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- Xue, Liang, et al. Show all 14 Authors
- Food control 2020 v.111 pp. 107032
- Norovirus; RNA-directed DNA polymerase; detection limit; foodborne illness; gastroenteritis; markets; oysters; proteinases; quantitative polymerase chain reaction; reverse transcriptase polymerase chain reaction
- ... Noroviruses are the primary cause of gastroenteritis and foodborne diseases, affecting millions of individuals annually worldwide. Oysters are frequently associated with norovirus outbreaks. Hence, inexpensive and simple norovirus detection methods are important to detect and contain foodborne illness outbreaks. The objective of this study is to develop a high-efficient concentrated pretreatment m ...
- Xue, Liang, et al. Show all 5 Authors
- Food control 2014 v.41 pp. 151-157
- RNA; Rotavirus; betaine; color; cross reaction; detection limit; feces; gel electrophoresis; genome; magnesium sulfate; monitoring; oysters; reverse transcription loop-mediated isothermal amplification; risk assessment; turbidity
- ... A reverse transcription-loop-mediated isothermal amplification (RT-LAMP) assay was developed for detection of rotavirus. Four primers were designed to recognize six distinct regions based on a highly conserved sequence in the VP7 region of the rotavirus genome. The optimal conditions for the LAMP assay were determined to 62 °C for 60 min with 8.0 mM MgSO4, 0.2 M betaine, 0.8 mM dNTPs, 0.2 μM each ...