PubAg

Main content area

You searched for: Subject "parasites" Remove constraint Subject: "parasites" Journal Infection and immunity Remove constraint Journal: Infection and immunity Subject genes Remove constraint Subject: genes
Start Over

Search

76  Search Results

« Previous | 1 - 50 of 76 |
Number of results to display per page

Search Results

1. A Cluster of Four Surface Antigen Genes Specifically Expressed in Bradyzoites, SAG2CDXY, Plays an Important Role in Toxoplasma gondii Persistence

Author:
Saeij, Jeroen P.J.; Arrizabalaga, Gustavo; Boothroyd, John C.
Source:
Infection and immunity 2008 v.76 no.6 pp. 2402-2410
ISSN:
0019-9567
Subject:
parasites,   etc   ; Toxoplasma gondii; antigens; bioluminescence; bradyzoites; brain; genes; image analysis; immune response; luciferase; mice; mutants; pathogenicity; small intestine; tachyzoites;     Show all 15 Subjects
Abstract:
... Toxoplasma gondii is one of the most successful protozoan parasites of warm-blooded animals. Stage-specific expression of its surface molecules is thought to be key to its ability to establish chronic infection in immunocompetent animals. The rapidly dividing tachyzoite stage displays a different subset of family of surface antigen 1 (SAG1)-related sequences (SRSs) from that displayed by the encys ...
DOI:
10.1128/IAI.01494-07
PubMed:
18347037
PubMed Central:
PMC2423105

2. Adherence to Human Vaginal Epithelial Cells Signals for Increased Expression of Trichomonas vaginalis Genes

Author:
Kucknoor, Ashwini S.; Mundodi, Vasanthakrishna; Alderete, J. F.
Source:
Infection and immunity 2005 v.73 no.10 pp. 6472-6478
ISSN:
0019-9567
Subject:
parasites,   etc   ; Trichomonas vaginalis; adhesins; adhesion; cDNA libraries; clones; complementary DNA; epithelial cells; gene expression; gene expression regulation; genes; humans; immunoblotting; iron; monoclonal antibodies; parasitism; reverse transcriptase polymerase chain reaction; transcription (genetics); translation (genetics); vagina;     Show all 20 Subjects
Abstract:
... Host parasitism by Trichomonas vaginalis is complex, and the adhesion to vaginal epithelial cells (VECs) by trichomonads is preparatory to colonization of the vagina. Since we showed increased synthesis of adhesins after contact with VECs (A. F. Garcia, et al., Mol. Microbiol. 47:1207-1224, 2003) and more recently demonstrated up-regulated gene expression in VECs after parasite attachment (A. S. K ...
DOI:
10.1128/IAI.73.10.6472-6478.2005
PubMed:
16177319
PubMed Central:
PMC1230950

3. Alteration in Host Cell Tropism Limits the Efficacy of Immunization with a Surface Protein of Malaria Merozoites

Author:
Shi, Qifang; Cernetich, Amy; Daly, Thomas M.; Galvan, Gina; Vaidya, Akhil B.; Bergman, Lawrence W.; Burns, James M.
Source:
Infection and immunity 2005 v.73 no.10 pp. 6363-6371
ISSN:
0019-9567
Subject:
parasites,   etc   ; Plasmodium yoelii; epitopes; gene expression; gene expression regulation; genes; immunization; malaria; merozoites; mice; microarray technology; neutralization; neutralizing antibodies; protein synthesis; reticulocytes; surface proteins;     Show all 16 Subjects
Abstract:
... Immunization with Plasmodium yoelii merozoite surface protein-8 (PyMSP-8) has been shown to protect mice against lethal P. yoelii 17XL malaria. Here we demonstrate that PyMSP-8-specific antibodies preferentially suppress P. yoelii 17XL growth in mature erythrocytes compared to growth in reticulocytes and do not suppress the growth of nonlethal P. yoelii 17X, a parasite that primarily replicates in ...
DOI:
10.1128/IAI.73.10.6363-6371.2005
PubMed:
16177307
PubMed Central:
PMC1230925

4. An In Vivo and In Vitro Model of Plasmodium falciparum Rosetting and Autoagglutination Mediated by varO, a Group A var Gene Encoding a Frequent Serotype

Author:
Vigan-Womas, Inès; Guillotte, Micheline; Le Scanf, Cécile; Igonet, Sébastien; Petres, Stéphane; Juillerat, Alexandre; Badaut, Cyril; Nato, Farida; Schneider, Achim; Lavergne, Anne; Contamin, Hugues; Tall, Adama; Baril, Laurence; Bentley, Graham A.; Mercereau-Puijalon, Odile
Source:
Infection and immunity 2008 v.76 no.12 pp. 5565-5580
ISSN:
0019-9567
Subject:
parasites,   etc   ; Plasmodium falciparum; Saimiri sciureus; confidence interval; erythrocytes; genes; humans; malaria; mice; models; monkeys; monoclonal antibodies; patients; seroprevalence; serotypes; vaccine development; Senegal;     Show all 17 Subjects
Abstract:
... In the Saimiri sciureus monkey, erythrocytes infected with the varO antigenic variant of the Plasmodium falciparum Palo Alto 89F5 clone bind uninfected red blood cells (rosetting), form autoagglutinates, and have a high multiplication rate, three phenotypic characteristics that are associated with severe malaria in human patients. We report here that varO parasites express a var gene having the ch ...
DOI:
10.1128/IAI.00901-08
PubMed:
18809668
PubMed Central:
PMC2583593

5. A Set of Glycosylphosphatidyl Inositol-Anchored Membrane Proteins of Plasmodium falciparum Is Refractory to Genetic Deletion

Author:
Sanders, Paul R.; Kats, Lev M.; Drew, Damien R.; O'Donnell, Rebecca A.; O'Neill, Matthew; Maier, Alexander G.; Coppel, Ross L.; Crabb, Brendan S.
Source:
Infection and immunity 2006 v.74 no.7 pp. 4330-4338
ISSN:
0019-9567
Subject:
parasites,   etc   ; Plasmodium falciparum; antigens; erythrocytes; gene targeting; genes; membrane proteins; merozoites; organelles; phenotype; receptors; surface proteins;     Show all 12 Subjects
Abstract:
... Targeted gene disruption has proved to be a powerful approach for studying the function of important ligands involved in erythrocyte invasion by the extracellular merozoite form of the human malaria parasite, Plasmodium falciparum. Merozoite invasion proceeds via a number of seemingly independent alternate pathways, such that entry can proceed with parasites lacking particular ligand-receptor inte ...
DOI:
10.1128/IAI.00054-06
PubMed:
16790807
PubMed Central:
PMC1489731

6. A Transmembrane Domain-Containing Surface Protein from Toxoplasma gondii Augments Replication in Activated Immune Cells and Establishment of a Chronic Infection

Author:
Pollard, Angela M.; Skariah, Sini; Mordue, Dana G.; Knoll, Laura J.
Source:
Infection and immunity 2009 v.77 no.9 pp. 3731-3739
ISSN:
0019-9567
Subject:
parasites,   etc   ; Toxoplasma gondii; antigens; blood serum; bradyzoites; fluorescent antibody technique; genes; genetic complementation; macrophages; mice; mutants; nitric oxide; polypeptides; surface proteins;     Show all 14 Subjects
Abstract:
... Toxoplasma gondii mutants identified as defective in the establishment of chronic infection were screened to isolate those specifically impaired in their ability to replicate within activated macrophages. One of the identified mutants contains an insertion in the hypothetical gene TGME49_111670. Genetic complementation restores the ability of the mutant to replicate in immune cells and produce cys ...
DOI:
10.1128/IAI.00450-09
PubMed:
19581395
PubMed Central:
PMC2737990

