PubAg

Main content area

You searched for: Subject "turbot" Remove constraint Subject: "turbot" Journal Fish physiology and biochemistry Remove constraint Journal: Fish physiology and biochemistry
Start Over

Search

26  Search Results

« Previous | 1 - 20 of 26 |
Number of results to display per page

Search Results

1. Effect of triploidy on digestive enzyme activity of early stages of turbot (Scophthalmus maximus)

Author:
Domingues, P.; Hernández-Urcera, J.; Cal, R.; Olivares, A.; Chimal, M.; Sánchez, A.; Rosas, C.; Gallardo, P.
Source:
Fish physiology and biochemistry 2019 v.45 no.2 pp. 573-582
ISSN:
0920-1742
Subject:
turbot,   etc   ; Scophthalmus maximus; alpha-amylase; carboxylic ester hydrolases; diploidy; enzyme activity; hatching; pH; pellets; pepsin; proteolysis; temperature; triploidy; trypsin;     Show all 14 Subjects
Abstract:
... In the present work, growth and digestive enzyme activities of total acid and alkaline proteases, pepsin, trypsin, lipase, and α-amylase, as well as partial characterization of enzyme activity, were studied in diploid and triploid turbot. Growth was similar between both groups. Acid protease activity increased consistently during the experiment, for both diploid (2n) and triploid (3n) fish. The al ...
DOI:
10.1007/s10695-019-00610-z

2. The effect of a multi-strain probiotic on growth performance, non-specific immune response, and intestinal health of juvenile turbot, Scophthalmus maximus L.

Author:
Li, Zequn; Bao, Ning; Ren, Tongjun; Han, Yuzhe; Jiang, Zhiqiang; Bai, Zhuoan; Hu, Yanan; Ding, Jingyun
Source:
Fish physiology and biochemistry 2019 v.45 no.4 pp. 1393-1407
ISSN:
0920-1742
Subject:
turbot,   etc   ; Lactobacillus; Scophthalmus maximus; body composition; diet; enzyme activity; fish culture; growth performance; immune response; intestinal microorganisms; intestines; juveniles; lysozyme; probiotics; villi;     Show all 15 Subjects
Abstract:
... Probiotic is well known because of its health benefit on the host, including improve growth, treat disease, and enhance immunity. Currently, probiotic has been widely used in aquaculture. However, there is little information about the effect of probiotic on turbot. Therefore, an effort was made to explore the effect of a multi-strain probiotic on growth performance, non-specific immune response, a ...
DOI:
10.1007/s10695-019-00635-4

3. Network of microRNA-transcriptional factor-mRNA in cold response of turbot Scophthalmus maximus

Author:
Nie, Miaomiao; Tan, Xungang; Lu, Yunliang; Wu, Zhihao; Li, Jun; Xu, Dongdong; Zhang, Peijun; You, Feng
Source:
Fish physiology and biochemistry 2019 v.45 no.2 pp. 583-597
ISSN:
0920-1742
Subject:
turbot,   etc   ; Scophthalmus maximus; cell cycle; cell membranes; circadian rhythm; cold; cold stress; cold tolerance; diploidy; gene expression regulation; messenger RNA; microRNA; sequence analysis; signal transduction; transcription (genetics);     Show all 15 Subjects
Abstract:
... The aim of this study is to understand fish cold-tolerant mechanism. We analyzed the transcriptional reactions to the cold condition in turbot Scophthalmus maximus by using RNA-seq and microRNA (miRNA)-seq. Meio-gynogenetic diploid turbots were treated at 0 °C to distinguish the cold-tolerant (CT) and cold-sensitive (CS) groups. The results showed that there were quite different responses at both ...
DOI:
10.1007/s10695-019-00611-y

