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You searched for: Journal In vitro cellular & developmental biology Remove constraint Journal: In vitro cellular & developmental biology Publication Year 2015 Remove constraint Publication Year: 2015 Source 2015 v.51 no.6 Remove constraint Source: 2015 v.51 no.6
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1. An efficient multiple shoot induction and genetic fidelity assessment of Exacum bicolor Roxb., an endemic and endangered medicinal plant Plant

Author:
Ashwini, Appaji Mahesh; Ramakrishnaiah, Hanumanthaiah; Manohar, Shirugumbi Hanamanthagouda; Majumdar, Mala
Source:
In vitro cellular & developmental biology 2015 v.51 no.6 pp. 659-668
ISSN:
1054-5476
Subject:
DNA; Exacum; agar; benzyladenine; cytokinins; explants; gene expression regulation; genetic variation; germplasm conservation; in vitro studies; indole butyric acid; isopentenyladenine; medicinal plants; microsatellite repeats; plantlets; random amplified polymorphic DNA technique; shoots; sucrose; survival rate; zeatin; zinc
Abstract:
... Exacum bicolor, an endemic and endangered medicinal plant, belongs to the family Gentianaceae. A rapid protocol has been developed for efficient multiple shoot induction by testing nodal explants on Murashige and Skoog (MS) medium supplemented with various cytokinins. The cytokinins 6-benzyladenine (BA), 6-furfurylaminopurine (Kn), 2-isopentenyladenine (2-iP) and zeatin (Zn) were used individually ...
DOI:
10.1007/s11627-015-9726-5

2. A somatic embryogenesis–based system for the production of fluted pumpkin (Telfairia occidentalis Hook. f.) planting materials

Author:
Awosika, D. O.; Uchendu, E. E.; Balogun, M. O.; Adetimirin, V. O.
Source:
In vitro cellular & developmental biology 2015 v.51 no.6 pp. 612-618
ISSN:
1054-5476
Subject:
pumpkins; kinetin; cultivars; somatic embryos; germination; viability; plantlets; vigor; acclimation; cotyledons; Telfairia occidentalis; leaves; indole acetic acid; planting; in vitro studies; 2,4-D; roots; callus; shoots
Abstract:
... Fluted pumpkin (Telfairia occidentalis Hook. f.) is traditionally propagated by seeds, which have low viability after pod harvest, low percentage germination, and poor root development and often germinate during storage. The competition between use of seeds for consumption and propagation causes scarcity of propagules, necessitating development of more efficient propagation systems. Efficient prot ...
DOI:
10.1007/s11627-015-9728-3

3. Cryopreservation of in vitro-grown shoot tips of Cleome rosea Vahl (Cleomaceae) using the V cryo-plate technique Plant

Author:
da Silva Cordeiro, Lívia; Simões-Gurgel, Claudia; Albarello, Norma; Engelmann, Florent
Source:
In vitro cellular & developmental biology 2015 v.51 no.6 pp. 688-695
ISSN:
1054-5476
Subject:
Cleome; aluminum; benzyladenine; cryopreservation; growth regulators; in vitro studies; light intensity; vitrification
Abstract:
... This report highlights the first successful cryopreservation of in vitro shoot tips of Cleome rosea, achieved by the vitrification technique using aluminum cryo-plates (V cryo-plate). The effects on survival and recovery of C. rosea shoot tips of different plant vitrification solutions (PVS2 and PVS3), light intensity (standard and dim light), and 6-benzyladenine (BA) supplementation (0.10, 0.25, ...
DOI:
10.1007/s11627-015-9714-9

4. Eucalyptus dunnii Maiden plant regeneration via shoot organogenesis on a new basal medium based on the mineral composition of young stump shoots Plant

Author:
Oberschelp, Gustavo Pedro Javier; Gonçalves, Antônio Natal; Meneghetti, Eveline Calderan; Graner, Érika Mendes; de Almeida, Marcílio
Source:
In vitro cellular & developmental biology 2015 v.51 no.6 pp. 626-636
ISSN:
1054-5476
Subject:
Eucalyptus; acetic acid; benzyladenine; buds; callus; cotyledons; culture media; explants; genetic transformation; hypocotyls; in vitro studies; indole acetic acid; indole butyric acid; mineral content; naphthaleneacetic acid; organogenesis; propagation materials; reforestation; rooting; shoots; tissue culture
Abstract:
... Eucalyptus dunnii is an excellent alternative for afforestation and reforestation in frost-prone areas; however, it has limitations in the availability of propagation material. Given the great potential of tissue culture techniques for plant propagation and plant genetic transformation, the competence for in vitro organogenesis from E. dunnii cotyledons and hypocotyls was assessed. Several combina ...
DOI:
10.1007/s11627-015-9715-8