7. Bioinformatic Identification of Tandem Repeat Antigens of the Leishmania donovani Complex

Author:
Goto, Yasuyuki; Coler, Rhea N.; Reed, Steven G.
Source:
Infection and immunity 2007 v.75 no.2 pp. 846-851
ISSN:
0019-9567
Subject:
parasites,   etc   ; B-lymphocytes; Leishmania donovani; Leishmania infantum; antigens; bioinformatics; databases; genes; humans; nucleotide sequences; patients; proteins; screening; visceral leishmaniasis;     Show all 14 Subjects
Abstract:
... With large amounts of parasite gene sequence available, additional bioinformatic tools to screen these sequences for identifying genes encoding antigens are needed. Proteins containing tandem repeat (TR) domains are often B-cell antigens, and antibody responses toward TR domains of the proteins are dominant in human infected with certain parasites. We hypothesized that antigens of serological sign ...
DOI:
10.1128/IAI.01205-06
PubMed:
17088350
PubMed Central:
PMC1828517

8. Carboxypeptidases B of Anopheles gambiae as Targets for a Plasmodium falciparum Transmission-Blocking Vaccine

Author:
Lavazec, C.; Boudin, C.; Lacroix, R.; Bonnet, S.; Diop, A.; Thiberge, S.; Boisson, B.; Tahar, R.; Bourgouin, C.
Source:
Infection and immunity 2007 v.75 no.4 pp. 1635-1642
ISSN:
0019-9567
Subject:
parasites,   etc   ; Anopheles gambiae; Plasmodium berghei; Plasmodium falciparum; antibodies; antigens; carboxypeptidase B; gene expression regulation; genes; malaria; mice; midgut; reproductive performance; vaccines; Africa;     Show all 15 Subjects
Abstract:
... Anopheles gambiae is the major African vector of Plasmodium falciparum, the most deadly species of human malaria parasite and the most prevalent in Africa. Several strategies are being developed to limit the global impact of malaria via reducing transmission rates, among which are transmission-blocking vaccines (TBVs), which induce in the vertebrate host the production of antibodies that inhibit p ...
DOI:
10.1128/IAI.00864-06
PubMed:
17283100
PubMed Central:
PMC1865713

9. Cell Sorting-Assisted Microarray Profiling of Host Cell Response to Cryptosporidium parvum Infection

Author:
Yang, Yi-Lin; Buck, Gregory A.; Widmer, Giovanni
Source:
Infection and immunity 2010 v.78 no.3 pp. 1040-1048
ISSN:
0019-9567
Subject:
parasites,   etc   ; Cryptosporidium parvum; agglutinins; experimental design; fluorescence; gene expression regulation; genes; glycoproteins; lectins; mammals; metabolism; microarray technology; soybeans; transcription (genetics);     Show all 14 Subjects
Abstract:
... To study the transcriptional response of mammalian cells to infection with the intracellular apicomplexan parasite Cryptosporidium parvum, infected and uninfected cells were recovered from C. parvum-infected cell monolayers. This approach, which contrasts with a more conventional experimental design that compares infected to uninfected cell monolayers, enabled the identification of functional cate ...
DOI:
10.1128/IAI.01009-09
PubMed:
20038534
PubMed Central:
PMC2825902

10. Chlamydial Infection Increases Gonococcal Colonization in a Novel Murine Coinfection Model

Author:
Vonck, Rachel A.; Darville, T.; O'Connell, C.M.; Jerse, Ann E.
Source:
Infection and immunity 2011 v.79 no.4 pp. 1566-1577
ISSN:
0019-9567
Subject:
host-parasite relationships,   etc   ; Chlamydia trachomatis; Neisseria gonorrhoeae; antimicrobial peptides; chemokines; epithelial cells; estradiol; gene expression regulation; genes; immunohistochemistry; macrophages; mice; mixed infection; models; neutrophils; pathogens; serotypes; tumor necrosis factor-alpha;     Show all 18 Subjects
Abstract:
... Genital tract infections caused by Neisseria gonorrhoeae and Chlamydia trachomatis serovars D to K occur at high incidence in many areas of the world. Despite high rates of coinfection with these pathogens, investigations of host-parasite interactions have focused on each pathogen individually. We describe here a coinfection model in which female BALB/c mice were first infected with the mouse Chla ...
DOI:
10.1128/IAI.01155-10
PubMed:
21245268
PubMed Central:
PMC3067530

11. Cloning, Characterization, and Serodiagnostic Evaluation of Leishmania infantum Tandem Repeat Proteins

Author:
Goto, Yasuyuki; Coler, Rhea N.; Guderian, Jeffrey; Mohamath, Raodoh; Reed, Steven G.
Source:
Infection and immunity 2006 v.74 no.7 pp. 3939-3945
ISSN:
0019-9567
Subject:
parasites,   etc   ; B-lymphocytes; Leishmania infantum; databases; enzyme-linked immunosorbent assay; epitopes; genes; hamsters; humans; patients; recombinant antigens; recombinant proteins; screening; serodiagnosis; visceral leishmaniasis;     Show all 15 Subjects
Abstract:
... Visceral leishmaniasis (VL) is a form of leishmaniasis, which is caused by infection with the protozoan parasite Leishmania, and is often fatal unless it is treated. Rapid and accurate diagnosis of VL is important for effective treatment. Here we report the cloning of previously undescribed tandem repeat (TR) proteins of Leishmania infantum and an evaluation of VL patient antibody responses to the ...
DOI:
10.1128/IAI.00101-06
PubMed:
16790767
PubMed Central:
PMC1489730

12. Comparison of the Effects of Leishmania major or Leishmania donovani Infection on Macrophage Gene Expression

Author:
Gregory, David J.; Sladek, Robert; Olivier, Martin; Matlashewski, Greg
Source:
Infection and immunity 2008 v.76 no.3 pp. 1186-1192
ISSN:
0019-9567
Subject:
parasites,   etc   ; Leishmania donovani; Leishmania major; gene expression; genes; human diseases; macrophages; messenger RNA; metalloproteinases; promastigotes; prostaglandins; skin lesions; spermine;     Show all 13 Subjects
Abstract:
... The intracellular parasite Leishmania causes a wide spectrum of human disease, ranging from self-resolving cutaneous lesions to fatal visceral disease, depending on the species of Leishmania involved. The mechanisms by which different Leishmania species cause different pathologies are largely unknown. We have addressed this question by comparing the gene expression profiles of bone marrow-derived ...
DOI:
10.1128/IAI.01320-07
PubMed:
18086813
PubMed Central:
PMC2258831

13. Comparisons of Mutants Lacking the Golgi UDP-Galactose or GDP-Mannose Transporters Establish that Phosphoglycans Are Important for Promastigote but Not Amastigote Virulence in Leishmania major

Author:
Capul, Althea A.; Hickerson, Suzanne; Barron, Tamara; Turco, Salvatore J.; Beverley, Stephen M.
Source:
Infection and immunity 2007 v.75 no.9 pp. 4629-4637
ISSN:
0019-9567
Subject:
parasites,   etc   ; Golgi apparatus; Leishmania major; amastigotes; genes; glycoconjugates; immunity; macrophages; mannans; metabolites; mice; mutants; phenotype; promastigotes; transporters; virulence;     Show all 16 Subjects
Abstract:
... Abundant surface Leishmania phosphoglycans (PGs) containing [Gal(β1,4)Man(α1-PO₄)]-derived repeating units are important at several points in the infectious cycle of this protozoan parasite. PG synthesis requires transport of activated nucleotide-sugar precursors from the cytoplasm to the Golgi apparatus. Correspondingly, null mutants of the L. major GDP-mannose transporter LPG2 lack PGs and are s ...
DOI:
10.1128/IAI.00735-07
PubMed:
17606605
PubMed Central:
PMC1951182

