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- Jennings, W. Bryan, et al. Show all 7 Authors
- Genome 2019 v.62 no.2 pp. 77-83
- Cichliformes; Cyprinodontiformes; DNA barcoding; DNA primers; Gymnotiformes; Neotropics; Siluriformes; cytochrome-c oxidase; data quality; fauna; freshwater fish; mitochondria; tropical fish; South America
- ... Designing primers for DNA barcoding is a significant challenge for the rich Neotropical fish fauna, which is comprised of ∼6000 species. Previously, researchers required multiple pairs of PCR primers or primer cocktails to obtain standard COI (i.e., mitochondrial cytochrome c oxidase subunit I) barcode sequences from assemblages of freshwater fish in this region. To simplify DNA barcoding and meta ...