4. Establishment and characterization of a fibroblast-like cell line from the muscle of turbot (Scophthalmus maximus L.)

Author:
Gao, Ya; Zhou, Huihui; Gao, Zongyu; Jiang, Haowen; Wang, Xuan; Mai, Kangsen; He, Gen
Source:
Fish physiology and biochemistry 2019 v.45 no.3 pp. 1129-1139
ISSN:
0920-1742
Subject:
turbot,   etc   ; Scophthalmus maximus; amino acids; cell lines; cell viability; chromosome number; cytogenetic analysis; diploidy; fetal bovine serum; fibroblast growth factor 2; fibroblasts; juveniles; mortality; muscles; proteolysis; rapamycin; signal transduction; temperature; transcription factors; transfection; trypsin;     Show all 21 Subjects
Abstract:
... A continuous fibroblast-like cell line, TMF (turbot muscle fibroblasts), was established from juvenile turbot Scophthalmus maximus muscle with the method of trypsin digestion. It has been subcultured more than 60 passages for over 150 days. The TMF cells were cultured in L-15 medium supplemented with HEPES, fetal bovine serum (FBS), GlutaMAX, and basic fibroblast growth factor (bFGF). The optimal ...
DOI:
10.1007/s10695-019-00628-3

5. Involvement and expression of growth hormone/insulin-like growth factor member mRNAs in the ovarian development of turbot (Scophthalmus maximus)

Author:
Jia, Yudong; Jing, Qiqi; Gao, Yunhong; Huang, Bin
Source:
Fish physiology and biochemistry 2019 v.45 no.3 pp. 955-964
ISSN:
0920-1742
Subject:
turbot,   etc   ; Scophthalmus maximus; abnormal development; animal ovaries; animal reproduction; binding proteins; breeding stock; gene expression; gonadosomatic index; hepatosomatic index; hormone receptors; insulin-like growth factor I; insulin-like growth factor I receptor; liver; messenger RNA; migratory behavior; ovarian development; somatotropin; vitellogenesis; vitellogenin;     Show all 20 Subjects
Abstract:
... Accumulating evidence suggests that the growth hormone (GH)/insulin-like growth factor (IGF) system participates in fish reproduction. To understand the physiological functions of the GH/IGF system, the mRNA expression profiles of all known members of the GH/IGF system, including hepatic and ovarian gh, GH receptor (ghr), IGFs (igf-i, igf-ii), IGF-I receptor (igf-ir) and IGF binding protein (igfbp ...
DOI:
10.1007/s10695-018-0604-z

6. Progestin is important for testicular development of male turbot (Scophthalmus maximus) during the annual reproductive cycle through functionally distinct progestin receptors

Author:
Feng, Chengcheng; Xu, Shihong; Liu, Yifan; Wang, Yanfeng; Wang, Wenqi; Yang, Jingkun; Zhao, Chunyan; Liu, Qinghua; Li, Jun
Source:
Fish physiology and biochemistry 2018 v.44 no.1 pp. 35-48
ISSN:
0920-1742
Subject:
turbot,   etc   ; Scophthalmus maximus; blood serum; breeding season; males; progesterone; progesterone receptors; seasonal variation; sperm motility; spermatids; spermatocytes; spermatozoa; spermiation; spermiogenesis; steroid hormones; testes; testicular development;     Show all 17 Subjects
Abstract:
... In teleost, sex steroid hormones are critical for reproduction. Progestin is known to promote spermiation. To further understand the functions of progestin via its receptors during the annual reproductive cycle in male turbot (Scophthalmus maximus), we observed testicular development, quantified several sex steroid hormones, detected the expression of progestin receptors, and measured various sper ...
DOI:
10.1007/s10695-017-0411-y