14. Cross-Reactive Surface Epitopes on Chondroitin Sulfate A-Adherent Plasmodium falciparum-Infected Erythrocytes Are Associated with Transcription of var2csa

Author:
Elliott, Salenna R.; Duffy, Michael F.; Byrne, Timothy J.; Beeson, James G.; Mann, Emily J.; Wilson, Danny W.; Rogerson, Stephen J.; Brown, Graham V.
Source:
Infection and immunity 2005 v.73 no.5 pp. 2848-2856
ISSN:
0019-9567
Subject:
parasites,   etc   ; Plasmodium falciparum; adhesion; antiserum; chondroitin sulfate; epitopes; erythrocytes; flow cytometry; genes; malaria; membrane proteins; pregnancy; transcription (genetics);     Show all 13 Subjects
Abstract:
... Malaria in pregnancy is associated with placental accumulation of Plasmodium falciparum-infected erythrocytes (IE) that adhere to chondroitin sulfate A (CSA). Adhesion is mediated by P. falciparum erythrocyte membrane protein 1 (PfEMP1), a variant parasite protein expressed on the surface of IE and encoded by var genes. Rabbit antiserum was generated against the CSA-adherent P. falciparum line CS2 ...
DOI:
10.1128/IAI.73.5.2848-2856.2005
PubMed:
15845490
PubMed Central:
PMC1087379

15. Cytokines, Signaling Pathways, and Effector Molecules Required for the Control of Leishmania (Viannia) braziliensis in Mice

Author:
Rocha, F. Janaina Soares; Schleicher, Ulrike; Mattner, Jochen; Alber, Gottfried; Bogdan, Christian
Source:
Infection and immunity 2007 v.75 no.8 pp. 3823-3832
ISSN:
0019-9567
Subject:
parasites,   etc   ; Leishmania braziliensis; Leishmania major; NADP (coenzyme); animal models; cutaneous leishmaniasis; disease course; genes; immune response; interleukin-12; mice; nitric oxide synthase; pathogens; signal transduction; skin lesions; spleen; transcription factors; tumor necrosis factors; visceral leishmaniasis;     Show all 19 Subjects
Abstract:
... Cutaneous leishmaniasis is caused by protozoan parasites of the genus LEISHMANIA: The mechanisms of pathogen control have been established primarily in the mouse model of Leishmania major infection, but they might not hold true for other Leishmania species associated with cutaneous disease. Here, we analyzed the role of cytokines, signaling components, and effector molecules in the control of New ...
DOI:
10.1128/IAI.01335-06
PubMed:
17517868
PubMed Central:
PMC1951993

16. Developmental Stage- and Cell Cycle Number-Dependent Changes in Characteristics of Plasmodium falciparum-Infected Erythrocyte Adherence to Placental Chondroitin-4-Sulfate Proteoglycan

Author:
Madhunapantula, SubbaRao V.; Achur, Rajeshwara N.; Gowda, D. Channe
Source:
Infection and immunity 2007 v.75 no.9 pp. 4409-4415
ISSN:
0019-9567
Subject:
parasites,   etc   ; Plasmodium; binding capacity; cell cycle; erythrocytes; genes; humans; placenta; protein binding; protein synthesis; proteoglycans; trophozoites; trypsin;     Show all 13 Subjects
Abstract:
... The adherence of Plasmodium falciparum-infected red blood cells (IRBCs) in the human placenta is mediated by chondroitin-4-sulfate (C4S). Although IRBC binding to C4S has been unequivocally established, the adherence characteristics of IRBCs at different stages of parasite development and through successive parasite generations after selection for C4S adherence are not known. Here we show that IRB ...
DOI:
10.1128/IAI.00478-07
PubMed:
17591790
PubMed Central:
PMC1951145

17. Differential Microbicidal Effects of Human Histone Proteins H2A and H2B on Leishmania Promastigotes and Amastigotes

Author:
Wang, Yingwei; Chen, Yang; Xin, Lijun; Beverley, Stephen M.; Carlsen, Eric D.; Popov, Vsevolod; Chang, Kwang-Poo; Wang, Ming; Soong, Lynn
Source:
Infection and immunity 2011 v.79 no.3 pp. 1124-1133
ISSN:
0019-9567
Subject:
parasites,   etc   ; Leishmania amazonensis; amastigotes; antimicrobial peptides; bacteria; fungi; genes; histones; humans; innate immunity; macrophages; mice; pathogenicity; promastigotes; scanning electron microscopy;     Show all 15 Subjects
Abstract:
... Recent studies have shown that histone proteins can act as antimicrobial peptides in host defense against extracellular bacteria, fungi, and Leishmania promastigotes. In this study, we used human recombinant histone proteins to further study their leishmaniacidal effects and the underlying mechanisms. We found that the histones H2A and H2B (but not H1⁰) could directly and efficiently kill promasti ...
DOI:
10.1128/IAI.00658-10
PubMed:
21189319
PubMed Central:
PMC3067510

18. Direct Activation of Human Endothelial Cells by Plasmodium falciparum-Infected Erythrocytes

Author:
Viebig, Nicola K.; Wulbrand, Ulrich; Förster, Reinhold; Andrews, Katherine T.; Lanzer, Michael; Knolle, Percy A.
Source:
Infection and immunity 2005 v.73 no.6 pp. 3271-3277
ISSN:
0019-9567
Subject:
parasites,   etc   ; Plasmodium falciparum; adhesion; cell adhesion; chemokines; endothelial cells; erythrocytes; gene expression; genes; humans; hypoxia; immune response; macrophages; parasitemia; tumor necrosis factor-alpha;     Show all 15 Subjects
Abstract:
... Cytoadherence of Plasmodium falciparum-infected erythrocytes (PRBC) to endothelial cells causes severe clinical disease, presumably as a of result perfusion failure and tissue hypoxia. Cytoadherence to endothelial cells is increased by endothelial cell activation, which is believed to occur in a paracrine fashion by mediators such as tumor necrosis factor alpha (TNF-[alpha]) released from macropha ...
DOI:
10.1128/IAI.73.6.3271-3277.2005
PubMed:
15908351
PubMed Central:
PMC1111820

19. Disruption of a Locus Encoding a Nucleolar Zinc Finger Protein Decreases Tachyzoite-to-Bradyzoite Differentiation in Toxoplasma gondii

Author:
Vanchinathan, Padmini; Brewer, Jeremy L.; Harb, Omar S.; Boothroyd, John C.; Singh, Upinder
Source:
Infection and immunity 2005 v.73 no.10 pp. 6680-6688
ISSN:
0019-9567
Subject:
parasites,   etc   ; Toxoplasma gondii; bradyzoites; cell nucleolus; genes; insertional mutagenesis; intermediate hosts; loci; mutants; pathogenicity; phenotype; process control; tachyzoites; toxicity; zinc finger motif;     Show all 15 Subjects
Abstract:
... During its life cycle in intermediate hosts, Toxoplasma gondii exists in two interconverting developmental stages: tachyzoites and bradyzoites. This interconversion is essential for the survival and pathogenicity of the parasite, but little is known about the genetic mechanisms that control this process. We have previously generated tachyzoite-to-bradyzoite differentiation (Tbd⁻) mutants using che ...
DOI:
10.1128/IAI.73.10.6680-6688.2005
PubMed:
16177345
PubMed Central:
PMC1230886

