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- Jennings, W. Bryan; Ruschi, Piero A.; Ferraro, Gustavo; Quijada, Carla Christie; Silva-Malanski, Ana Cecilia Gomes; Prosdocimi, Francisco; Buckup, Paulo A.
- Genome 2019 v.62 no.2 pp. 77-83
- Cichliformes; Cyprinodontiformes; DNA barcoding; DNA primers; Gymnotiformes; Neotropics; Siluriformes; cytochrome-c oxidase; data quality; fauna; freshwater fish; mitochondria; tropical fish; South America
- ... Designing primers for DNA barcoding is a significant challenge for the rich Neotropical fish fauna, which is comprised of ∼6000 species. Previously, researchers required multiple pairs of PCR primers or primer cocktails to obtain standard COI (i.e., mitochondrial cytochrome c oxidase subunit I) barcode sequences from assemblages of freshwater fish in this region. To simplify DNA barcoding and meta ...
- Prisingkorn, Wassana; Jakovlić, Ivan; Yi, Shao-Kui; Deng, Fang-Yu; Zhao, Yu-Hua; Wang, Wei-Min
- Genome 2019 v.62 no.2 pp. 53-67
- Megalobrama amblycephala; aquaculture; biogenesis; carbohydrates; diet; energy; fish; fish health; fish meal; gene expression; gene expression regulation; genes; homeostasis; humans; lipids; liver; metabolic diseases; metabolism; mitochondria; models; neurodegenerative diseases; nutrient utilization; quantitative polymerase chain reaction; transcriptome
- ... Expensive and unsustainable fishmeal is increasingly being replaced with cheaper lipids and carbohydrates as sources of energy in aquaculture. Although it is known that the excess of lipids and carbohydrates has negative effects on nutrient utilization, growth, metabolic homeostasis, and health of fish, our current understanding of mechanisms behind these effects is limited. To improve the underst ...