PubAg

Main content area

Search

39  Search Results

« Previous | 1 - 20 of 39 |
Number of results to display per page

Search Results

1. A panel of seven-miRNA signature in plasma as potential biomarker for colorectal cancer diagnosis

Author:
Zhang, Huo; Zhu, Mingxia; Shan, Xia; Zhou, Xin; Wang, Tongshan; Zhang, Jinying; Tao, Jinsong; Cheng, Wenfang; Chen, Gang; Li, Jian; Liu, Ping; Wang, Qiang; Zhu, Wei
Source:
Gene 2019 v.687 pp. 246-254
ISSN:
0378-1119
Subject:
biomarkers; colorectal neoplasms; exosomes; gene expression regulation; microRNA; patients; quantitative polymerase chain reaction; reverse transcriptase polymerase chain reaction; screening; tissues
Abstract:
... Colorectal cancer (CRC) has been one of the most commonly diagnosed cancers in global. The differential expression profiles of microRNAs (miRNAs) in CRC plasma of patients have the potential to serve as a diagnostic biomarker. We conducted a four-stage study to identify the potential plasma miRNAs for CRC detection. In the initial screening phase, Exiqon panel (miRCURY-Ready-to-Use-PCR-Human-panel ...
DOI:
10.1016/j.gene.2018.11.055

2. Aquaporins as a link between water relations and photosynthetic pathway in abiotic stress tolerance in plants

Author:
Pawłowicz, Izabela; Masajada, Katarzyna
Source:
Gene 2019 v.687 pp. 166-172
ISSN:
0378-1119
Subject:
abiotic stress; aquaporins; carbon dioxide; leaves; mesophyll; permeability; photosynthesis; plant organs; roots; solutes; stress tolerance
Abstract:
... Plant aquaporins constitute a large family of proteins involved in facilitating the transport of water and small neutral molecules across biological membranes. In higher plants they are divided into several sub-families, depending on membrane-type localization and permeability to specific solutes. They are abundantly expressed in the majority of plant organs and tissues, and play a function in pri ...
DOI:
10.1016/j.gene.2018.11.031

3. Associations between HMGB1 gene polymorphisms and susceptibility and clinical outcomes in Chinese Han sepsis patients

Author:
Qiu, Pingfeng; Wang, Licheng; Ni, Jianming; Zhang, Yunlong
Source:
Gene 2019 v.687 pp. 23-29
ISSN:
0378-1119
Subject:
Chinese people; alleles; blood serum; enzyme-linked immunosorbent assay; genetic variation; genotype; loci; mutants; nucleoproteins; patients; risk factors; sepsis (infection); single nucleotide polymorphism; survival rate
Abstract:
... High mobility group box 1 protein (HMGB1) is an important late inflammatory mediator in the body. In recent years, studies have found that it plays an important pathogenic role in various diseases such as sepsis. However, it is unclear whether the genetic variation of the HMGB1 gene is related to the susceptibility to sepsis. This study investigated the relationship between susceptibility and outc ...
DOI:
10.1016/j.gene.2018.11.027

4. Co-expression profiling of plasma miRNAs and long noncoding RNAs in gastric cancer patients

Author:
Ghaedi, Hamid; Mozaffari, Mohammad Amin Nazer; Salehi, Zaker; Ghasemi, Hassan; Zadian, Seyed Sajjad; Alipoor, Sadegh; Hadianpour, Shiva; Alipoor, Behnam
Source:
Gene 2019 v.687 pp. 135-142
ISSN:
0378-1119
Subject:
case-control studies; microRNA; non-coding RNA; pathogenesis; patients; quantitative polymerase chain reaction; stomach neoplasms
Abstract:
... The recent researches indicate that differential non-coding RNAs expression signatures could be associated with the pathogenesis of gastric cancer (GC). However, there are few studies focused on lncRNA-miRNAs co-expression profiling in GC patients. Therefore, in the present study the expression of H19 and MEG3 and their related miRNAs including miR-148a-3p, miR-181a-5p, miR-675-5p and miR-141-3p w ...
DOI:
10.1016/j.gene.2018.11.034