20. Environmental Mimics and the Lvh Type IVA Secretion System Contribute to Virulence-Related Phenotypes of Legionella pneumophila

Author:
Bandyopadhyay, Purnima; Liu, Shuqing; Gabbai, Carolina B.; Venitelli, Zeah; Steinman, Howard M.
Source:
Infection and immunity 2007 v.75 no.2 pp. 723-735
ISSN:
0019-9567
Subject:
parasites,   etc   ; Acanthamoeba castellanii; Agrobacterium radiobacter; Legionella pneumophila; acidification; bacteria; encystment; genes; loci; macrophages; models; mutants; niches; pathogens; phagosomes; phenotype; secretion; trophozoites; virulence;     Show all 19 Subjects
Abstract:
... Legionella pneumophila, the causative organism of Legionnaires' disease, is a fresh-water bacterium and intracellular parasite of amoebae. This study examined the effects of incubation in water and amoeba encystment on L. pneumophila strain JR32 and null mutants in dot/icm genes encoding a type IVB secretion system required for entry, delayed acidification of L. pneumophila-containing phagosomes, ...
DOI:
10.1128/IAI.00956-06
PubMed:
17101653
PubMed Central:
PMC1828514

21. Evaluation of Three Pichia pastoris-Expressed Plasmodium falciparum Merozoite Proteins as a Combination Vaccine against Infection with Blood-Stage Parasites

Author:
Zhang, Dongmei; Pan, Weiqing
Source:
Infection and immunity 2005 v.73 no.10 pp. 6530-6536
ISSN:
0019-9567
Subject:
parasites,   etc   ; rabbits; merozoites; Komagataella pastoris; genes; malaria; immunoglobulin G; trypsin; antibodies; monkeys; patients; antigens; humans; yeasts; mice; immune response; immunization; Plasmodium falciparum; Macaca mulatta; erythrocytes; animal models; sialidase;     Show all 22 Subjects
Abstract:
... Because invasion of erythrocytes by Plasmodium falciparum merozoites involves multiple receptor-ligand interactions, it may be necessary to develop a multivalent malaria vaccine that is comprised of distinct parasite ligands. PfAMA-1, PfMSP1, and PfEBA-175 are merozoite proteins that play important roles in invasion. We have constructed a PfCP-2.9 chimeric protein consisting of PfAMA-1 and PfMSP1 ...
DOI:
10.1128/IAI.73.10.6530-6536.2005
PubMed:
16177327
PubMed Central:
PMC1230943

22. Evidence for a Common Role for the Serine-Type Plasmodium falciparum Serine Repeat Antigen Proteases: Implications for Vaccine and Drug Design

Author:
McCoubrie, Joanne E.; Miller, Susanne K.; Sargeant, Tobias; Good, Robert T.; Hodder, Anthony N.; Speed, Terence P.; de Koning-Ward, Tania F.; Crabb, Brendan S.
Source:
Infection and immunity 2007 v.75 no.12 pp. 5565-5574
ISSN:
0019-9567
Subject:
parasites,   etc   ; Plasmodium falciparum; antigens; cysteine; drugs; genes; humans; malaria; messenger RNA; phylogeny; proteinases; serine; vaccine development;     Show all 13 Subjects
Abstract:
... Serine repeat antigens (SERAs) are a family of secreted "cysteine-like" proteases of Plasmodium parasites. Several SERAs possess an atypical active-site serine residue in place of the canonical cysteine. The human malaria parasite Plasmodium falciparum possesses six "serine-type" (SERA1 to SERA5 and SERA9) and three "cysteine-type" (SERA6 to SERA8) SERAs. Here, we investigate the importance of the ...
DOI:
10.1128/IAI.00405-07
PubMed:
17893128
PubMed Central:
PMC2168336

23. Expression and Function of a Family of Transmembrane Kinases from the Protozoan Parasite Entamoeba histolytica

Author:
Mehra, Alka; Fredrick, Jesse; Petri, William A. Jr.; Bhattacharya, Sudha; Bhattacharya, Alok
Source:
Infection and immunity 2006 v.74 no.9 pp. 5341-5351
ISSN:
0019-9567
Subject:
parasites,   etc   ; proteome; trophozoites; blood serum; eukaryotic cells; Entamoeba histolytica; genes; genomics; phosphotransferases (kinases); antiserum; cell proliferation; starvation;     Show all 12 Subjects
Abstract:
... The signaling proteome of Entamoeba histolytica is made of transmembrane kinases (TMKs) that are rarely found in unicellular eukaryotes. There are 90 TMK genes reported for E. histolytica, and these have been grouped into nine distinct families based on motifs present on both extracellular and kinase domains. Of these, the B1 family was chosen for further analysis. Genomic sequencing revealed the ...
DOI:
10.1128/IAI.00025-06
PubMed:
16926429
PubMed Central:
PMC1594846

24. Generation of Trypanosoma cruzi-Specific CD8⁺ T-Cell Immunity Is Unaffected by the Absence of Type I Interferon Signaling

Author:
Martin, Diana L.; Murali-Krishna, Kaja; Tarleton, Rick L.
Source:
Infection and immunity 2010 v.78 no.7 pp. 3154-3159
ISSN:
0019-9567
Subject:
parasites,   etc   ; CD8-positive T-lymphocytes; Chagas disease; Trypanosoma cruzi; Vaccinia virus; adaptive immunity; cytokines; genes; heart failure; humans; interferons; mice; models; pathogens; South America;     Show all 15 Subjects
Abstract:
... Trypanosoma cruzi is a protozoan parasite that causes human Chagas' disease, a leading source of congestive heart failure in Central and South America. CD8⁺ T cells are critical for control of T. cruzi infection, and CD8⁺ T cells recognizing the immunodominant trans-sialidase gene-encoded peptide TSKB20 (ANYKFTLV) account for approximately 30% of the total CD8⁺ T-cell population at the peak of inf ...
DOI:
10.1128/IAI.00275-10
PubMed:
20457790
PubMed Central:
PMC2897408

25. Giardia lamblia-Induced Changes in Gene Expression in Differentiated Caco-2 Human Intestinal Epithelial Cells

Author:
Roxström-Lindquist, Katarina; Ringqvist, Emma; Palm, Daniel; Svärd, Staffan
Source:
Infection and immunity 2005 v.73 no.12 pp. 8204-8208
ISSN:
0019-9567
Subject:
parasites,   etc   ; Giardia lamblia; adaptive immunity; chemokines; diarrhea; epithelial cells; gene expression regulation; genes; human cell lines; humans; inflammation; intestinal mucosa; microarray technology; pathogenesis; pathogens; stress response; transcriptome;     Show all 17 Subjects
Abstract:
... The parasitic protozoan Giardia lamblia is a worldwide cause of diarrhea, but the mechanism of disease remains elusive. The parasite colonizes the small intestinal epithelium, known to be a sensor for the presence of enteric pathogens, without invading or causing severe inflammation. In this study we investigated the epithelial cell response to G. lamblia. Differentiated Caco-2 cells were infected ...
DOI:
10.1128/IAI.73.12.8204-8208.2005
PubMed:
16299316
PubMed Central:
PMC1307045

26. Hierarchical, Domain Type-Specific Acquisition of Antibodies to Plasmodium falciparum Erythrocyte Membrane Protein 1 in Tanzanian Children

