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1. Development and application of a loop-mediated isothermal amplification assay on rapid and sensitive detection of rotavirus in fecal samples and artificially seeded oysters

Author:
Xue, Liang,   et al.   ; Kou, Xiaoxia; Fan, Hongying; Wu, Qingping; Zhang, Jumei;     Show all 5 Authors
Source:
Food control 2014 v.41 pp. 151-157
ISSN:
0956-7135
Subject:
RNA; Rotavirus; betaine; color; cross reaction; detection limit; feces; gel electrophoresis; genome; magnesium sulfate; monitoring; oysters; reverse transcription loop-mediated isothermal amplification; risk assessment; turbidity
Abstract:
... A reverse transcription-loop-mediated isothermal amplification (RT-LAMP) assay was developed for detection of rotavirus. Four primers were designed to recognize six distinct regions based on a highly conserved sequence in the VP7 region of the rotavirus genome. The optimal conditions for the LAMP assay were determined to 62 °C for 60 min with 8.0 mM MgSO4, 0.2 M betaine, 0.8 mM dNTPs, 0.2 μM each ...
DOI:
10.1016/j.foodcont.2014.01.013