5. Dickkopf-1 reduces hypertrophic changes in human chondrocytes derived from bone marrow stem cells

Author:
Rojas, Andrea; Mardones, Rodrigo; Pritzker, Kenneth; van Wijnen, Andre J.; Galindo, Mario A.; Las Heras, Facundo
Source:
Gene 2019 v.687 pp. 228-237
ISSN:
0378-1119
Subject:
alkaline phosphatase; bone marrow; cartilage; cell culture; chondrocytes; collagen; genes; glycosaminoglycans; growth plate; humans; hypertrophy; mesenchymal stromal cells; muscles; phenotype; sequence analysis; stem cells; tissue engineering
Abstract:
... The in vitro process of chondrogenic differentiation of mesenchymal stem cells (MSCs) induces a pre-apoptotic hypertrophic phenotype, guided by the active status of the WNT/β‑catenin pathway. To achieve a stable chondrocyte phenotype for cartilage tissue engineering, it is necessary to gain a better understanding of specific genes that regulate the cartilage tissue phenotype. RNA sequencing (RNA-s ...
DOI:
10.1016/j.gene.2018.11.037

6. Dmrt1 directly regulates the transcription of the testis-biased Sox9b gene in Nile tilapia (Oreochromis niloticus)

Author:
Wei, Ling; Li, Xiaoyan; Li, Minghui; Tang, Yaohao; Wei, Jing; Wang, Deshou
Source:
Gene 2019 v.687 pp. 109-115
ISSN:
0378-1119
Subject:
Oreochromis niloticus; chromatin; gel electrophoresis; genes; luciferase; males; mammals; precipitin tests; regulatory sequences; sexual development; site-directed mutagenesis; testes; transcription (genetics); transcription factors; translation (genetics)
Abstract:
... The Nile tilapia Sox9b gene is characterized as a homolog of the mammalian Sox9 gene, which exhibits testis-biased expression and is involved in testis development. However, the transcriptional regulation of the Sox9b gene is poorly understood. In this study, we demonstrated that the male sex differentiation gene doublesex and mab-3 related transcription factor 1 (Dmrt1) was predominantly expresse ...
DOI:
10.1016/j.gene.2018.11.016

7. Effects of Paraoxonase-1 variants on course of severity and mortality of Crimean-Congo hemorrhagic fever

Author:
Karakus, Nevin; Yigit, Serbulent; Duygu, Fazilet; Barut, Sener; Rustemoglu, Aydin; Basol, Nursah
Source:
Gene 2019 v.687 pp. 188-192
ISSN:
0378-1119
Subject:
Crimean-Congo hemorrhagic fever; alleles; disease severity; fever; genotype; high density lipoprotein; mortality; oxidative stress; patients; polymerase chain reaction; restriction fragment length polymorphism; single nucleotide polymorphism; viruses
Abstract:
... Crimean-Congo hemorrhagic fever (CCHF) is an acute viral hemorrhagic fever caused by Crimean Congo hemorrhagic fever virus (CCHFV). Paraoxonase-1 (PON1) is a high density lipoprotein (HDL)-binding protein which defense the body against oxidative stress. To investigate the role of the PON1 gene in CCHF, we screened the genotypes of two single nucleotide polymorphisms (Q192R [rs662] and L55M [rs8545 ...
DOI:
10.1016/j.gene.2018.11.059

8. Evaluation of miRNA-9 and miRNA-34a as potential biomarkers for diagnosis of breast cancer in Iranian women

Author:
Orangi, Elham; Motovali-Bashi, Majid
Source:
Gene 2019 v.687 pp. 272-279
ISSN:
0378-1119
Subject:
analysis of variance; biomarkers; breast neoplasms; data analysis; microRNA; patients; reverse transcriptase polymerase chain reaction; t-test; tissues; women; women's health
Abstract:
... MicroRNAs are involved in diverse biological processes and their dysregulation is a common event in various diseases including breast cancer. Breast cancer is a major threat to women's health. This study was designed to examine the expression levels of miR-9 and miR-34a in breast tumor tissue samples and plasma of breast cancer patients, compare their expression pattern between tissue samples and ...
DOI:
10.1016/j.gene.2018.11.036

9. Expression profile analysis identifies the long non-coding RNA landscape and the potential carcinogenic functions of LINC00668 in laryngeal squamous cell carcinoma