5. From leaf explants to rooted plantlets in a mist reactor Plant

Author:
Fei, Liwen; Weathers, Pamela
Source:
In vitro cellular & developmental biology 2015 v.51 no.6 pp. 669-681
ISSN:
1054-5476
Subject:
Artemisia annua; acclimation; air; artemisinin; biomass; carbon dioxide; carbon dioxide enrichment; explants; flavonoids; hyperhydricity; in vitro studies; leaves; light intensity; lignin; micropropagation; plantlets; relative humidity; root growth; rooting; roots; shoots; stems; stomatal movement; trichomes; water content
Abstract:
... The medicinally important plant Artemisia annua was used as an example to study the effect of mist reactor conditions on shooting, rooting, and in vitro acclimatization. During all three stages, plants grown in the mist reactor yielded more shoot biomass than corresponding gelled medium controls. During shoot development, shooting percentage and shoot multiplication increased with increased light ...
DOI:
10.1007/s11627-015-9727-4

6. Increased CaCl2, MgSO4, and KH2PO4 improve the growth of micropropagated red raspberries Plant

Author:
Poothong, Sukalya; Reed, Barbara M.
Source:
In vitro cellular & developmental biology 2015 v.51 no.6 pp. 648-658
ISSN:
1054-5476
Subject:
Rubus idaeus; calcium chloride; cultivars; culture media; genetic variation; in vitro studies; leaves; magnesium sulfate; micropropagation; mineral content; minerals; models; potassium dihydrogen phosphate; response surface methodology; shoots
Abstract:
... The genetic variation present in red raspberries makes it difficult to successfully apply a standard in vitro growth medium. An initial study modeling Murashige and Skoog (MS) medium minerals concluded that the mesos (CaCl₂, MgSO₄, and KH₂PO₄) components significantly affected red raspberry (Rubus idaeus L.) shoot quality and growth. To determine the effects of the individual mesos components, a t ...
DOI:
10.1007/s11627-015-9720-y

7. In vitro approaches for conservation of Asparagus racemosus Willd. Plant

Author:
Thakur, Shubha; Tiwari, K. L.; Jadhav, S. K.
Source:
In vitro cellular & developmental biology 2015 v.51 no.6 pp. 619-625
ISSN:
1054-5476
Subject:
Asparagus racemosus; branching; calcium alginate; encapsulation; ex situ conservation; explants; germplasm; habitats; in vitro studies; mannitol; medicinal plants; micropropagation; pharmaceutical industry; photoperiod; shoots; sorbitol; sucrose; temperature; India
Abstract:
... Asparagus racemosus is a commercially important medicinal plant, traditionally used for combating gynecological problems in India. The majority of plants used by the pharmaceutical industry come from wild sources, endangering the natural population of the species. The plants are being overharvested, so this species faces a real danger of becoming vulnerable in its natural habitat. Ex situ conserva ...
DOI:
10.1007/s11627-015-9706-9

8. In vitro propagation of the endangered species Berberis aristata DC. via leaf-derived callus Plant

Author:
Brijwal, Latika; Pandey, Aseesh; Tamta, Sushma
Source:
In vitro cellular & developmental biology 2015 v.51 no.6 pp. 637-647
ISSN:
1054-5476
Subject:
Berberis aristata; altitude; benzyladenine; callus; endangered species; gibberellic acid; greenhouse production; in vitro regeneration; in vitro studies; indole butyric acid; naphthaleneacetic acid; plantlets; rooting; roots; seed germination; seedlings; seeds; shoots; survival rate; thidiazuron; wet season; woody plants
Abstract:
... Berberis aristata, an endangered plant, is an important component in different medicinal formulations. The low seed germination rate coupled with fructification at high altitudes during the rainy season makes the task of seed collection difficult. In order to increase the yield of relevant plant material for medicinal and conservation purposes, an efficient in vitro regeneration protocol is import ...
DOI:
10.1007/s11627-015-9716-7

