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- Author:
- CHANDLER, STEPHEN F.; LU, CHIN-YI
- Source:
- In vitro cellular & developmental biology 2005 v.41 no.5 pp. 591-601
- ISSN:
- 1054-5476
- Subject:
- biotechnology; genes; genetic engineering; ornamental horticulture; ornamental plants; transgenic plants
- Abstract:
- ... Genetic engineering techniques have so far had limited impact in the field of ornamental horticulture. As outlined in this review, transformation systems and potential genes of interest are available. As the development of new, novel varieties is an important driving force in the industry, there are, therefore, good prospects for the development of genetically modified ornamental varieties. The fe ...
- DOI:
- 10.1079/IVP2005681
-
http://dx.doi.org/10.1079%2FIVP2005681
- Author:
- SUNANDAKUMARI, C.; ZHANG, C.-L.; MARTIN, K. P.; SLATER, A.; MADHUSOODANAN, P. V.
- Source:
- In vitro cellular & developmental biology 2005 v.41 no.5 pp. 695-699
- ISSN:
- 1054-5476
- Subject:
- 2,4-D; Agrobacterium radiobacter; Euphorbia; callus; culture media; explants; gibberellic acid; indole butyric acid; kanamycin; kinetin; medicinal plants; naphthaleneacetic acid; organogenesis; plantlets; roots; shoots; somatic embryogenesis; somatic embryos; survival rate; transgenes; transgenic plants
- Abstract:
- ... The types of auxin in Murashige and Skoog (MS) medium containing N⁶-benzyladenine (BA) determined indirect morphogenesis, i.e. development to bipolar somatic embryos or monopolar shoots in Euphorbia nivulia Buch.-Ham. Indirect in vitro morphogenesis depended on growth regulators, explant excision period, and light. Calli induced from explants collected in March––April were superior in the inductio ...
- DOI:
- 10.1079/IVP2005651
-
http://dx.doi.org/10.1079%2FIVP2005651
- Author:
- YEUNG, EDWARD C.; BELMONTE, MARK F.; TU, LUU T. T.; STASOLLA, CLAUDIO
- Source:
- In vitro cellular & developmental biology 2005 v.41 no.5 pp. 584-590
- ISSN:
- 1054-5476
- Subject:
- cell proliferation; culture media; cultured cells; glutathione; homeostasis; in vitro studies; morphogenesis; plant development
- Abstract:
- ... It is well known that glutathione is a ubiquitous and multifunctional compound which is important in maintaining cellular redox homeostasis. The balance between the reduced (GSH) and oxidized (GSSG) forms of this tripeptide plays a fundamental role in basic physiological and metabolic processes in plants. Recently, a remarkable amount of evidence has suggested that the role of glutathione within t ...
- DOI:
- 10.1079/IVP2005683
-
http://dx.doi.org/10.1079%2FIVP2005683
- Author:
- MERKLE, SCOTT A.; NAIRN, C. JOSEPH
- Source:
- In vitro cellular & developmental biology 2005 v.41 no.5 pp. 602-619
- ISSN:
- 1054-5476
- Subject:
- Eucalyptus; Populus; biotechnology; conifers; disease resistance; expressed sequence tags; forest trees; forestry; gene transfer; genetic markers; genetically modified organisms; genomics; hardwood; hardwood forests; insects; phytoremediation; sequence analysis; somatic embryogenesis; wood quality
- Abstract:
- ... Due to the commercial importance of some conifer species, advances in conifer biotechnology often appear to overshadow equally significant advances in the biotechnology of angiosperm forest trees. However, progress with some hardwood forest trees has been just as promising as that made with conifers, and in some areas, has surpassed conifer biotechnology, particularly in the past few years. Until ...