Author:
Zhao, Lei; Cao, Huan; Chi, Weiwei; Meng, Wenxia; Cui, Weina; Guo, Wei; Wang, Baoshan
Source:
Gene 2019 v.687 pp. 47-55
ISSN:
0378-1119
Subject:
bioinformatics; carcinogenicity; cell proliferation; data collection; gene expression; gene expression regulation; genes; in vitro studies; landscapes; larynx; lymph nodes; messenger RNA; metastasis; non-coding RNA; quantitative polymerase chain reaction; reverse transcriptase polymerase chain reaction; screening; squamous cell carcinoma
Abstract:
... In order to explore the differentially expressed long non-coding RNAs (lncRNAs) in laryngeal squamous cell carcinoma (LSCC), the GSE84957 lncRNA expression profile was included in the present study through data mining in the National Center for Biotechnology Information/Gene Expression Omnibus (NCBI/GEO). Then, the differentially expressed genes (DEGs) of LSCC (1646 lncRNAs and 2713 mRNAs, fold ch ...
DOI:
10.1016/j.gene.2018.11.020

10. Genetic variations in inflammation-related genes and their influence on the susceptibility of pediatric acute lung injury in a Chinese population

Author:
Zhao, Xinqian; He, Jing; Xie, Guoyan; Xu, Song; Xie, Jun; Chen, Youyuan; Wu, Hui
Source:
Gene 2019 v.687 pp. 16-22
ISSN:
0378-1119
Subject:
adults; alleles; biomarkers; children; genetic variation; genotyping; heterozygosity; homozygosity; interleukin-10; interleukin-18; interleukin-6; lungs; pathogenesis; patients; regression analysis; restriction fragment length polymorphism; risk; tumor necrosis factors
Abstract:
... Acute lung injury (ALI) is a serious clinical syndrome that can cause respiratory failure and threaten the life of the patients. A biomarker that can predict the syndrome can contribute to a better clinical management of the patients. In adults, genetic polymorphisms in inflammatory-response genes have been shown to be a promising biomarker. However, the pathogenesis of ALI in adult is known to be ...
DOI:
10.1016/j.gene.2018.11.009

11. Genome-wide identification and characterization of the Dof gene family in cassava (Manihot esculenta)

Author:
Zou, Zhi; Zhu, Jiali; Zhang, Xicai
Source:
Gene 2019 v.687 pp. 298-307
ISSN:
0378-1119
Subject:
Manihot esculenta; cassava; chromosomes; genes; phylogeny; staple crops; tissues; transcription (genetics); transcription factors
Abstract:
... Dof proteins comprise a plant-specific transcription factor family involved in regulation of many plant processes. Despite their diverse roles, little information is available in cassava (Manihot esculenta, Euphorbiaceae), the sixth major staple crop in the world. This study presents the first genome-wide identification of Dof family genes in cassava, resulting in 45 family members that are uneven ...
DOI:
10.1016/j.gene.2018.11.053

12. High incidence of co-existing factors significantly modifying the phenotype in patients with Fabry disease

Author:
Koca, Serhat; Tümer, Leyla; Okur, İlyas; Erten, Yasemin; Bakkaloğlu, Sevcan; Biberoğlu, Gürsel; Kasapkara, Çiğdem; Küçükçongar, Aynur; Dalgıç, Buket; Oktar, Suna Özhan; Öner, Yusuf; Atalay, Tuba; Cemri, Mustafa; Çiftçi, Bahattin; Topçu, Burcu; Hasanoğlu, Alev; Ezgü, Fatih
Source:
Gene 2019 v.687 pp. 280-288
ISSN:
0378-1119
Subject:
Fabry disease; alpha-galactosidase; antibodies; celiac disease; cholesterol; disease course; females; fever; heterozygosity; homocysteine; image analysis; immunoglobulin M; lipoproteins; males; patients; phenotype; prothrombin; risk factors; signs and symptoms (animals and humans); Turkey (country)
Abstract:
... Fabry disease results from deficiency of the lysosomal enzyme alpha-galactosidase A.The families of 11 index cases were screened by enzyme and molecular assays. Further clinical and laboratory investigations were carried out in all cases.Including 33 new patients, a total of 28 females (Age 25,82 ± 12,1 Range 8–46) and 16 males (Age 24,56 ± 15,04 Range 2–48) were investigated. Ten different diseas ...
DOI:
10.1016/j.gene.2018.11.054

