-2 and CgGal-3). The open reading frames (ORFs) of CgGal-2 and CgGal-3 encode polypeptides ...
DOI:
10.1016/j.fsi.2018.04.029

7. Cloning, identification and function analysis of a Chibby homolog from Litopenaeus vannamei

Author:
Ruan, Lingwei; Sun, Jiazhen; Zhou, Congzhao; Shi, Hong; Lei, Kaiyu
Source:
Fish & shellfish immunology 2018 v.78 pp. 114-120
ISSN:
1050-4648
Subject:
Litopenaeus vannamei; beta catenin; cell differentiation; fluorescent antibody technique; gene expression regulation; gills; heart; hemocytes; hepatopancreas; immune system; intestines; muscles; quantitative polymerase chain reaction; sequence analysis; signal transduction; stem cells; virus replication; viruses
Abstract:
... Chibby, a vital inhibitor molecule of Wnt/β-catenin signaling pathway, participates in development and stem cell differentiation through the regulation of β-catenin. Our previous studies have demonstrated that Litopenaeus vannamei β-catenin (Lv-β-catenin) was involved in WSSV infection and could inhibit virus replication by modulating the host immune system. In the study, a Chibby homolog from L. ...
DOI:
10.1016/j.fsi.2018.04.039

8. Development of monoclonal antibodies against IgM of sea bass (Lateolabrax japonicus) and analysis of phagocytosis by mIgM+ lymphocytes

Author:
Yang, Shun; Tang, Xiaoqian; Sheng, Xiuzhen; Xing, Jing; Zhan, Wenbin
Source:
Fish & shellfish immunology 2018 v.78 pp. 372-382
ISSN:
1050-4648
Subject:
B-lymphocytes; Lactococcus lactis; Lateolabrax japonicus; Western blotting; bass; binding capacity; blood serum; enzyme-linked immunosorbent assay; flow cytometry; fluorescence; fluorescent antibody technique; hybridomas; immunoglobulin M; marine fish; microparticles; models; monoclonal antibodies; phagocytosis; spleen
Abstract:
... B cells in some fish were recently found to have potent phagocytic activities. Sea bass (Lateolabrax japonicus) as an important economical marine fish species, it could be used as an appropriate model to study the functions of B cells in phagocytosis. In the paper, three positive hybridomas designated as 1E11, 2H4 and 3F3 secreting monoclonal antibodies (MAbs) against sea bass immunoglobulin M (Ig ...
DOI:
10.1016/j.fsi.2018.04.042

9. Dietary effects of succinic acid on the growth, digestive enzymes, immune response and resistance to ammonia stress of Litopenaeus vannamei

Author:
Duan, Yafei; Wang, Yun; Zhang, Jiasong; Sun, Yongxu; Wang, Jun
Source:
Fish & shellfish immunology 2018 v.78 pp. 10-17
ISSN:
1050-4648
Subject:
Litopenaeus vannamei; ammonia; amylases; anti-infective agents; aquaculture; carboxylic ester hydrolases; dietary supplements; enzyme activity; feed additives; gene expression; genes; growth promotion; hepatopancreas; immune response; pepsin; shrimp; succinic acid; superoxide dismutase; survival rate; trypsin
Abstract:
... Organic acids acts as an growth promoter and antimicrobial agent in aquaculture. The present study investigated the effects of a natural organic acid - succinic acid (SA) on the growth, digestive enzymes, immune response and resistance to ammonia stress of Litopenaeus vannamei. The shrimps were firstly fed with diets containing different levels of SA: 0% (Control), 0.25% (SA1), 0.50% (SA2), and 1. ...
DOI:
10.1016/j.fsi.2018.04.008

10. Distribution of mannose receptor in blunt snout bream (Megalobrama amblycephala) during the embryonic development and its immune response to the challenge of Aeromonas hydrophila

Author:
Wu, Changsong; Zhao, Xiaoheng; Babu V, Sarath; Yuan, Gailing; Wang, Weimin; Su, Jianguo; Liu, Xiaoling; Lin, Li
Source:
Fish & shellfish immunology 2018 v.78 pp. 52-59
ISSN:
1050-4648
Subject:
Aeromonas hydrophila; Megalobrama amblycephala; anti-inflammatory activity; bacteria; brain; complementary DNA; eggs; embryogenesis; fish; gene expression; genes; gills; heart; immune response; immunohistochemistry; intestines; kidneys; liver; macrophages; mannose; messenger RNA; muscles; pathogens; phagocytosis; polyclonal antibodies; protein content; quantitative polymerase chain reaction; rabbits; reverse transcriptase polymerase chain reaction; spleen; surveys; transmembrane proteins; tumor necrosis factor-alpha; yolk sac
Abstract:
... The mannose receptor (MR) is a type I transmembrane protein. Its ectodomain has eight C-type lectin-like domains, which are able to recognize and mediate the phagocytosis of a wide range of pathogens. Comprehensive studies have revealed that mammalian MR is widely distributed in the mononuclear phagocyte system (MPS, previously known as the reticuloendothelial system) and play a key role both in t ...
DOI:
10.1016/j.fsi.2018.03.049

