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- Ginart, S.; Caputo, M.; Corach, D.; Sala, A.
- Forensic science international 2019 v.295 pp. 1-7
- DNA; analytical kits; cost effectiveness; crime; females; humans; males; melting; quantitative polymerase chain reaction
- ... We developed a q-PCR technique that simultaneously evaluates the extent of degradation and determines the gender of a human DNA donor. QYDEG HRM is a triplex real-time PCR whose products are analysed by high-resolution melting (HRM). The system produces three amplicons: (1) transducin (beta)-like 1, Y-linked (TBL1Y) (84bp); (2) large-target sequence (DGlt) (244bp); and (3) small-target sequence (D ...
- Falkena, Kim; Hoveling, Richelle J.M.; van Weert, Angela; Lambrechts, Saskia A.G.; van Leeuwen, Ton G.; Aalders, Maurice C.G.; van Dam, Annemieke
- Forensic science international 2019 v.295 pp. 128-136
- DNA; biocompatible materials; crime; forensic sciences; microsatellite repeats; prediction; quantitative polymerase chain reaction
- ... During criminal investigations trace DNA samples, including fingermarks, are submitted to laboratories for short tandem repeat (STR) analysis. For most common STR analysis systems a minimum amount of input DNA is required. Upon intake by the forensic laboratory the DNA concentration is estimated using quantitative polymerase chain reaction (qPCR) analysis after which most fingermarks are excluded. ...