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- Bajwa, Paramjit K.; Harrington, Sean; Dashtban, Mehdi; Lee, Hung
- Industrial biotechnology 2016 v.12 no.2 pp. 98-104
- Betula; Fagus; Pichia pastoris; Scheffersomyces stipitis; acids; affinity chromatography; biotechnology; endo-1,4-beta-xylanase; enzyme activity; genes; hydrogels; hydrolysis; molecular weight; oats; pH; polyacrylamide gel electrophoresis; sodium dodecyl sulfate; solubility; synergism; temperature; xylan; xylooligosaccharides
- ... The gene encoding glycosyl hydrolase family 115 α-glucuronidase from Scheffersomyces stipitis (ssagu115) was cloned and expressed in Pichia pastoris strain X33. The recombinant enzyme was purified to homogeneity by Ni-chelation affinity chromatography. The apparent molecular weight of the secreted recombinant enzyme was about 150 kDa as determined by sodium dodecyl sulfate polyacrylamide gel elect ...