11. Effect of antiaggregants on the in vitro viability, cell count and stability of abalone (Haliotis iris) haemocytes

Author:
Grandiosa, Roffi; Bouwman, Mai-Louise; Young, Tim; Mérien, Fabrice; Alfaro, Andrea C.
Source:
Fish & shellfish immunology 2018 v.78 pp. 131-139
ISSN:
1050-4648
Subject:
Haliotis iris; abalone; ambient temperature; blood sampling; cell viability; cost effectiveness; flow cytometry; hemocytes; heparin; immune system; lithium; microscopy; mixing; shellfish
Abstract:
... The ability to successfully prepare and preserve haemocyte cells for microscopy and flow cytometry is critical for the investigation of animal immune systems. In this study, we observed the total cell count, in vitro viability and stability of New Zealand black-footed abalone (Haliotis iris) haemocytes with different antiaggregants and handling protocols. Haemocyte stability was evaluated by direc ...
DOI:
10.1016/j.fsi.2018.04.038

12. Effect of high dietary starch levels on growth, hepatic glucose metabolism, oxidative status and immune response of juvenile largemouth bass, Micropterus salmoides

Author:
Lin, Shi-Mei; Shi, Chao-Ming; Mu, Ming-Ming; Chen, Yong-Jun; Luo, Li
Source:
Fish & shellfish immunology 2018 v.78 pp. 121-126
ISSN:
1050-4648
Subject:
Micropterus salmoides; alanine; alanine transaminase; alkaline phosphatase; antioxidant activity; aspartate transaminase; body weight; catalase; dietary carbohydrate; energy; feed conversion; fish; fructose-bisphosphatase; glucokinase; glucose; glycogen; growth performance; health status; immune response; inducible nitric oxide synthase; innate immunity; insulin; juveniles; liver; malondialdehyde; metabolism; muscles; nitric oxide; oxidative stress; phosphofructokinases; protein efficiency ratio; pyruvate carboxylase; pyruvate kinase; specific growth rate; starch; superoxide dismutase; triacylglycerols; weight gain; wheat starch
Abstract:
... An experimental trial was conducted to investigate the effects of high dietary starch levels on growth, hepatic glucose metabolism enzyme, antioxidant capacity and immune responses of largemouth bass, Micropterus salmoides. Fish (initial body weight: 16.9 ± 0.24 g) were fed three isonitrogenous and isoenergetic semi-purified diets containing 5%, 10% and 20% wheat starch, respectively. The results ...
DOI:
10.1016/j.fsi.2018.04.046

13. Effect of immunization of rohu Labeo rohita with inactivated germinated zoospores in providing protection against Aphanomyces invadans

Author:
Pradhan, Pravata K.; Sood, Neeraj; Yadav, Manoj K.; Arya, Pooja; Chaudhary, Dharmendra K.; Kumar, Uday; Kumar, Chandra Bhushan; Swaminathan, T.R.; Rathore, Gaurav
Source:
Fish & shellfish immunology 2018 v.78 pp. 195-201
ISSN:
1050-4648
Subject:
Aphanomyces invadans; Labeo rohita; Western blotting; adjuvants; antibodies; antigens; carp; disease control; enzyme activity; fish diseases; freshwater fish; germination; granuloma; histopathology; hyphae; immune response; mortality; muscle tissues; peptides; secondary immunization; zoospores; India
Abstract:
... Infection with Aphanomyces invadans is one of the most destructive diseases of freshwater fishes. Indian major carps, the dominant cultured species in the Indian sub-continent are highly susceptible to this disease. Till date, there is no effective treatment for control of this disease and immunization can be one of the strategies to reduce disease-related losses. In the present study, inactivated ...
DOI:
10.1016/j.fsi.2018.04.041

14. Effects of dietary arginine on antioxidant status and immunity involved in AMPK-NO signaling pathway in juvenile blunt snout bream