9. Palm (Arecaceae) somatic embryogenesis Plant

Author:
Ree, Joseph Francis; Guerra, Miguel Pedro
Source:
In vitro cellular & developmental biology 2015 v.51 no.6 pp. 589-602
ISSN:
1054-5476
Subject:
Elaeis guineensis; acclimation; explants; gene expression; genotype; histology; in vitro studies; inoculation methods; micropropagation; plantlets; somatic embryogenesis; somatic embryos; tissue culture
Abstract:
... Tissue culture comprises several techniques for the mass propagation of selected genotypes. In the case of palms, the most promising and efficient micropropagation technique is somatic embryogenesis. Ever since somatic embryogenesis was first induced in oil palm several decades ago, 19 palm species have been induced to develop somatic embryos from several types of explants. Optimization of somatic ...
DOI:
10.1007/s11627-015-9722-9

10. Phytostimulatory and hardening period-reducing effects of plant-associated bacteria on micropropagated Musa acuminata cv. Grand Naine Plant

Author:
Suada, E. P.; Jasim, B.; Jimtha, C. J.; Gayatri, G. P.; Radhakrishnan, E. K.; Remakanthan, A.
Source:
In vitro cellular & developmental biology 2015 v.51 no.6 pp. 682-687
ISSN:
1054-5476
Subject:
Bacillus (bacteria); Musa acuminata; Pseudomonas putida; acclimation; bacteria; cost effectiveness; in vitro studies; leaves; micropropagation; roots
Abstract:
... The effects of microorganisms on the health and growth of tissue-cultured plants is not well studied. In the current study, treatment of tissue culture-raised Musa acuminata cv. Grand Naine plants with culture supernatants of Pseudomonas putida, Pseudomonas flourescens, and Bacillus sp. had beneficial effects on acclimatization and hardening. At the hardening phase, there was an enhancement of var ...
DOI:
10.1007/s11627-015-9721-x

11. The extracts from Panax quinquefolium shoots derived from somatic embryos accumulate ginsenosides and have the antioxidant properties Plant

Author:
Kochan, Ewa; Szymańska, Grażyna; Grzegorczyk-Karolak, Izabela
Source:
In vitro cellular & developmental biology 2015 v.51 no.6 pp. 696-701
ISSN:
1054-5476
Subject:
Panax quinquefolius; aerial parts; antioxidant activity; callus; free radicals; gallic acid; ginsenosides; high performance liquid chromatography; in vitro studies; shoots; somatic embryos
Abstract:
... The present study was carried out to obtain somatic embryo-derived shoot cultures of Panax quinquefolium (American ginseng) and to examine the ginsenoside and total phenolic content (TPC) of these cultures. HPLC analysis revealed that the levels of six ginsenosides measured in shoot cultures totaled 9.76 mg g⁻¹ DW. TPC content was investigated using the Folin–Ciocalteu method and interpreted as ga ...
DOI:
10.1007/s11627-015-9730-9

12. Transgene integration complexity and expression stability following biolistic or Agrobacterium-mediated transformation of sugarcane Plant

Author:
Wu, Hao; Awan, Faisal Saeed; Vilarinho, Aloisio; Zeng, Qianchun; Kannan, Baskaran; Phipps, Tenisha; McCuiston, Jamie; Wang, Wenling; Caffall, Kerry; Altpeter, Fredy
Source:
In vitro cellular & developmental biology 2015 v.51 no.6 pp. 603-611
ISSN:
1054-5476
Subject:
Agrobacterium; Saccharum; Southern blotting; biofuels; biolistics; cultivars; enzyme-linked immunosorbent assay; feedstocks; fuel production; gene expression; genetically modified organisms; hybrids; in vitro studies; quantitative polymerase chain reaction; sugarcane; tissue culture; transfer DNA; transgenes; white sugar
Abstract:
... Sugarcane (Saccharum spp. hybrids) accounts for 80% of the table sugar produced worldwide and is also a prime feedstock for biofuel production. However, very few studies are available for directly comparing Agrobacterium tumefaciens-mediated transfer of T-DNA (AMT) and biolistic transfer of minimal expression cassettes (BLT MC) regarding transgene complexity and expression stability. In this study ...
DOI:
10.1007/s11627-015-9710-0