13. hnRNPDL extensively regulates transcription and alternative splicing

Author:
Li, Ruth Zhen; Hou, Jing; Wei, Yaxun; Luo, Xiaotian; Ye, Yali; Zhang, Yi
Source:
Gene 2019 v.687 pp. 125-134
ISSN:
0378-1119
Subject:
RNA-binding proteins; alternative splicing; apoptosis; cell movement; cell proliferation; females; gene expression; genes; messenger RNA; pregnancy; sequence analysis; signal transduction; transcription (genetics); transcriptome; tumor necrosis factors
Abstract:
... RNA binding proteins (RBPs) are key players of genome regulation. Here we report the transcriptome study of HnRNP D-Like protein, which belongs to the hnRNP family. We used RNA-seq to analyze the global transcript level and alternative splicing on hnRNPDL shRNA-treated cells and control. Sh-hnRNPDL extensively increased in the expression of genes involved in female pregnancy, cell apoptosis, cell ...
DOI:
10.1016/j.gene.2018.11.026

14. Identification and expression analysis of a novel miRNA derived from ERV-E1 LTR in Equus caballus

Author:
Jo, Ara; Lee, Hee-Eun; Kim, Heui-Soo
Source:
Gene 2019 v.687 pp. 238-245
ISSN:
0378-1119
Subject:
Retroviridae; binding sites; bioinformatics; databases; genome; horses; messenger RNA; microRNA; phylogeny; quantitative polymerase chain reaction; terminal repeat sequences; tissues
Abstract:
... Horses (Equus caballus) have been domesticated and bred to enhance speed, strength, and agility. Members of the Equus caballus Endogenous Retrovirus (EqERV) family affect several of these abilities in horses. EqERV elements have been integrated in the horse genome during evolution and generate repeat elements such as long terminal repeats (LTRs). LTR sequences are involved in retrovirus replicatio ...
DOI:
10.1016/j.gene.2018.11.043

15. Inactivation of SMARCA2 by promoter hypermethylation drives lung cancer development

Author:
Wu, Jixiang; He, Keshuai; Zhang, Yajun; Song, Jixiang; Shi, Zhan; Chen, Weiwei; Shao, Yongfeng
Source:
Gene 2019 v.687 pp. 193-199
ISSN:
0378-1119
Subject:
adenocarcinoma; carcinogenesis; chromatin; databases; gene expression; gene expression regulation; genomic islands; in vitro studies; lung neoplasms; lungs; messenger RNA; methylation; neoplasm cells; patients; tissues; transcription (genetics); tumor suppressor genes
Abstract:
... The SWI/SNF complex is a multimeric chromatin remodeling complex that has vital roles in regulating gene expression and cancer development. However, to date few studies have deeply explored the mechanism of SMARCA2 inactivation. We applied multi-omics analysis to explore the mechanism of SMARCA2 inactivation in The Cancer Genome Atlas (TCGA) database and performed the dCas9-DNMT3a system to evalua ...
DOI:
10.1016/j.gene.2018.11.032

16. Independent association of whole blood miR-328 expression and polymorphism at 3′UTR of the PAX6 gene with myopia

Author:
Kunceviciene, Edita; Liutkeviciene, Rasa; Budiene, Brigita; Sriubiene, Margarita; Smalinskiene, Alina
Source:
Gene 2019 v.687 pp. 151-155
ISSN:
0378-1119
Subject:
3' untranslated regions; DNA; alleles; blood cells; blood sampling; genotype; genotyping; homeotic genes; myopia; odds ratio; patients; quantitative polymerase chain reaction; risk; single nucleotide polymorphism
Abstract:
... This study aimed to find associations between miR-328 expression in whole blood, polymorphism at 3′UTR of the PAX6 gene (paired box homeotic gene 6) and myopia.We evaluated 451 individuals (142 individuals with low, 49 with moderate and 13 with high-degree myopia, and 247 healthy individuals). DNA and RNA were extracted from peripheral blood samples. Expression of miR-328 was assessed and genotypi ...
DOI:
10.1016/j.gene.2018.11.030

