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- Thairu, M. W.; Skidmore, I. H.; Bansal, R.; Nováková, E.; Hansen, T. E.; Li‐Byarlay, H.; Wickline, S. A.; Hansen, A. K.
- Insect molecular biology 2017 v.26 no.3 pp. 356-368
- Acyrthosiphon pisum; Aphis glycines; RNA interference; Schizaphis graminum; amino acids; carotenes; emulsions; genes; insects; metabolism; methodology; nanoparticles; phenotype; pigmentation; small interfering RNA
- ... RNA interference (RNAi) has emerged as a promising method for validating gene function; however, its utility in nonmodel insects has proven problematic, with delivery methods being one of the main obstacles. This study investigates a novel method of RNAi delivery in aphids, the aerosolization of short interfering RNA (siRNA)–nanoparticle complexes. By using nanoparticles as a siRNA carrier, the li ...
- Xu, L.; Huang, H.‐J.; Zhou, X.; Liu, C.‐W.; Bao, Y.‐Y.
- Insect molecular biology 2017 v.26 no.3 pp. 277-285
- Nilaparvata lugens; RNA interference; adults; diet; eggs; females; galactolipids; genes; hatching; humans; hydrolysis; insects; messenger RNA; oocytes; pancreas; plant pests; rice; seedlings; transcriptome
- ... In this study, we identified a pancreatic lipase‐related protein 2 (PLRP2) gene through searching the transcriptome data of the brown planthopper, Nilaparvata lugens, a monophagous rice pest. PLRP2 mRNAs were first isolated in the human pancreas and play an important role in hydrolysis of galactolipids from the diet. Although homologous PLRP2 genes have been identified in many insect species, thei ...