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- Chua, Trina; Bhagwat, Arvind A.
- Food analytical methods 2009 v.2 no.2 pp. 96
- produce; raw fruit; raw vegetables; fresh-cut foods; microbial detection; rapid methods; bacterial contamination; food pathogens; Salmonella; Listeria monocytogenes; polymerase chain reaction; isolation; food safety; food microbiology; enrichment culture
- ... DNA isolation procedures significantly influence the outcome of PCR-based detection of human pathogens. Unlike clinical samples, DNA isolation from food samples, particularly from fresh and fresh-cut produce has remained a formidable task and has hampered the sensitivity and accuracy of molecular methods. We utilized a commercially available filter-based DNA isolation method (FTA) in conjunction w ...