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1. Alginate-whey an effective and green adsorbent for crystal violet removal: Kinetic, thermodynamic and mechanism studies

Author:
Djelad, Amal; Mokhtar, Adel; Khelifa, Amine; Bengueddach, Abdelkader; Sassi, Mohamed
Source:
International journal of biological macromolecules 2019 v.139 pp. 944-954
ISSN:
0141-8130
Subject:
Fourier transform infrared spectroscopy; Gibbs free energy; absorption; adsorbents; adsorption; aqueous solutions; bacteria; biocomposites; enthalpy; entropy; equations; gelation; gentian violet; pH; scanning electron microscopy; sodium alginate; temperature; whey
Abstract:
... In this study, a novel biocomposite beads is developed by gelling sodium alginate (ALG) solutions in acid whey (Aw). The formation of alginate-whey biocomposite (ALG-Aw) was confirmed by FTIR analysis where the corresponding spectrum showed the presence of characteristic absorption bands of both ALG and Aw. SEM analysis showed the presence of lactic bacteria immobilized in the alginate matrix lead ...
DOI:
10.1016/j.ijbiomac.2019.08.068

2. A lytic polysaccharide monooxygenase from Myceliophthora thermophila and its synergism with cellobiohydrolases in cellulose hydrolysis

Author:
Zhou, Haichuan; Li, Tang; Yu, Zuochen; Ju, Jiu; Zhang, Huiyan; Tan, Haidong; Li, Kuikui; Yin, Heng
Source:
International journal of biological macromolecules 2019 v.139 pp. 570-576
ISSN:
0141-8130
Subject:
Myceliophthora thermophila; biorefining; cellulose; cellulose 1,4-beta-cellobiosidase; endo-1,4-beta-glucanase; enzyme activity; hydrolysis; models; polymers
Abstract:
... Lytic polysaccharide monooxygenases (LPMOs) have attracted vast attention because of their unique mechanism of oxidative degradation of carbohydrate polymers and the potential application in biorefineries. This study characterized a novel LPMO from Myceliophthora thermophila, denoted MtLPMO9L. The structure model of the enzyme indicated that it belongs to the C1-oxidizing LPMO, which has neither a ...
DOI:
10.1016/j.ijbiomac.2019.08.004

3. A novel nucleic acid based bio-assay toward recognition of Haemophilus influenza using bioconjugation and DNA hybridization method

Author:
Mobed, Ahmad; Nami, Fatemeh; Hasanzadeh, Mohammad; Hassanpour, Soodabeh; Saadati, Arezoo; Mokhtarzadeh, Ahad
Source:
International journal of biological macromolecules 2019 v.139 pp. 1239-1251
ISSN:
0141-8130
Subject:
Haemophilus; base pair mismatch; beta-lactamase; chemical bonding; complementary DNA; electrodes; gold; influenza; monitoring; nucleic acid hybridization; nucleotide sequences; particle size; penicillins; proteins; scanning electron microscopes; scanning electron microscopy; toluidine blue
Abstract:
... Haemophilus influenza (H. influenza) is a gram negative coccobacillus pathogenic microorganism. H. influenza produces beta-lactamases, and it is also able to modify its penicillin-binding proteins, so it has gained resistance to the penicillin family of antibiotics. In this work, a novel sensitive approach was established for the monitoring of H. influenza using DNA based bio-assay. For the first ...
DOI:
10.1016/j.ijbiomac.2019.08.036

4. A novel starch: Characterizations of starches separated from tea (Camellia sinensis (L.) O. Ktze) seed

Author:
Huang, Jiajia; Yuan, Ming'an; Kong, Xiangli; Wu, Dianxing; Zheng, Zhaisheng; Shu, Xiaoli
Source:
International journal of biological macromolecules 2019 v.139 pp. 1085-1091
ISSN:
0141-8130
Subject:
Camellia sinensis; amylopectin; amylose; gelatinization temperature; gels; polymerization; seeds; starch granules; tea; viscosity; water solubility
Abstract:
... The physicochemical, thermal and crystal properties of starches isolated from 3 different tea (Camellia sinensis (L.) O. Ktze) seeds were analyzed in this study. The shape of tea starch granules were flat spherical or oval shape, showed unimodal or bimodal distribution with average size of around 9 μm. Tea starch was typical A-type starch. Apparent amylose contents of three tea seed starches range ...
DOI:
10.1016/j.ijbiomac.2019.08.044

