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- Canto-Canché, Blondy B., et al. Show all 6 Authors
- Gene 2016 v.593 no.1 pp. 154-161
- clones; databases; genes; genomic libraries; groundwater; hydrolysis; metagenomics; microorganisms; models; open reading frames; phenotype; phylogeny; proteinases; proteins; proteolysis; screening; sequence analysis; signal peptide; zymogens
- ... Metagenomics is a culture-independent technology that allows access to novel and potentially useful genetic resources from a wide range of unknown microorganisms. In this study, a fosmid metagenomic library of tropical underground water was constructed, and clones were functionally screened for extracellular proteolytic activity. One of the positive clones, containing a 41,614-bp insert, had two g ...