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- Ginart, S.; Caputo, M.; Corach, D.; Sala, A.
- Forensic science international 2019 v.295 pp. 1-7
- DNA; analytical kits; cost effectiveness; crime; females; humans; males; melting; quantitative polymerase chain reaction
- ... We developed a q-PCR technique that simultaneously evaluates the extent of degradation and determines the gender of a human DNA donor. QYDEG HRM is a triplex real-time PCR whose products are analysed by high-resolution melting (HRM). The system produces three amplicons: (1) transducin (beta)-like 1, Y-linked (TBL1Y) (84bp); (2) large-target sequence (DGlt) (244bp); and (3) small-target sequence (D ...