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- Author:
- Jia, Yudong; Jing, Qiqi; Gao, Yunhong; Huang, Bin
- Source:
- Fish physiology and biochemistry 2019 v.45 no.3 pp. 955-964
- ISSN:
- 0920-1742
- Subject:
- turbot, etc ; Scophthalmus maximus; abnormal development; animal ovaries; animal reproduction; binding proteins; breeding stock; gene expression; gonadosomatic index; hepatosomatic index; hormone receptors; insulin-like growth factor I; insulin-like growth factor I receptor; liver; messenger RNA; migratory behavior; ovarian development; somatotropin; vitellogenesis; vitellogenin; Show all 20 Subjects
- Abstract:
- ... Accumulating evidence suggests that the growth hormone (GH)/insulin-like growth factor (IGF) system participates in fish reproduction. To understand the physiological functions of the GH/IGF system, the mRNA expression profiles of all known members of the GH/IGF system, including hepatic and ovarian gh, GH receptor (ghr), IGFs (igf-i, igf-ii), IGF-I receptor (igf-ir) and IGF binding protein (igfbp ...
- DOI:
- 10.1007/s10695-018-0604-z
-
http://dx.doi.org/10.1007/s10695-018-0604-z
- Author:
- Han, Dongdong; Miao, Huijun; Nie, Qin; Miao, Shuyan; Zhang, Qin; Zhang, Wenbing; Mai, Kangsen
- Source:
- Fish physiology and biochemistry 2016 v.42 no.6 pp. 1665-1679
- ISSN:
- 0920-1742
- Subject:
- turbot, etc ; Scophthalmus maximus; amino acid sequences; amino acids; brain; complementary DNA; diet; eyes; gene expression; grouper; humans; kidneys; leptin; liver; messenger RNA; nucleotide sequences; tissue distribution; Show all 17 Subjects
- Abstract:
- ... In the present study, the full-length cDNA sequences of leptin (LEP) and its receptor (LEPR) from turbot Scophthalmus maximus were cloned. The cDNA of tLEP was 1126 bp in length encoding 157 amino acids. The amino acid sequence shared low identity with human LEP (18.8 %), but the three-dimensional structures of these two LEPs were strongly conserved. The deduced 1173-amino acid sequence of tLEPR w ...
- DOI:
- 10.1007/s10695-016-0248-9
-
http://dx.doi.org/10.1007/s10695-016-0248-9
- Author:
- Chen, Yadong; Zhou, Shuhong; Jiang, Zhiqiang; Wang, Xiuli; Liu, Yang
- Source:
- Fish physiology and biochemistry 2016 v.42 no.2 pp. 659-671
- ISSN:
- 0920-1742
- Subject:
- turbot, etc ; CXCR3 receptor; CXCR5 receptor; Oncorhynchus mykiss; Scophthalmus maximus; amino acids; blood; chemokines; complementary DNA; gene expression; genes; immune response; kidneys; lipopolysaccharides; liver; molecular cloning; phylogeny; quantitative polymerase chain reaction; spleen; Show all 19 Subjects
- Abstract:
- ... Chemokine (C-X-C motif) receptor 3, a member of the G protein-coupled receptors superfamily, regulates the responses of many immune responses. In this experiment, we cloned and characterized the cDNA of CXCR3 in Scophthalmus maximus (turbot). A 5′-UTR of 216-bp, a 259-bp 3′-UTR with a poly (A) tail and a 1089-bp CDS encoding 362 amino acids form the cDNA of CXCR3, which is 1564-bp long. Phylogenet ...
- DOI:
- 10.1007/s10695-015-0167-1
-
http://dx.doi.org/10.1007/s10695-015-0167-1
- Author:
- Nie, Qin; Miao, Huijun; Miao, Shuyan; Zhou, Huihui; Zhang, Yanjiao; Zhang, Wenbing; Mai, Kangsen
- Source:
- Fish physiology and biochemistry 2015 v.41 no.3 pp. 819-832
- ISSN:
- 0920-1742
- Subject:
- turbot, etc ; Scophthalmus maximus; amino acids; brain; complementary DNA; dextrins; dietary carbohydrate; gene expression; genes; glucokinase; glucose; glycolysis; heart; intestines; kidneys; liver; messenger RNA; muscles; open reading frames; rapid amplification of cDNA ends; Show all 20 Subjects
- Abstract:
- ... Glucokinase (GK) and fructose-1,6-bisphosphatase (FBPase) play crucial role in glucose metabolism. In the present study, the cDNA encoding GK and FBPase was cloned from the liver of turbot Scophthalmus maximus by rapid amplification of cDNA end technique. Effects of dietary glucose and dextrin on the activities and gene expressions of these two enzymes were also studied. Results showed that the fu ...
- DOI:
- 10.1007/s10695-015-0049-6
-
http://dx.doi.org/10.1007/s10695-015-0049-6
- Author:
- Sun, Airong; Li, Jian; Huang, Jingzhou; Chang, Zhiqiang; Li, Jitao; Wang, Qi
- Source:
- Fish physiology and biochemistry 2013 v.39 no.5 pp. 1239-1251
- ISSN:
- 0920-1742
- Subject:
- turbot, etc ; sequence alignment; isoelectric point; Scophthalmus maximus; genes; oral administration; open reading frames; rapid amplification of cDNA ends; amino acids; spleen; signal peptide; stomach; molecular weight; messenger RNA; complementary DNA; cytochrome P-450; molecular cloning; kidneys; pharmacokinetics; liver; gall bladder; reverse transcriptase polymerase chain reaction; Show all 22 Subjects
- Abstract:
- ... In this study, the cytochrome P450 3A (CYP3A) gene was cloned from the turbot Scophthalmus maximus for the first time using reverse transcription-polymerase chain reaction (RT-PCR) and rapid amplification of cDNA ends approaches. The amino acid sequences were analyzed with corresponding software programs. The cDNA of CYP3A was 1,969 bp in length, which contained a 5′-untranslated region (UTR) of 3 ...
- DOI:
- 10.1007/s10695-013-9779-5
- PubMed:
- 23525829
-
http://dx.doi.org/10.1007/s10695-013-9779-5
- Author:
- Chen, S. L.; Liu, Y.; Dong, X. L.; Meng, L.
- Source:
- Fish physiology and biochemistry 2010 v.36 no.2 pp. 147-155
- ISSN:
- 0920-1742
- Subject:
- turbot, etc ; phylogeny; Scophthalmus maximus; open reading frames; bacteria; brain; rapid amplification of cDNA ends; leukocytes; spleen; embryogenesis; introns; tissues; chemokine CCL19; complementary DNA; cDNA libraries; immune response; kidneys; exons; liver; chemotaxis; Show all 20 Subjects
- Abstract:
- ... The chemokines are a superfamily of chemotactic cytokines playing an important role in leukocyte chemotaxis. Here, a turbot head kidney cDNA library was constructed in which KC70 was identified as a CC chemokine. Unknown 5′ and 3′ parts of the cDNA were amplified by 5′ and 3′ rapid amplification of cDNA ends (RACE). The complete cDNA of KC70 contains a 59-bp 5′ UTR, a 336-bp ORF, and a 152-bp 3′ U ...
- DOI:
- 10.1007/s10695-008-9218-1
- PubMed:
- 20467856
-
http://dx.doi.org/10.1007/s10695-008-9218-1