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- Nakayasu, Chihaya, et al. Show all 10 Authors
- Fisheries science 2015 v.81 no.5 pp. 907-914
- Seriola quinqueradiata; Vibrio anguillarum; gene expression regulation; genes; high-throughput nucleotide sequencing; interleukin-10; interleukin-1beta; interleukin-8; kidneys; pathogens; polyinosinic-polycytidylic acid; quantitative polymerase chain reaction; tumor necrosis factor-alpha; vaccination
- ... Partial sequences of cytokine genes IFNγ, IL-1β, IL-8, IL-10, and TNFα were identified from next-generation sequencing data for three amberjack species: Seriola quinqueradiata, S. lalandi and S. dumerili. The cytokine genes had more than 90 % nucleotide identity among the three species. Quantitative PCR (qPCR) primers were designed from the consensus sequences to detect cytokine genes. The specifi ...