Author:
Liang, Hualiang; Ji, Ke; Ge, Xianping; Ren, Mingchun; Liu, Bo; Xi, Bingwen; Pan, Liangkun
Source:
Fish & shellfish immunology 2018 v.78 pp. 69-78
ISSN:
1050-4648
Subject:
Aeromonas hydrophila; Megalobrama amblycephala; adenosine monophosphate; antioxidant activity; antioxidants; arginine; complement; copper; diet; fish; fish feeding; gene expression; genes; glutathione peroxidase; immune response; immunoglobulin M; inducible nitric oxide synthase; inflammation; interleukin-1beta; interleukin-8; juveniles; malondialdehyde; messenger RNA; mortality; necrosis; neoplasms; nitric oxide; oxidative stress; protein kinases; signal transduction; sorbents; superoxide dismutase; tissues; tumor necrosis factor-alpha
Abstract:
... The present study assessed the effects of dietary arginine on antioxidant status and immunity involved in AMPK-NO signaling pathway in juvenile blunt snout bream. Fish were fed six practical diets with graded arginine levels ranging from 0.87% to 2.70% for 8 weeks. The results showed that compared with the control group (0.87% dietary arginine level), significantly higher mRNA levels of adenosine ...
DOI:
10.1016/j.fsi.2018.04.028

15. Effects of dietary toxic cyanobacteria and ammonia exposure on immune function of blunt snout bream (Megalabrama amblycephala)

Author:
Xia, Hu; Song, Ting; Wang, Li; Jiang, Liangsen; Zhou, Qiting; Wang, Weimin; Liu, Liangguo; Yang, Pinhong; Zhang, Xuezhen
Source:
Fish & shellfish immunology 2018 v.78 pp. 383-391
ISSN:
1050-4648
Subject:
Cyanobacteria; Megalobrama amblycephala; ammonia; ammonium nitrogen; brain; diet; eutrophication; fish; freeze drying; genes; gills; heart; immune response; immunotoxicity; intestines; kidneys; liver; lymphocytes; macrophages; microcystins; mitochondria; muscles; phagocytosis; respiratory burst; spleen; synergism; transcription (genetics)
Abstract:
... Cyanobacterial blooms caused by water eutrophication have become a worldwide problem. During the degradation of toxic cyanobacterial blooms, elevated ammonia and microcystins concentrations co-occur and exert toxicity on fish. Up to now, the combined effect of microcystins and ammonia on fish immunotoxicity has not been reported. The present study investigated immune responses of blunt snout bream ...
DOI:
10.1016/j.fsi.2018.04.023

16. Effects of dietary trehalose on the growth performance and nonspecific immunity of white shrimps (Litopenaeus vannamei)

Author:
Zhu, Ming; Long, Xiaohua; Wu, Shengjun
Source:
Fish & shellfish immunology 2018 v.78 pp. 127-130
ISSN:
1050-4648
Subject:
Litopenaeus vannamei; Vibrio alginolyticus; body weight; breeding; disease resistance; enzyme activity; experimental diets; glutathione peroxidase; growth performance; hemocytes; immunity; immunostimulants; monophenol monooxygenase; oral administration; shrimp; superoxide dismutase; survival rate; trehalose
Abstract:
... This study aimed to investigate the effects of trehalose on the growth performance and nonspecific immunity of white shrimps (Litopenaeus vannamei). The shrimps were randomly divided into four groups, i.e. control group fed with normal commercial diet and three treatment groups fed with trehalose-containing diets (0.5, 1 and 1.5 g kg−1) and experimental diets during a 12-week breeding experiment. ...
DOI:
10.1016/j.fsi.2018.04.035

17. Efficacy of recombinant protein vaccines for protection against Nocardia seriolae infection in the largemouth bass Micropterus salmoides

Author:
Ho, Ping-Yueh; Chen, Yao-Chung; Maekawa, Shun; Hu, Hsiang-Hui; Tsai, An-Wei; Chang, Yung-Fu; Wang, Pei-Chi; Chen, Shih-Chu
Source:
Fish & shellfish immunology 2018 v.78 pp. 35-41
ISSN:
1050-4648
Subject:
ABC transporters; Escherichia coli; Micropterus salmoides; Nocardia seriolae; antigens; blood serum; disease control; esterases; fish; fish diseases; genes; nocardiosis; protein synthesis; recombinant proteins; subunit vaccines; surveys; vaccination; virulent strains
Abstract:
... A reverse vaccinology-based survey of potent antigens associated with fish nocardiosis was conducted using the largemouth bass, Micropterus salmoides, with an aim to develop subunit vaccines. The antigens selected from the virulent strain Nocardia seriolae 961113 include the gene products of NGL2579 (GAPDH), NGL5701 (MMP), NGL4377 (OCTase), NGL4486 (ABC transporter), NGL3372 (LLE), NGL3388 (GHf10) ...
DOI:
10.1016/j.fsi.2018.04.024