Author:
Cham, Gerald K.K.; Turner, Louise; Kurtis, Jonathan D.; Mutabingwa, Theonest; Fried, Michal; Jensen, Anja T.R.; Lavstsen, Thomas; Hviid, Lars; Duffy, Patrick E.; Theander, Thor G.
Source:
Infection and immunity 2010 v.78 no.11 pp. 4653-4659
ISSN:
0019-9567
Subject:
parasites,   etc   ; Plasmodium falciparum; antibodies; antigens; children; erythrocytes; filtration; genes; indigenous species; longitudinal studies; malaria; membrane proteins; spleen;     Show all 13 Subjects
Abstract:
... Plasmodium falciparum erythrocyte membrane protein 1 (PfEMP1) is a variant antigen expressed on the surface of malaria-infected erythrocytes. PfEMP1 attaches to the vascular lining and allows infected erythrocytes to avoid filtration through the spleen. Each parasite genome encodes about 60 different PfEMP1 variants, each PfEMP1 comprises several domains in its extracellular region, and the PfEMP1 ...
DOI:
10.1128/IAI.00593-10
PubMed:
20823214
PubMed Central:
PMC2976311

27. Human Recombinant Antibodies against Plasmodium falciparum Merozoite Surface Protein 3 Cloned from Peripheral Blood Leukocytes of Individuals with Immunity to Malaria Demonstrate Antiparasitic Properties

Author:
Lundquist, Rasmus; Nielsen, Leif Kofoed; Jafarshad, Ali; SoeSoe, Daw; Christensen, Lars Harder; Druilhe, Pierre; Dziegiel, Morten Hanefeld
Source:
Infection and immunity 2006 v.74 no.6 pp. 3222-3231
ISSN:
0019-9567
Subject:
parasites,   etc   ; Plasmodium falciparum; antigens; antiparasitic agents; flow cytometry; genes; humans; immunity; immunoblotting; immunoglobulins; leukocytes; malaria; merozoites; messenger RNA; patients; recombinant antibodies; surface proteins;     Show all 17 Subjects
Abstract:
... Immunoglobulinsfrom individuals with immunity to malaria have a strong antiparasiticeffect when transferred to Plasmodium falciparum malariainfected patients. One prominent target of antiparasitic antibodies isthe merozoite surface antigen 3 (MSP-3). We have investigated theantibody response against MSP-3 residues 194 to 257(MSP-3₁₉₄₋₂₅₇) on the molecular level. mRNA fromperipheral blood leukocyte ...
DOI:
10.1128/IAI.00928-05
PubMed:
16714549
PubMed Central:
PMC1479282

28. Identification, Cloning, Expression, and Characterization of the Gene for Plasmodium knowlesi Surface Protein Containing an Altered Thrombospondin Repeat Domain

Author:
Mahajan, Babita; Jani, Dewal; Chattopadhyay, Rana; Nagarkatti, Rana; Zheng, Hong; Majam, Victoria; Weiss, Walter; Kumar, Sanjai; Rathore, Dharmendar
Source:
Infection and immunity 2005 v.73 no.9 pp. 5402-5409
ISSN:
0019-9567
Subject:
parasites,   etc   ; DNA; Escherichia coli; Macaca mulatta; Plasmodium falciparum; Plasmodium knowlesi; Plasmodium yoelii; adhesion; antigens; erythrocytic stage; fluorescent antibody technique; genes; hepatocytes; hepatoma; humans; malaria; models; monkeys; nucleotide sequences; proteinases; sequence analysis; surface proteins;     Show all 22 Subjects
Abstract:
... Proteins present on the surface of malaria parasites that participate in the process of invasion and adhesion to host cells are considered attractive vaccine targets. Aided by the availability of the partially completed genome sequence of the simian malaria parasite Plasmodium knowlesi, we have identified a 786-bp DNA sequence that encodes a 262-amino-acid-long protein, containing an altered versi ...
DOI:
10.1128/IAI.73.9.5402-5409.2005
PubMed:
16113256
PubMed Central:
PMC1231135

29. Identification of a Novel Antigen of Schistosoma mansoni Shared with Plasmodium falciparum and Evaluation of Different Cross-Reactive Antibody Subclasses Induced by Human Schistosomiasis and Malaria

Author:
Pierrot, Christine; Wilson, Shona; Lallet, Hélène; Lafitte, Sophia; Jones, Frances M.; Daher, Wassim; Capron, Monique; Dunne, David W.; Khalife, Jamal
Source:
Infection and immunity 2006 v.74 no.6 pp. 3347-3354
ISSN:
0019-9567
Subject:
parasites,   etc   ; Plasmodium falciparum; correlation; schistosomiasis; antigens; humans; Schistosoma mansoni; antibodies; immunoglobulins; genes; malaria; Kenya; Uganda;     Show all 13 Subjects
Abstract:
... Plasmodium falciparum and Schistosoma mansoni are often found in human coinfections, and cross-reactive antibodies to different components of the two parasites have been detected. In this work, we identified a cross-reactive S. mansoni gene product, referred to as SmLRR, that seems to belong to the leucine-rich repeat protein family. Comparative analysis of SmLRR revealed 57% similarity with a put ...
DOI:
10.1128/IAI.01724-05
PubMed:
16714563
PubMed Central:
PMC1479256

30. Identification of Differentially Expressed Genes in Virulent and Nonvirulent Entamoeba Species: Potential Implications for Amebic Pathogenesis

Author:
MacFarlane, Ryan C.; Singh, Upinder
Source:
Infection and immunity 2006 v.74 no.1 pp. 340-351
ISSN:
0019-9567
Subject:
parasites,   etc   ; Entamoeba histolytica; colitis; evolution; ferredoxin hydrogenase; gene expression; gene expression regulation; genes; hemagglutinins; iron; liver abscess; lysozyme; microarray technology; pathogenesis; peroxiredoxin; phenotype; retrotransposons; stress response; transcription (genetics); virulence; virulent strains;     Show all 21 Subjects
Abstract:
... Entamoeba histolytica is a protozoan parasite that causes colitis and liver abscesses. Several Entamoeba species and strains with differing levels of virulence have been identified. E. histolytica HM-1:IMSS is a virulent strain, E. histolytica Rahman is a nonvirulent strain, and Entamoeba dispar is a nonvirulent species. We used an E. histolytica DNA microarray consisting of 2,110 genes to assess ...
DOI:
10.1128/IAI.74.1.340-351.2006
PubMed:
16368989
PubMed Central:
PMC1346599

31. Identification of Novel Genes in Intestinal Tissue That Are Regulated after Infection with an Intestinal Nematode Parasite

Author:
Datta, R.; deSchoolmeester, M. L.; Hedeler, C.; Paton, N. W.; Brass, A. M.; Else, K. J.
Source:
Infection and immunity 2005 v.73 no.7 pp. 4025-4033
ISSN:
0019-9567
Subject:
parasites,   etc   ; Trichuris muris; gene expression; gene expression regulation; genes; interferon-gamma; intestines; lymph nodes; metabolism; mice; microarray technology; mucosa; transfer RNA; tryptophan;     Show all 14 Subjects
Abstract:
... Infection of resistant or susceptible mice with Trichuris muris provokes mesenteric lymph node responses which are polarized towards Th2 or Th1, respectively. These responses are well documented in the literature. In contrast, little is known about the local responses occurring within the infected intestine. Through microarray analyses, we demonstrate that the gene expression profile of infected g ...
DOI:
10.1128/IAI.73.7.4025-4033.2005
PubMed:
15972490
PubMed Central:
PMC1168561