5. Anti-ageing and antioxidant effects of sulfate oligosaccharides from green algae Ulva lactuca and Enteromorpha prolifera in SAMP8 mice

Author:
Liu, Xiao-yan; Liu, Dan; Lin, Guo-peng; Wu, Yi-jing; Gao, Lu-ying; Ai, Chao; Huang, Yi-fan; Wang, Ming-fu; El-Seedi, Hesham R.; Chen, Xin-hua; Zhao, Chao
Source:
International journal of biological macromolecules 2019 v.139 pp. 342-351
ISSN:
0141-8130
Subject:
Coriobacteriaceae; Desulfovibrio; Fourier transform infrared spectroscopy; Planococcaceae; Ulva lactuca; Ulva prolifera; Verrucomicrobiaceae; advanced glycation end products; agar gel electrophoresis; antioxidant activity; antioxidants; catalase; drugs; functional foods; gas chromatography-mass spectrometry; gene expression regulation; genes; glutathione; high-throughput nucleotide sequencing; interferon-gamma; interleukin-6; malondialdehyde; mice; neurons; oligosaccharides; ribosomal RNA; sulfates; superoxide dismutase; telomerase; therapeutics; tumor necrosis factor-alpha
Abstract:
... Oligosaccharides from green algae Ulva lactuca (ULO) and Enteromorpha prolifera (EPO) were used for investigation of anti-ageing effects and the underlying mechanism in SAMP8 mice. The structural properties of ULO and EPO were analyzed by fourier-transform infrared spectroscopy, gas chromatography–mass spectrometry, and agarose gel electrophoresis. These oligosaccharides enhanced the glutathione, ...
DOI:
10.1016/j.ijbiomac.2019.07.195

6. A polysaccharide from Huaier ameliorates cisplatin nephrotoxicity by decreasing oxidative stress and apoptosis via PI3K/AKT signaling

Author:
Fang, Liang; Zhang, Yongzhen; Wang, Qi; Zang, Yuanwei; Li, Zeyan; Duan, Zhichen; Ren, Juchao; Xu, Zhonghua
Source:
International journal of biological macromolecules 2019 v.139 pp. 932-943
ISSN:
0141-8130
Subject:
Trametes; adverse effects; apoptosis; cell cycle checkpoints; cisplatin; drug therapy; immunomodulators; inflammation; kidneys; mitochondria; molecular weight; neoplasms; nephrotoxicity; oxidative stress; phosphatidylinositol 3-kinase; polysaccharides; renal function; signal transduction
Abstract:
... Cisplatin (CP), a common chemotherapy drug used in treatment of malignant tumors. Due to various side effects such as nephrotoxicity (kidney damage), it's efficiency and therapeutic application are limited. This study focuses on finding a suitable drug that would attenuate the side effects like kidney damage, caused by CP. Huaier polysaccharide (HP-1), an extraction of Trametes robiniophila Murr, ...
DOI:
10.1016/j.ijbiomac.2019.07.219

7. Application of coatings formed by chitosan and Mentha essential oils to control anthracnose caused by Colletotrichum gloesporioides and C. brevisporum in papaya (Carica papaya L.) fruit

Author:
dos Passos Braga, Selma; Lundgren, Giovanna Alencar; Macedo, Samara Alves; Tavares, Josean Fechine; dos Santos Vieira, Willie Anderson; Câmara, Marcos Paz Saraiva; de Souza, Evandro Leite
Source:
International journal of biological macromolecules 2019 v.139 pp. 631-639
ISSN:
0141-8130
Subject:
Carica papaya; Colletotrichum; Glomerella cingulata; Mentha piperita; anthracnose; chitosan; coatings; culture media; essential oils; fruits; fungicides; papayas; synergism
Abstract:
... This study investigated the efficacy of coatings formed by chitosan (Chi) and Mentha piperita L. (MPEO) or M. × villosa Huds (MVEO) essential oil to control the development of antrachnnose in papaya fruit caused by Colletotrichum gloeosporioides and C. brevisporum. Chi (2.5–10 mg/mL), MPEO and MVEO (0.15–1.25 μL/mL) alone effectively inhibited the growth of C. gloeosporioides and C. brevisporum is ...
DOI:
10.1016/j.ijbiomac.2019.08.010

