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1. Human DNA degradation assessment and male DNA detection by quantitative-PCR followed by high-resolution melting analysis

Author:
Ginart, S.; Caputo, M.; Corach, D.; Sala, A.
Source:
Forensic science international 2019 v.295 pp. 1-7
ISSN:
0379-0738
Subject:
DNA; analytical kits; cost effectiveness; crime; females; humans; males; melting; quantitative polymerase chain reaction
Abstract:
... We developed a q-PCR technique that simultaneously evaluates the extent of degradation and determines the gender of a human DNA donor. QYDEG HRM is a triplex real-time PCR whose products are analysed by high-resolution melting (HRM). The system produces three amplicons: (1) transducin (beta)-like 1, Y-linked (TBL1Y) (84bp); (2) large-target sequence (DGlt) (244bp); and (3) small-target sequence (D ...
DOI:
10.1016/j.forsciint.2018.11.013

2. Prediction of DNA concentration in fingermarks using autofluorescence properties

Author:
Falkena, Kim; Hoveling, Richelle J.M.; van Weert, Angela; Lambrechts, Saskia A.G.; van Leeuwen, Ton G.; Aalders, Maurice C.G.; van Dam, Annemieke
Source:
Forensic science international 2019 v.295 pp. 128-136
ISSN:
0379-0738
Subject:
DNA; biocompatible materials; crime; forensic sciences; microsatellite repeats; prediction; quantitative polymerase chain reaction
Abstract:
... During criminal investigations trace DNA samples, including fingermarks, are submitted to laboratories for short tandem repeat (STR) analysis. For most common STR analysis systems a minimum amount of input DNA is required. Upon intake by the forensic laboratory the DNA concentration is estimated using quantitative polymerase chain reaction (qPCR) analysis after which most fingermarks are excluded. ...
DOI:
10.1016/j.forsciint.2018.12.005