13. Caloric restriction mimetic 2-deoxyglucose maintains cytoarchitecture and reduces tau phosphorylation in primary culture of mouse hippocampal pyramidal neurons

Author:
Bele, M. S.; Gajare, K. A.; Deshmukh, A. A.
Source:
In vitro cellular & developmental biology 2015 v.51 no.6 pp. 546-555
ISSN:
1071-2690
Subject:
2-deoxyglucose; Western blotting; brain; fluorescent antibody technique; glycogen (starch) synthase; low calorie diet; mice; microtubules; neurites; neurodegenerative diseases; phosphorylation; proteins; tau-protein kinase; vacuoles
Abstract:
... Typical form of neurons is crucially important for their functions. This is maintained by microtubules and associated proteins like tau. Hyperphosphorylation of tau is a major concern in neurodegenerative diseases. Glycogen synthase kinase3β (GSK3β) and cyclin-dependent protein kinase 5 (Cdk5) are the enzymes that govern tau phosphorylation. Currently, efforts are being made to target GSK3β and Cd ...
DOI:
10.1007/s11626-015-9867-1

14. Derivation of embryonic stem cells from Kunming mice IVF blastocyst in feeder- and serum-free condition

Author:
Liu, Xiaokun; Wei, Qiang; Zhang, Junhong; Yang, Wanli; Zhao, Xiaoe; Ma, Baohua
Source:
In vitro cellular & developmental biology 2015 v.51 no.6 pp. 541-545
ISSN:
1071-2690
Subject:
alkaline phosphatase; blood serum; embryonic stem cells; gene expression; glycogen synthase kinases; hatching; in vitro fertilization; mice; trophoblast; China
Abstract:
... Kunming mice are widely used in China; however, it is difficult to isolate embryonic stem cells (ESCs) in conventional derivation condition containing feeder cells and serum. 6-Bromoindirubin-3′-oxime (BIO), a glycogen synthase kinase 3 (GSK3) inhibitor, could facilitate the maintenance of pluripotency of ESCs. Therefore, BIO could be considered as a candidate to replace feeder cells and serum. On ...
DOI:
10.1007/s11626-014-9863-x

15. Development of a sensitive in vitro assay to quantify the biological activity of pro-inflammatory phorbol esters in Jatropha oil

Author:
Pelletier, Guillaume; Padhi, Bhaja K.; Hawari, Jalal; Sunahara, Geoffrey I.; Poon, Raymond
Source:
In vitro cellular & developmental biology 2015 v.51 no.6 pp. 644-650
ISSN:
1071-2690
Subject:
Jatropha; bioassays; biodiesel; direct contact; dogs; dose response; esters; feedstocks; gene expression regulation; genes; in vitro studies; kidneys; mixtures; oils; prostaglandin synthase; protein kinase C; quantitative analysis; risk assessment; toxicity; transcription (genetics)
Abstract:
... New health safety concerns may arise from the increasing production and use of Jatropha oil, a biodiesel feedstock that also contains toxic, pro-inflammatory, and co-carcinogenic phorbol esters. Based on the exceptional sensitivity of Madin-Darby canine kidney (MDCK) cells to the model phorbol ester 12-O-tetradecanoylphorbol-13-acetate (TPA), a robust bioassay was developed to quantify the biologi ...
DOI:
10.1007/s11626-014-9861-z

16. Differential characteristics of CD133+ and CD133− Jurkat cells

Author:
Anbarlou, Azadeh; Atashi, Amir; Soleimani, Masoud; AkhavanRahnama, Mahshid; Bohloli, Mahbobeh; Mossahebi-Mohammadi, Majid
Source:
In vitro cellular & developmental biology 2015 v.51 no.6 pp. 556-561
ISSN:
1071-2690
Subject:
T-lymphocytes; apoptosis; drug resistance; drug therapy; genes; hematologic diseases; lymphocytic leukemia; patients; resistance mechanisms; stem cells
Abstract:
... T cell acute lymphoblastic leukemia (T-ALL) is a hematological disease including malignancy of T cell precursors. There are some T-ALL patients that are drug-resistant. A major cause of treatment failure in cancers can be associated with the existence of cancer stem cells. The identification of these cell populations helps us to clarify resistance mechanisms and rely on special markers for recogni ...
DOI:
10.1007/s11626-015-9869-z

