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You searched for: Journal In vitro cellular & developmental biology Remove constraint Journal: In vitro cellular & developmental biology Publication Year 2004 Remove constraint Publication Year: 2004 Source 2004 v.40 no.4 Remove constraint Source: 2004 v.40 no.4
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1. Adventitious shoot bud formation and plant regeneration from in vitro-cultured stem segments of reed (phragmites communis trin) Plant

Author:
GUO, YI-MING; YANG, YING-GEN; GUO, YI; GUO, ZHONG-CHEN
Source:
In vitro cellular & developmental biology 2004 v.40 no.4 pp. 412-415
ISSN:
1054-5476
Subject:
2,4-D; Phragmites australis; buds; explants; gene transfer; indole butyric acid; micropropagation; naphthaleneacetic acid; salt tolerance; shoots; soil
Abstract:
... A protocol for large-scale propagation of Phragmites communis Trin. by adventitious bud formation and plant regeneration was established. Adventitious buds were induced through either the indirect pathway or the direct pathway from stem explants of Phragmites communis. In the indirect pathway, it was essential to decrease the level of 2,4-dichlorophenoxyacetic acid from 9.1 to 0.5 μμM to induce ad ...
DOI:
10.1079/IVP2004553

2. Cryopreservation of african violet (saintpaulia ionantha wendl.) shoot tips Plant

Author:
MOGES, ASMARA D.; SHIBLI, RIDA A.; KARAM, NABILA S.
Source:
In vitro cellular & developmental biology 2004 v.40 no.4 pp. 389-395
ISSN:
1054-5476
Subject:
Saintpaulia ionantha; air drying; cryopreservation; dimethyl sulfoxide; direct contact; encapsulation; freezing; glycerol; regrowth; shoots; silica gel; sucrose; viability; vitrification
Abstract:
... Cryopreservation of African violet via encapsulation––dehydration, vitrification, and encapsulation––vitrification of shoot tips was evaluated. Encapsulation––dehydration, pretreatment of shoot tips with 0.3 M sucrose for 2 d followed by air dehydration for 2 and 4 h resulted in complete survival and 75%% regrowth, respectively. Dehydration of encapsulated shoot tips with silica gel for 1 h result ...
DOI:
10.1079/IVP2004536

3. Cucurbit biotechnology –– the importance of virus resistance Plant

Author:
GABA, VICTOR; ZELCER, AARON; GAL-ON, AMIT
Source:
In vitro cellular & developmental biology 2004 v.40 no.4 pp. 346-358
ISSN:
1054-5476
Subject:
Cucurbitaceae; biotechnology; crops; cucumbers; disease resistance; field experimentation; pumpkins; squashes; transgenic plants; viruses; watermelons; United States
Abstract:
... The cucurbit family includes a number of valuable crop species (melon, cucumber, squash/pumpkin, watermelon). Much of this review is concerned with transgenic resistance to viruses, shown to be the major application of biotechnology in the cucurbit family. Progress made with the production of transgenic cucurbit crops is discussed. Published data on field tests of transgenic cucurbits are reviewed ...
DOI:
10.1079/IVP2004554

4. Different sealing materials for petri dishes strongly affect shoot regeneration and development from leaf explants of quince ‘‘ba 29’’ Plant

Author:
MARINO, GRAZIA; BERARDI, GABRIELLA
Source:
In vitro cellular & developmental biology 2004 v.40 no.4 pp. 384-388
ISSN:
1054-5476
Subject:
Cydonia oblonga; agar; benzyladenine; carbon dioxide; cefotaxime; ethylene; explants; exposure duration; leaves; naphthaleneacetic acid; photoperiod; photosynthetically active radiation; poly(vinyl chloride); quinces; shoots; thidiazuron
Abstract:
... The effect of different sealing materials \[i.e., polyvinyl chloride (PVC) transparent film, and Parafilm (PARA)\] for Petri dishes was investigated on shoot regeneration from quince (Cydonia oblonga L.) ‘‘BA 29’’ leaf explants. Leaves were excised from proliferating shoot cultures, transversally scored, and placed with the abaxial side down in 60-mm Petri dishes containing 10 ml of Murashige and ...
DOI:
10.1079/IVP2004550

