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- Belle, Simon; Parent, Claire
- Geomicrobiology journal 2019 v.36 no.6 pp. 570-579
- DNA; air temperature; climate change; community structure; lakes; meteorological data; methane; methanotrophs; microbial communities; nitrogen; nutrients; organic matter; oxidation; quantitative polymerase chain reaction; sediments; temporal variation
- ... In this study, a quantitative PCR (qPCR) method was applied to amplify ancient DNA (aDNA) of different methane-oxidizing bacteria (MOB) types in lake sediments and to reconstruct microbial community dynamics over the last 1200 years. We also used reconstructions of in-lake nutrients concentrations, air temperature fluctuations, and sedimentary organic matter dynamics to study impacts of past envir ...