7. Gonadogenesis analysis and sex differentiation in cultured turbot (Scophthalmus maximus)

Author:
Zhao, Chunyan; Xu, Shihong; Liu, Yifan; Wang, Yanfeng; Liu, Qinghua; Li, Jun
Source:
Fish physiology and biochemistry 2017 v.43 no.1 pp. 265-278
ISSN:
0920-1742
Subject:
turbot,   etc   ; Scophthalmus maximus; animal ovaries; farmed fish; germ cells; histology; mesentery; mitosis; sexual dimorphism; sexual maturity; somatic cells; testes;     Show all 12 Subjects
Abstract:
... As a flatfish, the turbot (Scophthalmus maximus) is one of the most important farmed fish species with great commercial value, which has a strong sexual dimorphism on growth rate and sexual maturity. In this study, using histology, the basic information on proliferation and migration of germ cells and early gonadal development during sex differentiation in turbot were described in detail. There we ...
DOI:
10.1007/s10695-016-0284-5

8. Effect of the early temperature on the growth of larvae and postlarvae turbot, Scophthalmus maximus L.: muscle structural and ultrastructural study

Author:
Ayala, María D.; Martínez, Juan M.; Hernández-Urcera, Jorge; Cal, Rosa
Source:
Fish physiology and biochemistry 2016 v.42 no.3 pp. 1027-1042
ISSN:
0920-1742
Subject:
turbot,   etc   ; Scophthalmus maximus; ambient temperature; body length; hyperplasia; larvae; metamorphosis; muscles; postlarvae;     Show all 9 Subjects
Abstract:
... Turbot specimens were kept at three temperatures (T ₛ): warm (W) (21–22 °C), ambient (A) (17–18 °C) and cold (C) (13–14 °C) during the larval and early postlarval stages. At 90 days posthatching (dph), all of them were transferred to ambient T until 190 dph. At 2–3 dph, the specimens showed a monolayer of red muscle and immature white fibres; external or dermomyotome cells (presumptive myogenic ce ...
DOI:
10.1007/s10695-015-0194-y

9. Influence of immunostimulant polysaccharides, nucleic acids, and Bacillus strains on the innate immune and acute stress response in turbots (Scophthalmus maximus) fed soy bean- and wheat-based diets

Author:
Fuchs, V. I.; Schmidt, J.; Slater, M. J.; Buck, B. H.; Steinhagen, D.
Source:
Fish physiology and biochemistry 2017 v.43 no.6 pp. 1501-1515
ISSN:
0920-1742
Subject:
turbot,   etc   ; RNA; Scophthalmus maximus; active ingredients; alginates; aquaculture; beta-glucans; blood glucose; blood proteins; cholesterol; cortisol; diet; fish meal; glucose; hematologic tests; immune response; immunostimulants; innate immunity; juveniles; lysozyme; netting; neutrophils; nucleotides; oligosaccharides; reactive oxygen species; stress response; triacylglycerols; yeasts;     Show all 28 Subjects
Abstract:
... Immunostimulants are widely applied in aquaculture practice and may have beneficial effects on the immune system and physical functions allowing higher tolerance to stress. In the current study, the impact of four (i–iv) dietary active ingredients on the immune and stress response of turbot was examined in two experiments (I and II). A basal low fish meal (FM; 32%) diet was formulated and suppleme ...
DOI:
10.1007/s10695-017-0388-6

10. Leptin and its receptor in turbot Scophthalmus maximus: cloning, characterization and expression response to ratios of dietary carbohydrate–lipid

Author:
Han, Dongdong; Miao, Huijun; Nie, Qin; Miao, Shuyan; Zhang, Qin; Zhang, Wenbing; Mai, Kangsen
Source:
Fish physiology and biochemistry 2016 v.42 no.6 pp. 1665-1679
ISSN:
0920-1742
Subject:
turbot,   etc   ; Scophthalmus maximus; amino acid sequences; amino acids; brain; complementary DNA; diet; eyes; gene expression; grouper; humans; kidneys; leptin; liver; messenger RNA; nucleotide sequences; tissue distribution;     Show all 17 Subjects
Abstract:
... In the present study, the full-length cDNA sequences of leptin (LEP) and its receptor (LEPR) from turbot Scophthalmus maximus were cloned. The cDNA of tLEP was 1126 bp in length encoding 157 amino acids. The amino acid sequence shared low identity with human LEP (18.8 %), but the three-dimensional structures of these two LEPs were strongly conserved. The deduced 1173-amino acid sequence of tLEPR w ...
DOI:
10.1007/s10695-016-0248-9