5. Efficient agrobacterium tumefaciens-mediated transformation of sweet potato (ipomoea batatas (l.) lam.) from stem explants using a two-step kanamycin––hygromycin selection method Plant

Author:
SONG, GUO-QING; HONDA, HIDEO; YAMAGUCHI, KEN-ICHI
Source:
In vitro cellular & developmental biology 2004 v.40 no.4 pp. 359-365
ISSN:
1054-5476
Subject:
Agrobacterium radiobacter; Ipomoea batatas; Southern blotting; abscisic acid; cefotaxime; coculture; cultivars; explants; genetically modified organisms; gibberellic acid; kanamycin; petioles; photoperiod; polymerase chain reaction; reporter genes; shoots; sweet potatoes; transfer DNA
Abstract:
... To achieve reliable stable transformation of sweet potato, we first developed efficient shoot regeneration for stem explants, leaf disks, and petioles of sweet potato (Ipomoea batatas (L.) Lam.) cultivar Beniazuma. The shoot regeneration protocol enabled reproducible stable transformation mediated by Agrobacterium tumefaciens strain EHA105. The binary vector pIG121Hm contains the npt II (pnos) gen ...
DOI:
10.1079/IVP2004539

6. Enhancing the productivity of hybrid yellow-poplar and hybrid sweetgum embryogenic cultures Plant

Author:
DAI, JIANLIANG; VENDRAME, WAGNER A.; MERKLE, SCOTT A.
Source:
In vitro cellular & developmental biology 2004 v.40 no.4 pp. 376-383
ISSN:
1054-5476
Subject:
2,4-D; Liquidambar; Liriodendron tulipifera; abscisic acid; arginine; asparagine; benzyladenine; casein; embryogenesis; fractionation; genotype; greenhouses; hybrids; hydrolysates; pH; seeds; somatic embryos
Abstract:
... High-frequency embryogenesis systems were established for hybrid yellow-poplar (Liriodendron tulipifera××L. chinense) and hybrid sweetgum (Liquidambar styraciflua××L. formosana) by modifying a medium originally developed for embryogenic yellow-poplar cultures. Embryogenic cultures of both hybrids, consisting of proembryogenic masses (PEMs), were initiated from immature hybrid seeds on an induction ...
DOI:
10.1079/IVP2004538

7. EXPRESSION OF THE rolB GENE ENHANCES ADVENTITIOUS ROOT FORMATION IN HARDWOOD CUTTINGS OF ASPEN Plant

Author:
DAI, WENHAO; CHENG, ZONG-MING; SARGENT, WAYNE A.
Source:
In vitro cellular & developmental biology 2004 v.40 no.4 pp. 366-370
ISSN:
1054-5476
Subject:
Agrobacterium radiobacter; Populus canescens; Populus grandidentata; Southern blotting; adventitious roots; auxins; genes; genetic transformation; hardwood; heat; heat shock response; hybrids; polymerase chain reaction; rooting; transgenic plants; woody plants
Abstract:
... An elite aspen hybrid (Populus××canescens××P. grandidentata) was transformed with Agrobacterium tumefaciens strain EHA105 that harbored a binary vector (pBI121) carrying the nptII gene under the nos promoter and tandem rolB––uidA (GUS) genes with the CaMV 35S or heat shock promoter. Among 32 independent kanamycin-resistant plants, 25 plants were confirmed by polymerase chain reaction and Southern ...
DOI:
10.1079/IVP2004556

8. Factors affecting somatic embryogenesis and plant regeneration from a range of recalcitrant genotypes of chinese cottons (gossypium hirsutum l) Plant

