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You searched for: Journal Horticulture, environment and biotechnology Remove constraint Journal: Horticulture, environment and biotechnology Publication Year 2018 Remove constraint Publication Year: 2018 Source 2018 v.59 no.4 Remove constraint Source: 2018 v.59 no.4 Subject cultivars Remove constraint Subject: cultivars Subject Chinese cabbage Remove constraint Subject: Chinese cabbage
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1. Establishment of an efficient regeneration system using heading leaves of Chinese cabbage (Brassica rapa L.) and its application in genetic transformation

Author:
Liu, Weixin; Yang, Yingjie; Liu, Qianqian
Source:
Horticulture, environment and biotechnology 2018 v.59 no.4 pp. 583-596
ISSN:
2211-3452
Subject:
Brassica rapa; Chinese cabbage; Southern blotting; benzyladenine; cultivars; culture media; explants; genetic transformation; kanamycin; leaves; naphthaleneacetic acid; polymerase chain reaction; seeds; shoots; silver nitrate; temperature; wilting; China
Abstract:
... To preserve and propagate valuable Chinese cabbage (Brassica rapa L.) using mature vegetative organs without seeds, we established a high efficiency regeneration system from heading leaf explants and further explored its application in genetic transformation. Our results showed that maximum shoot regeneration of cultivars ‘Beijing New No. 3’ (88.46 ± 1.65%) and ‘Chengyangqing’ (73.77 ± 1.73%) were ...
DOI:
10.1007/s13580-018-0064-5