17. In-depth study of lin-28 suggests selectively conserved let-7 independent mechanism in Drosophila

Author:
Lee, Mihye; Nguyen, Thanh My Thi; Kim, Kiyoung
Source:
Gene 2019 v.687 pp. 64-72
ISSN:
0378-1119
Subject:
Drosophila; RNA-binding proteins; adults; biogenesis; death; embryogenesis; gene overexpression; humans; intestines; larvae; messenger RNA; muscles; mutants; oogenesis; pathogenesis; pupae; stem cells; translation (genetics)
Abstract:
... Lin-28 is a conserved RNA-binding protein that is involved in a wide range of developmental processes and pathogenesis. At the molecular level, Lin-28 blocks the maturation of let-7 and regulates translation of certain mRNA targets. In Drosophila, Lin-28 is reported to play a role in oogenesis, muscle formation, and the symmetric division of adult intestinal stem cells. In this study, we character ...
DOI:
10.1016/j.gene.2018.11.017

18. Insight into long noncoding competing endogenous RNA networks in hepatic fibrosis: The potential implications for mechanism and therapy

Author:
Dong, Ben-Sheng; Shi, Miao-Juan; Su, Shi-Bing; Zhang, Hui
Source:
Gene 2019 v.687 pp. 255-260
ISSN:
0378-1119
Subject:
DNA; liver cirrhosis; messenger RNA; microRNA; non-coding RNA; pathological processes and conditions; prognosis; proteins; therapeutics
Abstract:
... Mounting evidence has shown that long noncoding RNAs (lncRNAs) can function as competing endogenous RNAs (ceRNAs) in distinct physiological and pathological states. The activation of hepatic stellate cells (HSCs) is a critical event in the development of hepatic fibrosis (HF). LncRNAs have recently been revealed to be involved in HSC activation as ceRNAs. CeRNA analysis tremendously expands the fu ...
DOI:
10.1016/j.gene.2018.11.063

19. Investigation of the synergistic effects of paclitaxel and herbal substances and endemic plant extracts on cell cycle and apoptosis signal pathways in prostate cancer cell lines

Author:
Doğan Şiğva, Zeynep Özlem; Balci Okcanoğlu, Tuğçe; Biray Avci, Çığır; Yilmaz Süslüer, Sunde; Kayabaşi, Çağla; Turna, Burak; Dodurga, Yavuz; Nazli, Oktay; Gündüz, Cumhur
Source:
Gene 2019 v.687 pp. 261-271
ISSN:
0378-1119
Subject:
Alkanna; Phlomis; Rubia; Taxus brevifolia; active ingredients; anticarcinogenic activity; apoptosis; cell cycle checkpoints; cell lines; cyclins; drugs; gene expression; indigenous species; inhibitory concentration 50; neoplasm cells; paclitaxel; plant extracts; plants (botany); prostatic neoplasms; signal transduction; silymarin; synergism; Turkey (country)
Abstract:
... Paclitaxel, which isolated from Taxus brevifolia, is recently started to be used against prostate cancer treatment and it is a very effective compound against cancer. In this study, we aimed to test the synergistic effect of two plant active compounds (sulphoraphane (SFN) and silymarin (SILY)) and several endemic plant species from Turkey (such as Phlomis leucophracta, Rubia davisiana, Alkanna tin ...
DOI:
10.1016/j.gene.2018.11.049

20. Klf4 could be activated by Pou5f3/Stat3 to induce the expression of Nanog in Japanese flounder

Author:
Sun, Yan; Liu, Jinxiang; Wang, Bo; Liu, Xiumei; Du, Xinxin; Liu, Yuxiang; Zhang, Quanqi
Source:
Gene 2019 v.687 pp. 56-63
ISSN:
0378-1119
Subject:
Paralichthys olivaceus; animal ovaries; cell proliferation; complementary DNA; epithelial cells; epithelium; flounder; gastrulation; gene expression regulation; genes; gills; neoplasms; nucleotide sequences; oocytes; oogonia; phylogeny; spermatogonia; testes; transcription factors; zinc finger motif
Abstract:
... Krüppel-like factor 4 (Klf4) is a zinc finger protein transcription factor of the KLF family and plays an important role in various important biological process, including cell proliferation, differentiation, embryonic development, and cancer formation. In this study, the full length Klf4 cDNA sequence of Japanese flounder (Paralichthys olivaceus) was analyzed. Phylogenetic, gene structure, and sy ...
DOI:
10.1016/j.gene.2018.11.029