18. Epigallocatechin-3-gallate protects Kuruma shrimp Marsupeneaus japonicus from white spot syndrome virus and Vibrio alginolyticus

Author:
Wang, Zhi; Sun, Baozhen; Zhu, Fei
Source:
Fish & shellfish immunology 2018 v.78 pp. 1-9
ISSN:
1050-4648
Subject:
Gram-negative bacteria; Marsupenaeus japonicus; Vibrio alginolyticus; White spot syndrome virus; antineoplastic activity; apoptosis; catechin; enzyme activity; epigallocatechin gallate; genes; green tea; hemocytes; innate immunity; mitogen-activated protein kinase; models; monophenol monooxygenase; mortality; myosin; pathogens; reverse transcriptase polymerase chain reaction; shrimp; superoxide dismutase; therapeutics; tumor necrosis factor-alpha
Abstract:
... Epigallocatechin-3-gallate (EGCG) is the most abundant catechin in green tea and exhibits potential antibacterial and anticancer activities. In this study, EGCG was used in pathogen-challenge experiments in shrimp to discover its effect on the innate immune system of an invertebrate. Kuruma shrimp Marsupeneaus japonicus was used as an experimental model and challenged with white spot syndrome viru ...
DOI:
10.1016/j.fsi.2018.04.021

19. Expression profiling and microbial ligand binding analysis of high-mobility group box-1 (HMGB1) in turbot (Scophthalmus maximus L.)

Author:
Cai, Xin; Gao, Chengbin; Su, Baofeng; Tan, Fenghua; Yang, Ning; Wang, Guodong
Source:
Fish & shellfish immunology 2018 v.78 pp. 100-108
ISSN:
1050-4648
Subject:
DNA-binding proteins; Larimichthys crocea; Scophthalmus maximus; amino acids; binding capacity; brain; complementary DNA; cytokines; genes; gills; humans; immune response; inflammation; intestines; kidneys; ligands; molecular weight; mucosa; mucosal immunity; nucleosomes; open reading frames; phylogeny; polypeptides; transcription (genetics); turbot
Abstract:
... High-mobility group box 1 (HMGB1), a highly conserved DNA-binding protein, was involved in nucleosome formation and transcriptional regulation, and could also act as an extracellular cytokine to trigger inflammation and immune responses. In this study, we identified a HMGB1 gene in turbot (Scophthalmus maximus L.). The full-length SaHMGB1 cDNA includes an open reading frame of 615 bp which encoded ...
DOI:
10.1016/j.fsi.2018.04.025

20. Fishmeal replacement by rice protein concentrate with xylooligosaccharides supplement benefits the growth performance, antioxidant capability and immune responses against Aeromonas hydrophila in blunt snout bream (Megalobrama amblycephala)

Author:
Abasubong, Kenneth Prudence; Liu, Wen-Bin; Zhang, Ding-Dong; Yuan, Xiang-Yang; Xia, Si-Lei; Xu, Chao; Li, Xiang-Fei
Source:
Fish & shellfish immunology 2018 v.78 pp. 177-186
ISSN:
1050-4648
Subject:
Aeromonas hydrophila; Megalobrama amblycephala; acid phosphatase; alkaline phosphatase; antioxidant activity; bacterial infections; binding capacity; blood; catalase; complement; dietary supplements; enzyme activity; fish; fish feeding; fish meal; glutathione peroxidase; growth performance; immune response; immunoglobulin M; innate immunity; iron; liver; lysozyme; malondialdehyde; myeloperoxidase; nutritional intervention; protein concentrates; protein efficiency ratio; rice protein; superoxide dismutase; weight gain; xylooligosaccharides
Abstract:
... This study aimed to investigate the effects of fishmeal (FM) replacement by rice protein concentrate (RPC) with a xylooligosaccharides supplement on the growth performance, antioxidant capability and immune response against Aeromonas hydrophila in blunt snout bream (Megalobrama amblycephala). Fish (46.85 ± 0.34 g) were randomly assigned to one of 6 diets, namely the control diet (containing FM), t ...
DOI:
10.1016/j.fsi.2018.04.044