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- Guo, Yongpeng, et al. Show all 6 Authors
- Food chemistry 2020 v.325 pp. 126877
- Bacillus licheniformis; Escherichia coli; aflatoxin B1; apoptosis; cell viability; computer simulation; enzyme activity; hepatocytes; humans; hydrogen bonding; laccase; van der Waals forces
- ... In the present study, the CotA protein from Bacillus licheniformis ANSB821 was cloned and expressed in Escherichia coli. Apart from the laccase activities, we found that the recombinant CotA could effectively oxidize aflatoxin B₁ in the absence of redox mediators. The Kₘ, Kcₐₜ and Vₘₐₓ values of the recombinant CotA towards aflatoxin B₁ were 60.62 μM, 0.03 s⁻¹ and 10.08 μg min⁻¹ mg⁻¹, respectively ...