32. Immunoglobulin G Antibody Reactivity to a Group A Plasmodium falciparum Erythrocyte Membrane Protein 1 and Protection from P. falciparum Malaria

Author:
Magistrado, Pamela A.; Lusingu, John; Vestergaard, Lasse S.; Lemnge, Martha; Lavstsen, Thomas; Turner, Louise; Hviid, Lars; Jensen, Anja T.R.; Theander, Thor G.
Source:
Infection and immunity 2007 v.75 no.5 pp. 2415-2420
ISSN:
0019-9567
Subject:
parasites,   etc   ; Plasmodium falciparum; adaptive immunity; antibodies; children; erythrocytes; genes; immunoglobulin G; malaria; membrane proteins; pathogenesis; surface antigens;     Show all 12 Subjects
Abstract:
... Variant surface antigens (VSA) on the surface of Plasmodium falciparum-infected red blood cells play a major role in the pathogenesis of malaria and are key targets for acquired immunity. The best-characterized VSA belong to the P. falciparum erythrocyte membrane protein 1 (PfEMP1) family. In areas where P. falciparum is endemic, parasites causing severe malaria and malaria in young children with ...
DOI:
10.1128/IAI.00951-06
PubMed:
17283085
PubMed Central:
PMC1865733

33. Immunological Dominance of Trypanosoma cruzi Tandem Repeat Proteins

Author:
Goto, Yasuyuki; Carter, Darrick; Reed, Steven G.
Source:
Infection and immunity 2008 v.76 no.9 pp. 3967-3974
ISSN:
0019-9567
Subject:
parasites,   etc   ; B-lymphocytes; Chagas disease; Trypanosoma cruzi; antigens; bioinformatics; consensus sequence; genes; immune response; pathogenicity; proteins; screening; sequence homology; signal peptide; surveys;     Show all 15 Subjects
Abstract:
... Proteins with tandem repeat (TR) domains have been found in various protozoan parasites, often acting as targets of B-cell responses. However, the extent of the repeats within Trypanosoma cruzi, the causative agent of Chagas' disease, has not been examined well. Here, we present a systematic survey of the TR genes found in T. cruzi, in comparison with other organisms. Although the characteristics ...
DOI:
10.1128/IAI.00604-08
PubMed:
18625739
PubMed Central:
PMC2519453

34. Infection with Toxoplasma gondii Bradyzoites Has a Diminished Impact on Host Transcript Levels Relative to Tachyzoite Infection

Author:
Fouts, A.E.; Boothroyd, J.C.
Source:
Infection and immunity 2007 v.75 no.2 pp. 634-642
ISSN:
0019-9567
Subject:
parasites,   etc   ; Toxoplasma gondii; animals; bradyzoites; chemokines; extracellular matrix; genes; granulocyte-macrophage colony-stimulating factor; hosts; immune system; microarray technology; morbidity; pathogens; tachyzoites; transcription (genetics);     Show all 15 Subjects
Abstract:
... Toxoplasma gondii, an intracellular pathogen, has the potential to infect nearly every warm-blooded animal but rarely causes morbidity. The ability for the parasite to convert to the bradyzoite stage and live inside slow-growing cysts that can go unnoticed by the host immune system allows for parasite persistence for the life of the infected host. This intracellular survival likely necessitates ho ...
DOI:
10.1128/IAI.01228-06
PubMed:
17088349
PubMed Central:
PMC1828502

35. Inhibition of Apoptosis in Cryptosporidium parvum-Infected Intestinal Epithelial Cells Is Dependent on Survivin

Author:
Liu, Jin; Enomoto, Shinichiro; Lancto, Cheryl A.; Abrahamsen, Mitchell S.; Rutherford, Mark S.
Source:
Infection and immunity 2008 v.76 no.8 pp. 3784-3792
ISSN:
0019-9567
Subject:
parasites,   etc   ; Cryptosporidium parvum; apoptosis; caspase-2; epithelial cells; gene expression; gene expression regulation; genes; humans; intestinal mucosa; proteins; ribosomal RNA;     Show all 12 Subjects
Abstract:
... Cryptosporidium parvum is an obligate intracellular protozoan capable of causing severe diarrheal disease in a wide variety of mammals, including humans. C. parvum infection has been associated with induction of apoptosis in exposed epithelial cells, and we now demonstrate that apoptosis is restricted to a subset of cells actively infected with C. parvum. Approximately 20% of the infected cells un ...
DOI:
10.1128/IAI.00308-08
PubMed:
18519556
PubMed Central:
PMC2493198

36. Inhibition of Malaria Parasite Development by a Cyclic Peptide That Targets the Vital Parasite Protein SERA5

Author:
Fairlie, W. Douglas; Spurck, Tim P.; McCoubrie, Joanne E.; Gilson, Paul R.; Miller, Susanne K.; McFadden, Geoffrey I.; Malby, Robyn; Crabb, Brendan S.; Hodder, Anthony N.
Source:
Infection and immunity 2008 v.76 no.9 pp. 4332-4344
ISSN:
0019-9567
Subject:
parasites,   etc   ; Plasmodium falciparum; antigens; bacteriophages; cytosol; drugs; erythrocytes; genes; malaria; papain; plasma membrane; proteins; serine; vacuoles;     Show all 14 Subjects
Abstract:
... The serine repeat antigen (SERA) proteins of the malaria parasites Plasmodium spp. contain a putative enzyme domain similar to that of papain family cysteine proteases. In Plasmodium falciparum parasites, more than half of the SERA family proteins, including the most abundantly expressed form, SERA5, have a cysteine-to-serine substitution within the putative catalytic triad of the active site. Alt ...
DOI:
10.1128/IAI.00278-08
PubMed:
18591232
PubMed Central:
PMC2519404

37. Innate Immune Responses to Lung-Stage Helminth Infection Induce Alternatively Activated Alveolar Macrophages

Author:
Reece, Joshua J.; Siracusa, Mark C.; Scott, Alan L.
Source:
Infection and immunity 2006 v.74 no.9 pp. 4970-4981
ISSN:
0019-9567
Subject:
host-parasite relationships,   etc   ; Nippostrongylus brasiliensis; T-lymphocytes; gene expression; gene expression regulation; genes; helminthiasis; hookworms; immune response; immunohistochemistry; inflammation; innate immunity; lungs; macrophages; mice; microarray technology; pneumonia; severe combined immunodeficiency;     Show all 18 Subjects
Abstract:
... While it is well established that infection with the rodent hookworm Nippostrongylus brasiliensis induces a strongly polarized Th2 immune response, little is known about the innate host-parasite interactions that lead to the development of this robust Th2 immunity. We exploited the transient pulmonary phase of N. brasiliensis development to study the innate immune responses induced by this helmint ...
DOI:
10.1128/IAI.00687-06
PubMed:
16926388
PubMed Central:
PMC1594865

38. Intranasal immunization with recombinant Ascaris suum 14-kilodalton antigen coupled with cholera toxin B subunit induces protective immunity to A. suum infection in mice