- DOI:
- 10.1079/IVP2005687
-
http://dx.doi.org/10.1079%2FIVP2005687
- Author:
- HU, ZHONG; LI, WEI; GUO, GUANG-QIN
- Source:
- In vitro cellular & developmental biology 2005 v.41 no.5 pp. 662-665
- ISSN:
- 1054-5476
- Subject:
- Incarvillea; adventitious shoots; benzyladenine; callus; cotyledons; explants; indole acetic acid; plantlets; roots; soil
- Abstract:
- ... A simple and effective procedure has been developed for plantlet regeneration from cotyledon-derived callus of the medicinally important herb and ornamental species, Incarvillea sinensis. An average of 18.4 adventitious shoots per explant were obtained from 100%% cotyledon explants cultured on half-strength Murashige and Skoog (MS) medium containing 1.0 mg l⁻⁻¹ 6-benzylaminopurine for 3 wk, follow ...
- DOI:
- 10.1079/IVP2005682
-
http://dx.doi.org/10.1079%2FIVP2005682
- Author:
- ZHANG, T.; CAO, Z. Y.; WANG, X. Y.
- Source:
- In vitro cellular & developmental biology 2005 v.41 no.5 pp. 655-657
- ISSN:
- 1054-5476
- Subject:
- 2,4-D; Eruca vesicaria subsp. sativa; callus; cotyledons; explants; hypocotyls; indole butyric acid; plantlets; polyethylene glycol; somatic embryogenesis; somatic embryos
- Abstract:
- ... A protocol was developed for high frequency somatic embryogenesis and plant regeneration from cotyledon and hypocotyl explants of Eruca sativa. Explants grown on Murashige and Skoog (MS) medium supplemented with 4.52 μμM 2,4-D formed embryogenic callus after 4 wk of culture. Secondary somatic embryos were also produced from primary somatic embryos on MS medium containing 0.56 μμM 2,4-D. Somatic em ...
- DOI:
- 10.1079/IVP2005653
-
http://dx.doi.org/10.1079%2FIVP2005653
- Author:
- SUDHA, C. G.; Krishnan, P. N.; Pushpangadan, P.; Seeni, S.
- Source:
- In vitro cellular & developmental biology 2005 v.41 no.5 pp. 648-654
- ISSN:
- 1054-5476
- Subject:
- Apocynaceae; benzyladenine; branching; explants; forests; in vitro regeneration; isopentenyladenine; micropropagation; naphthaleneacetic acid; rooting; roots; seedlings; shoots
- Abstract:
- ... This study reports a protocol for successful micropropagation of Decalepis arayalpathra (Joseph and Chandras) Venter. (Janakia arayalpathra Joseph and Chandrasekhran; Periplocaceae), a critically endangered and endemic ethnomedicinal plant in the southern forests of the Western Ghats which is overexploited for its tuberous medicinal roots by the local Kani tribes. Natural regeneration is rare and ...
- DOI:
- 10.1079/IVP2005652
-
http://dx.doi.org/10.1079%2FIVP2005652
- Author:
- SHIAU, YIH-JUH; NALAWADE, SATISH MANOHAR; HSIA, CHI-NI; MULABAGAL, VANISREE; TSAY, HSIN-SHENG
- Source:
- In vitro cellular & developmental biology 2005 v.41 no.5 pp. 666-670
- ISSN:
- 1054-5476
- Subject:
- Dendrobium; Oriental traditional medicine; activated carbon; benzyladenine; buds; culture media; explants; medicinal plants; naphthaleneacetic acid; plantlets; pollination; potatoes; seedlings; seeds; shoots; soil; sucrose
- Abstract:
- ... Dendrobium candidum Wall. Ex Lindl. is an important species used in the formulation of Shih-hu, a Chinese traditional medicine. An efficient protocol for in vitro propagation of D. candidum using the axenic nodal segments of the shoots, originated from the in vitro germinated seedlings, was developed. The seeds from 120-d-old capsules after pollination were first germinated on half-strength Murash ...
- DOI:
- 10.1079/IVP2005685
-
http://dx.doi.org/10.1079%2FIVP2005685
- Author:
- DHAKA, NEETI; KOTHARI, S. L.