Author:
WU, JIAHE; ZHANG, XIANLONG; NIE, YICHUN; JIN, SHUANGXIA; LIANG, SHAOGUANG
Source:
In vitro cellular & developmental biology 2004 v.40 no.4 pp. 371-375
ISSN:
1054-5476
Subject:
Gossypium hirsutum; biotechnology; breeding; cotton; cultivars; genotype; indole butyric acid; kinetin; plantlets; potassium nitrate; somatic embryogenesis; somatic embryos; tissue culture
Abstract:
... A new protocol has been developed for the highly efficient somatic embryogenesis and plant regeneration of 10 recalcitrant Chinese cotton cultivars. Calluses and embryogenic calluses were induced on MSB1 medium containing the optimal combination of indolebutyric acid (IBA; 2.46 μμM) and kinetin (KT; 2.32 μμM). Up to 86.7%% of embryogenic calluses differentiated into globular somatic embryos 2 mo. ...
DOI:
10.1079/IVP2004535

9. Improvement of jojoba shoot multiplication in vitro by ventilation Plant

Author:
Mills, David; Yanqing, Zhou; Benzioni, Aliza
Source:
In vitro cellular & developmental biology 2004 v.40 no.4 pp. 396-402
ISSN:
1054-5476
Subject:
Simmondsia chinensis; biomass; clones; developmental stages; exposure duration; genotype; hyperhydricity; jojoba; lids; micropropagation; shoots; stomatal movement
Abstract:
... The effect of ventilation during the multiplication stage on the development of propagules from different clones of jojoba \[Simmondsia chinensis (Link) Schneider\] was investigated. Variation in the response to ventilation was due to genotype, the extent of ventilation, and to the period of exposure (transfer number). With intermediate ventilation treatments, propagules elongated to a greater ext ...
DOI:
10.1079/IVP2004537

10. In vitro morphogenesis in plants –– recent advances Plant

Author:
PHILLIPS, GREGORY C.
Source:
In vitro cellular & developmental biology 2004 v.40 no.4 pp. 342-345
ISSN:
1054-5476
Subject:
biochemistry; breeding; genes; genomics; morphogenesis; plant tissues; transgenic plants
Abstract:
... The capacity of cultured plant tissues and cells to undergo morphogenesis, resulting in the formation of discrete organs or whole plants, has provided opportunities for numerous applications of in vitro plant biology in studies of basic botany, biochemistry, propagation, breeding, and development of transgenic crops. While the fundamental techniques to achieve in vitro plant morphogenesis have bee ...
DOI:
10.1079/IVP2004555

11. In vitro propagation and cryostorage of syzygium francissi (myrtaceae) by the encapsulation––dehydration method Plant

Author:
AWAD SHATNAWI, M.; JOHNSON, KRYSTYNA A.; TORPY, FRASER R.
Source:
In vitro cellular & developmental biology 2004 v.40 no.4 pp. 403-407
ISSN:
1054-5476
Subject:
Syzygium; benzyladenine; cryopreservation; encapsulation; germplasm; greenhouses; indole acetic acid; indole butyric acid; naphthaleneacetic acid; perlite; plantlets; roots; shoots; sucrose; survival rate; vermiculite; water content
Abstract:
... An efficient procedure for the in vitro propagation and cryogenic conservation of Syzygium francissi was developed. The maximum number of shoots per explant was obtained on a Murashige and Skoog (MS) medium supplemented with 4.5 μμM benzyladenine and 0.5 μμM indole-3-butyric acid (IBA). The in vitro-propagated shoots produced roots when transferred to MS medium containing IBA, indole-3-acetic acid ...
DOI:
10.1079/IVP2004551

12. Use of sugarcane bagasse as an alternative low-cost support material during the rooting stage of apple micropropagation Plant

Author:
MOHAN, R.; SOCCOL, C. R.; QUOIRIN, M.; PANDEY, A.
Source:
In vitro cellular & developmental biology 2004 v.40 no.4 pp. 408-411
ISSN:
1054-5476
Subject:
Malus prunifolia; agar; apples; cost effectiveness; indole butyric acid; micropropagation; particle size; rooting; roots; rootstocks; salts; shoots; sucrose; sugarcane bagasse; vitamins
Abstract:
... Studies were carried out to evaluate sugarcane bagasse as an alternative to agar for micropropagation of apple clones to reduce the cost of micropropagation and improve the quality of the propagules. Significant improvement in the in vitro rooting process, coupled with cost reduction, were obtained by the use of sugarcane bagasse as a substitute for the traditionally used agar-gelled medium. The t ...
DOI:
10.1079/IVP2004552