Author:
Tsuji, N.; Suzuki, K.; Kasuga-Aoki, H.; Matsumoto, Y.; Arakawa, T.; Ishiwata, K.; Isobe, T.
Source:
Infection and immunity 2001 v.69 no.12 pp. 7285-7292
ISSN:
0019-9567
Subject:
parasites,   etc   ; Ascaris suum; Caenorhabditis elegans; Escherichia; amino acids; antibodies; antigens; ascariasis; blood serum; cholera toxin; complementary DNA; dogs; genes; humans; immune response; immunization; immunoglobulin A; immunoglobulin E; immunoglobulin G; intranasal administration; laboratory animals; larvae; mice; recombinant proteins; vaccines;     Show all 25 Subjects
Abstract:
... Animals can be rendered immune to Ascaris parasites by immunization with infectious-stage larvae. The specific parasite gene products that mediate protective responses in ascariasis are unknown. We have identified a cDNA encoding Ascaris suum 14-kDa antigen (As14) and evaluated the vaccinal effect of the Escherichia coli-expressed recombinant protein (rAs14). GenBank analysis showed that As14 has ...
DOI:
10.1128/IAI.69.12.7285-7292.2001
PubMed:
11705899
PubMed Central:
PMC98813

39. Inverse Associations of Human Leukocyte Antigen and Malaria Parasite Types in Two West African Populations

Author:
Young, Karen; Frodsham, Angela; Doumbo, Ogobara K.; Gupta, Sunetra; Dolo, Amagana; Hu, Jiang Ting; Robson, Kathryn J. H.; Crisanti, Andrea; Hill, Adrian V. S.; Gilbert, Sarah C.
Source:
Infection and immunity 2005 v.73 no.2 pp. 953-955
ISSN:
0019-9567
Subject:
parasites,   etc   ; Africans; antigens; genes; genotype; humans; immune response; mathematical models; population genetics; prediction;     Show all 10 Subjects
Abstract:
... Differences in allelic associations between populations continue to cause difficulties in the mapping and identification of susceptibility genes for complex polygenic diseases. Although well recognized, the basis of such interpopulation differences is poorly understood. We present an example of an inverse allelic association of an immune response genotype to an infectious disease in two neighborin ...
DOI:
10.1128/IAI.73.2.953-955.2005
PubMed:
15664937
PubMed Central:
PMC546931

40. L- Arginine and Cationic Amino Acid Transporter 2B Regulate Growth and Survival of Leishmania amazonensis Amastigotes in Macrophages

Author:
Wanasen, Nanchaya; MacLeod, Carol L.; Ellies, Lesley G.; Soong, Lynn
Source:
Infection and immunity 2007 v.75 no.6 pp. 2802-2810
ISSN:
0019-9567
Subject:
parasites,   etc   ; Leishmania amazonensis; amastigotes; amino acid transporters; arginase; gene expression; genes; interferon-gamma; macrophages; messenger RNA; mice; pathogens;     Show all 12 Subjects
Abstract:
... Leishmania spp. are obligate intracellular parasites, requiring a suitable microenvironment for their growth within host cells. We previously reported that the growth of Leishmania amazonensis amastigotes in murine macrophages (Mφs) was enhanced in the presence of gamma interferon (IFN-γ), a Th1 cytokine normally associated with classical Mφ activation and killing of intracellular pathogens. In th ...
DOI:
10.1128/IAI.00026-07
PubMed:
17387163
PubMed Central:
PMC1932894

41. Leishmania donovani Ornithine Decarboxylase Is Indispensable for Parasite Survival in the Mammalian Host

Author:
Boitz, Jan M.; Yates, Phillip A.; Kline, Chelsey; Gaur, Upasna; Wilson, Mary E.; Ullman, Buddy; Roberts, Sigrid C.
Source:
Infection and immunity 2009 v.77 no.2 pp. 756-763
ISSN:
0019-9567
Subject:
parasites,   etc   ; Leishmania donovani; alpha-difluoromethylornithine; amastigotes; biosynthesis; culture media; enzyme inhibitors; etiological agents; genes; insect vectors; macrophages; mice; mutants; mutation; ornithine decarboxylase; pathogenicity; phenotype; polyamines; promastigotes; putrescine; viability; virulent strains; visceral leishmaniasis;     Show all 23 Subjects
Abstract:
... Mutations within the polyamine biosynthetic pathway of Leishmania donovani, the etiological agent of visceral leishmaniasis, confer polyamine auxotrophy to the insect vector or promastigote form of the parasite. However, whether the infectious or amastigote form of the parasite requires an intact polyamine pathway has remained an open question. To address this issue, conditionally lethal Δodc muta ...

42. Leishmania donovani Ornithine Decarboxylase Is Indispensable for Parasite Survival in the Mammalian Host

Author:
Boitz, Jan M.; Yates, Phillip A.; Kline, Chelsey; Gaur, Upasna; Wilson, Mary E.; Ullman, Buddy; Roberts, Sigrid C.
Source:
Infection and immunity 2009 v.77 no.2 pp. 756-763
ISSN:
0019-9567
Subject:
parasite load; parasites,   etc   ; Leishmania donovani; alpha-difluoromethylornithine; amastigotes; auxotrophs; biochemical pathways; biosynthesis; culture media; enzyme inhibitors; etiological agents; genes; insect vectors; liver; macrophages; mice; mutation; ornithine decarboxylase; pathogenicity; phenotype; polyamines; promastigotes; putrescine; spleen; therapeutics; viability; virulent strains; visceral leishmaniasis;     Show all 28 Subjects
Abstract:
... Mutations within the polyamine biosynthetic pathway of Leishmania donovani, the etiological agent of visceral leishmaniasis, confer polyamine auxotrophy to the insect vector or promastigote form of the parasite. However, whether the infectious or amastigote form of the parasite requires an intact polyamine pathway has remained an open question. To address this issue, conditionally lethal Δodc muta ...
DOI:
10.1128/IAI.01236-08

43. Leishmania major Abrogates Gamma Interferon-Induced Gene Expression in Human Macrophages from a Global Perspective

Author:
Dogra, Nisha; Warburton, Corinna; McMaster, W. Robert
Source:
Infection and immunity 2007 v.75 no.7 pp. 3506-3515
ISSN:
0019-9567
Subject:
parasites,   etc   ; Leishmania major; cell adhesion; gene expression; genes; humans; innate immunity; interferon-gamma; macrophages; microarray technology; statistical analysis; virulence;     Show all 12 Subjects
Abstract:
... Infection with Leishmania major triggers several pathways in the host cell that are crucial to initial infection as well as those that are used by Leishmania to enhance its replication and virulence. To identify the molecular events of the host cell in response to Leishmania, the global gene expression of the human monocytic cell line THP-1 either infected with Leishmania major in the presence and ...
DOI:
10.1128/IAI.00277-07
PubMed:
17470541
PubMed Central:
PMC1932916

44. Localization and Developmental Regulation of a Dispersed Gene Family 1 Protein in Trypanosoma cruzi

Author:
Lander, Noelia; Bernal, Carolina; Diez, Nardy; Añez, Néstor; Docampo, Roberto; Ramírez, José Luis
Source:
Infection and immunity 2010 v.78 no.1 pp. 231-240
ISSN:
0019-9567
Subject:
parasites,   etc   ; Trypanosoma cruzi; Western blotting; antibodies; culture media; droplets; epimastigotes; fluorescent antibody technique; genes; mass spectrometry; microbodies; molecular weight; phosphorylation; plasma membrane; protein products; secretion; starvation; trypomastigotes;     Show all 18 Subjects
Abstract:
... The dispersed gene family 1 (DGF-1) is the fifth largest gene family in the Trypanosoma cruzi genome, with over 500 members (11). Many of the predicted DGF-1 protein products have several transmembrane domains and N-glycosylation and phosphorylation sites and were thought to localize in the plasma membrane. Here, we report that affinity-purified antibodies against a region of one of these proteins ...
DOI:
10.1128/IAI.00780-09
PubMed:
19841080
PubMed Central:
PMC2798230