11. Molecular characterization and expression analysis of AMPK α subunit isoform genes from Scophthalmus maximus responding to salinity stress

Author:
Zeng, Lin; Liu, Bin; Wu, Chang-Wen; Lei, Ji-Lin; Xu, Mei-Ying; Zhu, Ai-Yi; Zhang, Jian-She; Hong, Wan-Shu
Source:
Fish physiology and biochemistry 2016 v.42 no.6 pp. 1595-1607
ISSN:
0920-1742
Subject:
turbot,   etc   ; AMP-activated protein kinase; Scophthalmus maximus; amino acids; complementary DNA; energy balance; gene expression; genes; juveniles; messenger RNA; muscles; peptides; phylogeny; salinity; salt stress; sodium-potassium-exchanging ATPase; stress response; tissue distribution; tissues;     Show all 19 Subjects
Abstract:
... AMP-activated protein kinase (AMPK) is a highly conserved and multi-functional protein kinase that plays important roles in both intracellular energy balance and cellular stress response. In the present study, molecular characterization, tissue distribution and gene expression levels of the AMPK α1 and α2 genes from turbot (Scophthalmus maximus) under salinity stress are described. The complete co ...
DOI:
10.1007/s10695-016-0243-1

12. Chemokine receptor CXCR3 in turbot (Scophthalmus maximus): cloning, characterization and its responses to lipopolysaccharide

Author:
Chen, Yadong; Zhou, Shuhong; Jiang, Zhiqiang; Wang, Xiuli; Liu, Yang
Source:
Fish physiology and biochemistry 2016 v.42 no.2 pp. 659-671
ISSN:
0920-1742
Subject:
turbot,   etc   ; CXCR3 receptor; CXCR5 receptor; Oncorhynchus mykiss; Scophthalmus maximus; amino acids; blood; chemokines; complementary DNA; gene expression; genes; immune response; kidneys; lipopolysaccharides; liver; molecular cloning; phylogeny; quantitative polymerase chain reaction; spleen;     Show all 19 Subjects
Abstract:
... Chemokine (C-X-C motif) receptor 3, a member of the G protein-coupled receptors superfamily, regulates the responses of many immune responses. In this experiment, we cloned and characterized the cDNA of CXCR3 in Scophthalmus maximus (turbot). A 5′-UTR of 216-bp, a 259-bp 3′-UTR with a poly (A) tail and a 1089-bp CDS encoding 362 amino acids form the cDNA of CXCR3, which is 1564-bp long. Phylogenet ...
DOI:
10.1007/s10695-015-0167-1

13. Molecular characterization of the cathepsin B of turbot (Scophthalmus maximus)

Author:
Zhou, Ze-jun; Qiu, Reng; Zhang, Jian
Source:
Fish physiology and biochemistry 2015 v.41 no.2 pp. 473-483
ISSN:
0920-1742
Subject:
turbot,   etc   ; Escherichia coli; Scophthalmus maximus; active sites; amino acids; bacterial infections; bioactive properties; cathepsin B; enzyme activity; immune response; mutants; mutation; pH; proteins; tissues;     Show all 15 Subjects
Abstract:
... Cathepsin B is an enzymatic protein belonging to the peptidase C1 family. It is involved in diverse physiological and pathological functions that include immune response. In this study, we identified and characterized a cathepsin B homolog (SmCatB) from turbot (Scophthalmus maximus). SmCatB is composed of 330 amino acid residues and possesses typical domain architecture of cathepsin B, which conta ...
DOI:
10.1007/s10695-014-9998-4