- Source:
- In vitro cellular & developmental biology 2005 v.41 no.5 pp. 658-661
- ISSN:
- 1054-5476
- Subject:
- Eclipta prostrata; buds; explants; gibberellic acid; indole butyric acid; medicinal plants; micropropagation; plantlets; rooting; shoots
- Abstract:
- ... An efficient and reproducible protocol for mass propagation of Eclipta alba (L.) Hassk, an important medicinal plant, was standardized by culturing shoot tips and nodal segments taken from in vitro raised plants. Maximum shoot proliferation occurred when the explants were cultured on Murashige and Skoog (MS) medium supplemented with 1 mg l⁻⁻¹ benzylaminopurine (BAP). The shoot buds formed were fur ...
- DOI:
- 10.1079/IVP2005684
-
http://dx.doi.org/10.1079%2FIVP2005684
- Author:
- JAYAPAUL, K.; KISHOR, P. B. KAVI; REDDY, K. JANARDHAN
- Source:
- In vitro cellular & developmental biology 2005 v.41 no.5 pp. 682-685
- ISSN:
- 1054-5476
- Subject:
- 2,4-D; Justicia adhatoda; benzyladenine; bioactive properties; callus; callus culture; explants; high performance liquid chromatography; kinetin; mass spectrometry; naphthaleneacetic acid; nuclear magnetic resonance spectroscopy; petioles; thin layer chromatography
- Abstract:
- ... Callus cultures of Adhatoda zeylanica Medicus were established from leaf and petiole explants. Accumulation of a bioactive pyrroloquinazoline alkaloid, vasicine, in callus cultures was detected and confirmed by thin layer chromatography, electron-ionization mass spectra, ¹³C NMR and high-pressure liquid chromatography analysis. The mass of vasicine obtained from leaf-derived callus cultures was fo ...
- DOI:
- 10.1079/IVP2005679
-
http://dx.doi.org/10.1079%2FIVP2005679
- Author:
- MOÏÏSE, JAIMIE A.; HAN, SHUYOU; GUDYNAITĘĘ-SAVITCH, LORETA; JOHNSON, DOUGLAS A.; MIKI, BRIAN L. A.
- Source:
- In vitro cellular & developmental biology 2005 v.41 no.5 pp. 620-644
- ISSN:
- 1054-5476
- Subject:
- seed dormancy; testa; germination; Fabaceae; biotechnology; genes; molecular genetics; Brassicaceae; seed quality; embryogenesis; molecular biology; tissues
- Abstract:
- ... Although seeds have been the subject of extensive studies for many years, their seed coats are just beginning to be examined from the perspective of molecular genetics and control of development. The seed coat plays a vital role in the life cycle of plants by controlling the development of the embryo and determining seed dormancy and germination. Within the seed coat are a number of unique tissues ...
- DOI:
- 10.1079/IVP2005686
-
http://dx.doi.org/10.1079%2FIVP2005686
- Author:
- SHARMA, P.; KOCHE, VIJAYA; QURAISHI, AFAQUE; MISHRA, S. K.
- Source:
- In vitro cellular & developmental biology 2005 v.41 no.5 pp. 645-647
- ISSN:
- 1054-5476
- Subject:
- 2,4-D; Buchanania; abscisic acid; benzyladenine; dry forests; fruit trees; genetic engineering; germination; naphthaleneacetic acid; plantlets; somatic embryogenesis; somatic embryos; India
- Abstract:
- ... We report a protocol for somatic embryogenesis and plantlet regeneration of Buchanania lanzan Spreng (Family –– Anacardiaceae), which is a tropical fruit tree widely distributed in the dry forests of India. Calluses were initiated from immature zygotic embryos cultured on Murashige and Skoog (MS) medium supplemented with various combinations of 2,4-dichlorophenoxyacetic acid (2,4-D), 6-benzyladeni ...
- DOI:
- 10.1079/IVP2005680
-
http://dx.doi.org/10.1079%2FIVP2005680
- Author:
- MIKUłłA, ANNA; TYKARSKA, TERESA; KURAŚŚ, MIECZYSłłAW; RYBCZYŃŃSKI, JAN J.
