Jump to Main Content
- Yang, Guang, et al. Show all 11 Authors
- Insect biochemistry and molecular biology 2017 v.89 pp. 71-78
- DNA-directed RNA polymerase; Plutella xylostella; TATA box; gene editing; genes; loci; messenger RNA; mutagenesis; pests; phenotype; plasmids; small interfering RNA; small nuclear RNA
- ... RNA polymerase type III (Pol-III) promoters such as U6 are commonly used to express small RNAs, including short hairpin RNAs (shRNAs) and single guide RNAs (sgRNAs). Functional U6 promoters are widely used in CRISPR systems, and their characterization can facilitate genome editing of non-model organisms. In the present study, six U6 small nuclear RNA (snRNA) promoters containing two conserved elem ...