45. Modulation of Early β-Defensin-2 Production as a Mechanism Developed by Type I Toxoplasma gondii To Evade Human Intestinal Immunity

Author:
Morampudi, Vijay; Braun, Michel Y.; D'Souza, Sushila
Source:
Infection and immunity 2011 v.79 no.5 pp. 2043-2050
ISSN:
0019-9567
Subject:
parasites,   etc   ; Toxoplasma gondii; gene activation; gene expression; genes; genotype; humans; immune evasion; innate immunity; intestinal mucosa; pathogenicity; transcriptomics; viability;     Show all 13 Subjects
Abstract:
... We investigated the early innate immune responses induced in human intestinal epithelial cells (IEC) by the three defined Toxoplasma gondii genotype strains. Transcriptome analysis revealed that among differentially expressed genes, β-defensins distinguished the most IEC infected by fast- or slow-replicating T. gondii genotypes. Although β-defensin 1 and 3 genes were not expressed in host cells at ...
DOI:
10.1128/IAI.01086-10
PubMed:
21383053
PubMed Central:
PMC3088155

46. Molecular Analysis of Erythrocyte Invasion in Plasmodium falciparum Isolates from Senegal

Author:
Jennings, Cameron V.; Ahouidi, Ambroise D.; Zilversmit, Martine; Bei, Amy K.; Rayner, Julian; Sarr, Ousmane; Ndir, Omar; Wirth, Dyann F.; Mboup, Souleymane; Duraisingh, Manoj T.
Source:
Infection and immunity 2007 v.75 no.7 pp. 3531-3538
ISSN:
0019-9567
Subject:
parasites,   etc   ; Plasmodium falciparum; erythrocytes; gene amplification; genes; humans; immune system; malaria; protein synthesis; receptors; Senegal;     Show all 11 Subjects
Abstract:
... The human malaria parasite, Plasmodium falciparum, utilizes multiple ligand-receptor interactions for the invasion of human erythrocytes. Members of the reticulocyte binding protein homolog (PfRh) family have been shown to be critical for directing parasites to alternative erythrocyte receptors that define invasion pathways. Recent studies have identified gene amplification, sequence polymorphism, ...
DOI:
10.1128/IAI.00122-07
PubMed:
17470537
PubMed Central:
PMC1932937

47. Molecular basis for variable expression of merozoite surface antigen gp45 among American isolates of Babesia bigemina

Author:
Fisher, T.G.; McElwain, T.F.; Palmer, G.H.
Source:
Infection and immunity 2001 v.69 no.6 pp. 3782-3790
ISSN:
0019-9567
Subject:
parasites,   etc   ; B-lymphocytes; Babesia bigemina; Southern blotting; complementary DNA; disease outbreaks; epitopes; genes; genetic variation; genomics; glycoproteins; immunization; loci; merozoites; molecular cloning; molecular weight; monoclonal antibodies; oligodeoxyribonucleotides; open reading frames; polymerase chain reaction; sequence analysis; surface antigens; transcription (genetics); Mexico; Puerto Rico; South America;     Show all 26 Subjects
Abstract:
... Immunization with the merozoite surface glycoprotein gp45 induces protection against challenge using the homologous Babesia bigemina strain. However, gp45 B-cell epitopes are highly polymorphic among B. bigemina strains isolated from different geographical locations within North and South America. The molecular basis for this polymorphism was investigated using the JG-29 biological clone of a Mexi ...
DOI:
10.1128/IAI.69.6.3782-3790.2001
PubMed:
11349043
PubMed Central:
PMC98392

48. Molecular Cloning of a Trypanosoma cruzi Cell Surface Casein Kinase II Substrate, Tc-1, Involved in Cellular Infection

Author:
Augustine, Swinburne A.J.; Kleshchenko, Yuliya Y.; Nde, Pius N.; Pratap, Siddharth; Ager, Edward A.; Burns, James M. Jr.; Lima, Maria F.; Villalta, Fernando
Source:
Infection and immunity 2006 v.74 no.7 pp. 3922-3929
ISSN:
0019-9567
Subject:
parasite load; parasites,   etc   ; Chagas disease; Northern blotting; Southern blotting; Trypanosoma cruzi; amastigotes; amino acid sequences; antibodies; epimastigotes; fluorescent antibody technique; genes; heparin; humans; immunoglobulin G; molecular cloning; myoblasts; open reading frames; phosphorylation; precipitin tests; serine; solubilization; surface proteins; translation (genetics); trypomastigotes;     Show all 25 Subjects
Abstract:
... In this work, we report the cloning and characterization of the first cell surface casein kinase II (CKII) substrate (Tc-1) of Trypanosoma cruzi, the causative agent of Chagas' disease. Analysis of the gene sequence revealed a 1,653-bp open reading frame coding for 550 amino acid residues. Northern blot analysis showed a 4.5-kb transcript that is expressed in invasive trypomastigotes but not in no ...
DOI:
10.1128/IAI.00045-06
PubMed:
16790765
PubMed Central:
PMC1489715

49. Molecular Factors and Biochemical Pathways Induced by Febrile Temperature in Intraerythrocytic Plasmodium falciparum Parasites

Author:
Oakley, Miranda S.M.; Kumar, Sanjai; Anantharaman, Vivek; Zheng, Hong; Mahajan, Babita; Haynes, J. David; Moch, J. Kathleen; Fairhurst, Rick; McCutchan, Thomas F.; Aravind, L.
Source:
Infection and immunity 2007 v.75 no.4 pp. 2012-2025
ISSN:
0019-9567
Subject:
parasites,   etc   ; Plasmodium falciparum; bioassays; biochemical pathways; bioinformatics; cell death; cell membranes; cytoplasm; death; erythrocytes; gene expression regulation; genes; heat treatment; malaria; microarray technology; prediction; proteins; temperature; transcription (genetics); vaccines; virulence;     Show all 21 Subjects
Abstract:
... Intermittent episodes of febrile illness are the most benign and recognized symptom of infection with malaria parasites, although the effects on parasite survival and virulence remain unclear. In this study, we identified the molecular factors altered in response to febrile temperature by measuring differential expression levels of individual genes using high-density oligonucleotide microarray tec ...
DOI:
10.1128/IAI.01236-06
PubMed:
17283083
PubMed Central:
PMC1865691

50. MyD88-Deficient Mice Exhibit Decreased Parasite-Induced Immune Responses but Reduced Disease Severity in a Murine Model of Neurocysticercosis

Author:
Mishra, Bibhuti B.; Gundra, Uma Mahesh; Wong, Kondi; Teale, Judy M.
Source:
Infection and immunity 2009 v.77 no.12 pp. 5369-5379
ISSN:
0019-9567
Subject:
parasites,   etc   ; Mesocestoides corti; T-lymphocytes; animal models; blood-brain barrier; central nervous system; death; disease severity; fibrinogen; genes; humans; immune response; inflammation; interferon-gamma; interleukin-6; mice; neurocysticercosis; neuropathology; permeability; tumor necrosis factor-alpha;     Show all 20 Subjects
Abstract:
... The symptomatic phase of neurocysticercosis (NCC), a parasitic disease of the central nervous system (CNS) in humans, is characterized by inflammatory responses leading to neuropathology and, in some cases, death. In an animal model of NCC in which mice were intracranially inoculated with the parasite Mesocestoides corti, the infection in mice lacking the myeloid differentiation primary response g ...
DOI:
10.1128/IAI.00455-09
PubMed:
19786565
PubMed Central:
PMC2786461