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... The presence of enteric viruses on fresh berries poses a health risk to consumers. After berries are picked, a washing step can reduce microbial counts on fruit surfaces to increase their safety. This study compared the effectiveness of tap water, commercial solutions of fruit and vegetable sanitizer composed of peroxyacetic acid and hydrogen peroxide (PAA) and neutral electrolyzed oxidizing water ...
... Human norovirus is one of the potential foodborne pathogens causing acute gastroenteritis, diarrhea, and food poisoning. For norovirus detection, we developed the norovirus specifically capturable peptides functionalized gold nanoparticles (AuNP) decorated tungsten disulfide nanoflower (WS₂NF/AuNP). Based on the 3D-structured WS₂NF acting as a supporting material, AuNP were immobilized on its surf ...
... A reverse transcription-loop-mediated isothermal amplification (RT-LAMP) assay was developed for detection of rotavirus. Four primers were designed to recognize six distinct regions based on a highly conserved sequence in the VP7 region of the rotavirus genome. The optimal conditions for the LAMP assay were determined to 62 °C for 60 min with 8.0 mM MgSO4, 0.2 M betaine, 0.8 mM dNTPs, 0.2 μM each ...
... Foodborne viruses such as norovirus (NoV), hepatitis A virus (HAV), hepatitis E virus (HEV), and rotavirus (RoV) are transmitted through water and food contaminated with stool. The purpose of this study was to examine the prevalence of foodborne viruses in shellfish collected in South Korea using real-time RT-PCR. Virus was eluted from the stomach and the digestive diverticula of 152 shellfish (51 ...
... This study’s aim was to develop a specific and sensitive reverse transcription polymerase chain reaction enzyme-linked immunosorbent assay (RT-PCR-ELISA) for hepatitis A virus (HAV). Three concentration methods were compared using samples from cabbage, lettuce, and sesame leaves that were artificially contaminated with HAV. The specificity of the assay was tested against human norovirus, hepatitis ...