17. Differentiation of human skin-derived precursor cells into functional islet-like insulin-producing cell clusters

Author:
Mehrabi, Maryam; Mansouri, Kamran; Hosseinkhani, Saman; Yarani, Reza; Yari, Kheirollah; Bakhtiari, Mitra; Mostafaie, Ali
Source:
In vitro cellular & developmental biology 2015 v.51 no.6 pp. 595-603
ISSN:
1071-2690
Subject:
c-peptide; diabetes; dose response; enzyme-linked immunosorbent assay; gene expression; genes; glucagon; glucose; humans; reverse transcriptase polymerase chain reaction; secretion; somatostatin
Abstract:
... Advances in cell-replacement strategies for diabetes have focused on renewable sources of glucose-responsive, insulin-producing cells (IPCs). One of the most proper alternatives is multipotent skin-derived precursors cells (SKPs), which can be differentiated into IPCs. In this study, we reported the isolation and expansion of human skin-derived precursors (hSKPs) followed by their differentiation ...
DOI:
10.1007/s11626-015-9866-2

18. Establishment and characterization of a novel cell line from midgut tissue of Helicoverpa armigera (Lepidoptera: Noctuidae)

Author:
Li, Jianghuai; He, Fei; Yang, Yongbo; Xiao, Yutao; Peng, Rong; Yao, Hanchao; Li, Xianchun; Peng, Jianxin; Hong, Huazhu; Liu, Kaiyu
Source:
In vitro cellular & developmental biology 2015 v.51 no.6 pp. 562-571
ISSN:
1071-2690
Subject:
Autographa californica multiple nucleopolyhedrovirus; Bacillus thuringiensis; DNA; Helicoverpa armigera; apoptosis; cell culture; chromosome number; chromosomes; culture media; fetal bovine serum; insects; instars; larvae; midgut; pathogens
Abstract:
... The midgut of lepidopteran larvae serves as a target for many pathogens such as Bacillus thuringiensis (Bt). Cell lines originating from midgut tissues will be very helpful tools in many research fields. However, to date, no Bt-susceptible midgut-derived cell lines are available. Here, we reported that a novel cell line, designated as HNU-Ha-MG1, was established from midgut tissue of the fourth in ...
DOI:
10.1007/s11626-015-9870-6

19. In vitro antioxidant and hepatoprotective potential of Azolla microphylla phytochemically synthesized gold nanoparticles on acetaminophen – induced hepatocyte damage in Cyprinus carpio L.

Author:
Kunjiappan, Selvaraj; Bhattacharjee, Chiranjib; Chowdhury, Ranjana
Source:
In vitro cellular & developmental biology 2015 v.51 no.6 pp. 630-643
ISSN:
1071-2690
Subject:
Azolla microphylla; Cyprinus carpio; acetaminophen; alanine transaminase; antioxidant activity; antioxidants; catalase; culture media; fish; glutamic acid; glutathione peroxidase; hepatocytes; lactate dehydrogenase; malondialdehyde; methanol; nanogold; superoxide dismutase; viability
Abstract:
... The present study aims to evaluate the hepatoprotective and antioxidant effects of gold nanoparticles (GNaP) biosynthesized through the mediation of Azolla microphylla and A. microphylla extract on acetaminophen-induced hepatocyte damage in common carp fish (Cyprinus carpio L.). The gold nanoparticles (100, 150, 200 μg/ml) and A. microphylla extract powder (100, 200, 400 μg/ml) were added to the p ...
DOI:
10.1007/s11626-014-9841-3

20. Media formulation influences chemical effects on neuronal growth and morphology

Author:
Harrill, Joshua A.; Robinette, Brian L.; Freudenrich, Theresa M.; Mundy, William R.
Source:
In vitro cellular & developmental biology 2015 v.51 no.6 pp. 612-629
ISSN:
1071-2690
Subject:
blood serum; cell death; culture media; endpoints; image analysis; in vivo studies; neurons; neurotoxicity; neurotoxins; rats; screening; synaptogenesis
Abstract:
... Screening for developmental neurotoxicity using in vitro, cell-based systems has been proposed as an efficient alternative to performing in vivo studies. One tool currently used for developmental neurotoxicity screening is automated high-content imaging of neuronal morphology. While high-content imaging (HCI) has been demonstrated to be useful in detection of potential developmental neurotoxicants ...
DOI:
10.1007/s11626-015-9873-3