Author: "Xue, Liang" / Journal: Food control / Subject: 4 selected

Main content area

Author:: Xue, Liang, et al. ; Kou, Xiaoxia; Fan, Hongying; Wu, Qingping; Zhang, Jumei; Show all 5 Authors
Source:: Food control 2014 v.41 pp. 151-157
ISSN:: 0956-7135
Subject:: RNA; Rotavirus; betaine; color; cross reaction; detection limit; feces; gel electrophoresis; genome; magnesium sulfate; monitoring; oysters; reverse transcription loop-mediated isothermal amplification; risk assessment; turbidity
Abstract:: ... A reverse transcription-loop-mediated isothermal amplification (RT-LAMP) assay was developed for detection of rotavirus. Four primers were designed to recognize six distinct regions based on a highly conserved sequence in the VP7 region of the rotavirus genome. The optimal conditions for the LAMP assay were determined to 62 °C for 60 min with 8.0 mM MgSO4, 0.2 M betaine, 0.8 mM dNTPs, 0.2 μM each ...
DOI:: 10.1016/j.foodcont.2014.01.013
: https://dx.doi.org/10.1016/j.foodcont.2014.01.013