- Source:
- In vitro cellular & developmental biology 2005 v.41 no.5 pp. 686-694
- ISSN:
- 1054-5476
- Subject:
- Gentiana; callus; callus formation; cortex; cotyledons; cytokinesis; explants; hypocotyls; organelles; seedlings; somatic embryogenesis; somatic embryos; tissue culture; ultrastructure
- Abstract:
- ... Structure and ultrastructure changes that occurred during tissue culture of upper explants of hypocotyl (adjacent to cotyledons) of 10-d-old seedlings of Gentiana cruciata were studied. The explants were cultured on Murashige and Skoog induction medium supplemented with 1.0 mg l⁻⁻¹ dicamba++0.1 mg l⁻⁻¹ naphthaleneacetic acid++2.0 mg l⁻⁻¹ benzyladenine++80.0 mg l⁻⁻¹ adenine sulfate. The initial res ...
- DOI:
- 10.1079/IVP2005678
-
http://dx.doi.org/10.1079%2FIVP2005678
- Author:
- DEBNATH, SAMIR C.
- Source:
- In vitro cellular & developmental biology 2005 v.41 no.5 pp. 671-676
- ISSN:
- 1054-5476
- Subject:
- Fragaria; adventitious shoots; buds; callus; callus formation; calyx; culture media; cytokinins; petioles; plantlets; strawberries; thidiazuron; urea; zeatin
- Abstract:
- ... An efficient system to regenerate shoots on excised sepals (calyx) of greenhouse-grown ‘‘Bounty’’ strawberry (Fragaria ×× ananassa Duch.) was developed in vitro. Sepal cultures produced multiple buds and shoots without an intermediary callus phase on 2––4 μμM 1-phenyl-3-(1,2,3-thiadiazol-5-yl) urea (thidiazuron, TDZ)-containing shoot induction medium within 4––5 wk of culture initiation. Young exp ...
- DOI:
- 10.1079/IVP2005688
-
http://dx.doi.org/10.1079%2FIVP2005688
- Author:
- JIANG, BO; YANG, YING-GEN; GUO, YI-MING; GUO, ZHONG-CHEN; CHEN, YONG-ZHE
- Source:
- In vitro cellular & developmental biology 2005 v.41 no.5 pp. 677-681
- ISSN:
- 1054-5476
- Subject:
- Arnebia euchroma; benzyladenine; cotyledons; cytokinins; explants; genetic improvement; hypocotyls; indole butyric acid; kinetin; medicinal plants; micropropagation; organogenesis; roots; shoots; thidiazuron
- Abstract:
- ... An efficient in vitro propagation system was developed for Arnebia euchroma, an important Chinese traditional medicinal plant. The present study utilized thidiazuron (TDZ) for the induction of shoot organogenesis on cotyledon and hypocotyl explants. The maximal number of shoots was obtained on the modified Linsmaier and Skoog (LS) medium supplemented with 1.0 mg l⁻⁻¹ (4.5 μμM) TDZ for 12 d on coty ...
- DOI:
- 10.1079/IVP2005650
-
http://dx.doi.org/10.1079%2FIVP2005650
- Author:
- KAMITA, SHIZUO G.; DO, ZUNG N.; SAMRA, AMAN I.; HAGLER, JAMES R.; HAMMOCK, BRUCE D.
- Source:
- In vitro cellular & developmental biology 2005 v.41 no.5 pp. 149
- ISSN:
- 1543-706X
- Subject:
- Homalodisca vitripennis; insect pests; cell lines; cell culture; embryo (animal); culture media
- Abstract:
- ... Four continuous cell lines were established from the embryos of the glassy-winged sharpshooter, Homalodisca coagulata (Say), an economically important insect vector of bacterial pathogens of grape, almond, citrus, oleander, and other agricultural and ornamental plantings. The cell lines were designated GWSS-Z10, GWSS-Z15, GWSS-G3, and GWSS-LH. The GWSS-Z10, GWSS-Z15, and GWSS-G3 lines were culture ...
- Handle:
- 10113/39830
- DOI:
- 10.1290/0501002.1
-
http://dx.doi.org/10.1290/0501002.1