14. Biochemical composition and quality of turbot (Scophthalmus maximus) eggs throughout the reproductive season

Author:
Jia, Yudong; Meng, Zhen; Liu, Xinfu; Lei, Jilin
Source:
Fish physiology and biochemistry 2014 v.40 no.4 pp. 1093-1104
ISSN:
0920-1742
Subject:
turbot,   etc   ; Scophthalmus maximus; alanine; aspartic acid; breeding stock; chemical composition; egg quality; eggs; fatty acids; glutamic acid; hatching; isoleucine; larvae; leucine; lysine; methionine; serine; valine; viability;     Show all 19 Subjects
Abstract:
... The present study investigated the biochemical composition and quality of turbot (Scophthalmus maximus) eggs throughout the reproductive season. Results showed that the fertilization, hatching and egg floating rates were variable throughout the reproductive season, with the highest values recorded during the mid-season. Meanwhile, positive correlations were found between fertilization, hatching ra ...
DOI:
10.1007/s10695-014-9908-9
PubMed:
24415175

15. Effects of dietary glucose and dextrin on activity and gene expression of glucokinase and fructose-1,6-bisphosphatase in liver of turbot Scophthalmus maximus

Author:
Nie, Qin; Miao, Huijun; Miao, Shuyan; Zhou, Huihui; Zhang, Yanjiao; Zhang, Wenbing; Mai, Kangsen
Source:
Fish physiology and biochemistry 2015 v.41 no.3 pp. 819-832
ISSN:
0920-1742
Subject:
turbot,   etc   ; Scophthalmus maximus; amino acids; brain; complementary DNA; dextrins; dietary carbohydrate; gene expression; genes; glucokinase; glucose; glycolysis; heart; intestines; kidneys; liver; messenger RNA; muscles; open reading frames; rapid amplification of cDNA ends;     Show all 20 Subjects
Abstract:
... Glucokinase (GK) and fructose-1,6-bisphosphatase (FBPase) play crucial role in glucose metabolism. In the present study, the cDNA encoding GK and FBPase was cloned from the liver of turbot Scophthalmus maximus by rapid amplification of cDNA end technique. Effects of dietary glucose and dextrin on the activities and gene expressions of these two enzymes were also studied. Results showed that the fu ...
DOI:
10.1007/s10695-015-0049-6

16. Molecular characterization and quantification of the follicle-stimulating hormone receptor in turbot (Scophthalmus maximus)

Author:
Jia, Yudong; Sun, Ai; Meng, Zhen; Liu, Baoliang; Lei, Jilin
Source:
Fish physiology and biochemistry 2016 v.42 no.1 pp. 179-191
ISSN:
0920-1742
Subject:
turbot,   etc   ; Hippoglossus hippoglossus; Scophthalmus maximus; abnormal development; amino acids; brain; breeding stock; complementary DNA; eyes; females; follicle-stimulating hormone; follicle-stimulating hormone receptors; genes; gills; glycoproteins; heart; intestines; kidneys; luteinizing hormone; messenger RNA; migratory behavior; molecular cloning; muscles; oocytes; open reading frames; ovarian development; sequence analysis; sheep; signal peptide; spleen; stomach; vitellogenesis;     Show all 32 Subjects
Abstract:
... Molecular cloning, characterization, and functional analysis of follicle-stimulating hormone receptor (FSHR) in female turbot (Scophthalmus maximus) were evaluated. Results showed that the full-length FSHR cDNA was 3824 bp long and contained a 2202 bp open reading frame that encoded a mature protein of 733 amino acids (aa) and a signal peptide of 18 aa. Multiple sequence analyses showed that turbo ...
DOI:
10.1007/s10695-015-0128-8

17. Changes in protein composition of epidermal mucus in turbot Scophthalmus maximus (L.) under high water temperature

Author:
Ai-Jun, Ma; Zhi-hui, Huang; Xin-An, Wang
Source:
Fish physiology and biochemistry 2013 v.39 no.6 pp. 1411-1418
ISSN:
0920-1742
Subject:
turbot,   etc   ; immunity; polypeptides; two-dimensional gel electrophoresis; proteome; aquaculture; biomarkers; lectins; Scophthalmus maximus; genotype; water temperature; mucus; databases; protein composition; prediction;     Show all 15 Subjects
Abstract:
... To explore the skin mucous of juvenile turbot (Scophthalmus maximus) protein under thermo-treatment in different temperatures (20, 23, 25, and 27 °C), the corresponding proteome maps were constructed by two-dimensional gel electrophoresis (2-DE), from which the peptide mass map with MALDI-TOF-TOF was obtained, and a novel protein of polypeptide was identified by database retrieval. Results show th ...
DOI:
10.1007/s10695-013-9795-5
PubMed:
23543157

18. Molecular cloning, characterization, and expression analysis of luteinizing hormone receptor gene in turbot (Scophthalmus maximus)

Author:
Jia, Yudong; Meng, Zhen; Niu, Huaxin; Hu, Peng; Lei, Jilin
Source:
Fish physiology and biochemistry 2014 v.40 no.6 pp. 1639-1650
ISSN:
0920-1742
Subject:
turbot,   etc   ; Hippoglossus hippoglossus; Scophthalmus maximus; abnormal development; amino acids; animal ovaries; complementary DNA; genes; glycoproteins; luteinizing hormone receptors; messenger RNA; migratory behavior; molecular cloning; oocytes; open reading frames; ovarian development; ovulation;     Show all 17 Subjects
Abstract:
... The luteinizing hormone receptor (LHR) plays a crucial role in female reproduction. In the present study, full-length sequence coding for the LHR was obtained from female turbot (Scophthalmus maximus) by homology cloning and a strategy based on rapid amplification of cDNA end-polymerase chain reaction. The full-length LHR cDNA was 3,184 bp long and contained a 2,058-bp open reading frame which enc ...
DOI:
10.1007/s10695-014-9954-3
PubMed:
24965493

19. Digestive enzyme activities of turbot (Scophthalmus maximus L.) during early developmental stages under culture condition

Author:
Tong, X. H.; Xu, S. H.; Liu, Q. H.; Li, J.; Xiao, Z. Z.; Ma, D. Y.
Source:
Fish physiology and biochemistry 2012 v.38 no.3 pp. 715-724
ISSN:
0920-1742
Subject:
turbot,   etc   ; leucyl aminopeptidase; diet; foods; membrane alanyl aminopeptidase; trypsin; carbohydrates; early development; microvilli; digestion; Scophthalmus maximus; stomach; larvae; enzyme activity; alkaline phosphatase; pepsin;     Show all 16 Subjects
Abstract:
... Digestive enzyme activities were analysed in turbot (Scophthalmus maximus) from hatching until 60 days after hatching (DAH). Trypsin sharply increased to the climax at 17 DAH and decreased until 31 DAH followed by a stable level thereafter. Amylase was determined at 4 DAH, reached the maximum value at 19 DAH and declined sharply to 39 DAH and remained at a low level thereafter, suggesting the carb ...
DOI:
10.1007/s10695-011-9553-5
PubMed:
21938391

20. Safeguarding the welfare of farmed fish at harvest

Author:
Lines, J. A.; Spence, J.
Source:
Fish physiology and biochemistry 2012 v.38 no.1 pp. 153-162
ISSN:
0920-1742
Subject:
turbot,   etc   ; fish culture; harvesting; trout; eel; fish kills; bream; Pangasius; animal welfare; carp; farming systems; tuna; methodology; halibut; catfish; farmed animal species; salmon; cod (fish); bass;     Show all 19 Subjects
Abstract:
... Fish welfare at harvest is easily compromised by poor choice of handling and slaughter methods, lack of attention to detail and by unnecessary adherence to fish farming traditions. The harvest process comprises fasting the fish to empty the gut, crowding the fish, gathering and moving the fish using brails, fish pumps, and sometimes also road or boat transport and finally stunning and killing the ...
DOI:
10.1007/s10695-011-9561-5
PubMed:
21989953