8. Aspartic protease from Aspergillus niger: Molecular characterization and interaction with pepstatin A

Author:
Purushothaman, Kavya; Bhat, Sagar Krishna; Singh, Sridevi Annapurna; Marathe, Gopal Kedihithlu; Appu Rao, Appu Rao G.
Source:
International journal of biological macromolecules 2019 v.139 pp. 199-212
ISSN:
0141-8130
Subject:
Aspergillus niger; aspergillopepsin; catalytic activity; chromatography; dried skim milk; feed industry; fluorescence; functional properties; fungi; gelatin; genetic engineering; gluten; hemoglobin; hydrolysis; models; molecular weight; pH; polypeptides; proteolysis; solid state fermentation; soy flour; temperature; thermal stability; trypsin
Abstract:
... In the pursuit of industrial aspartic proteases, aspergillopepsin A-like endopeptidase from the fungi Aspergillus niger, was identified and cultured by solid state fermentation. Conventional chromatographic techniques were employed to purify the extracellular aspartic protease to apparent homogeneity. The enzyme was found to have single polypeptide chain with a molecular mass of 50 ± 0.5 kDa. The ...
DOI:
10.1016/j.ijbiomac.2019.07.133

9. Assessing virus like particles formation and r-HBsAg aggregation during large scale production of recombinant hepatitis B surface antigen from Pichia pastoris

Author:
Hosseini, Seyed Nezamedin; Sarvari, Taravat; Bashiri, Ghazal; Khatami, Maryam; Shojaosadati, Seyed Abbas
Source:
International journal of biological macromolecules 2019 v.139 pp. 697-711
ISSN:
0141-8130
Subject:
Komagataella pastoris; acid deposition; heat treatment; hepatitis B; hepatitis B antigens; high performance liquid chromatography; immunoaffinity chromatography; ion exchange chromatography; light scattering; recombinant proteins; transmission electron microscopy; ultrafiltration; vaccines; viruses; Iran
Abstract:
... The aggregation of recombinant proteins in the different stages of purification leads to the loss of a considerable portion of target protein and reduction in the process efficiency. As the active HBsAg used in Hepatitis B vaccine production is in the form of virus-like particle (VLP), therefore the time and stages at which the VLP assembling happened through the process would be important. The ai ...
DOI:
10.1016/j.ijbiomac.2019.08.019

10. Binding interactions of a series of sulfonated water-soluble resorcinarenes with bovine liver catalase

Author:
Collazos, Nicole; García, Germán; Malagón, Andrés; Caicedo, Obradith; Vargas, Edgar F.
Source:
International journal of biological macromolecules 2019 v.139 pp. 75-84
ISSN:
0141-8130
Subject:
active sites; catalase; cattle; fluorescence; human health; liver; moieties; proteins; ultraviolet-visible spectroscopy; water solubility
Abstract:
... Resorcinarenes are macrocyclic molecules that can bind different molecules in a supramolecular fashion. There are some sulfonated water-soluble derivatives that have been investigated to bind proteins avoiding fibrillation. The interaction with enzymes such as catalase (CAT) allows the interpretation of the possible effects of the use of resorcinarenes on human health or environmental applications ...
DOI:
10.1016/j.ijbiomac.2019.07.197

11. Biodegradable blends of graphene quantum dots and thermoplastic starch with solid-state photoluminescent and conductive properties

Author:
Chen, Jie; Long, Zhu; Wang, Shuangfei; Meng, Yahui; Zhang, Guoliang; Nie, Shuangxi
Source:
International journal of biological macromolecules 2019 v.139 pp. 367-376
ISSN:
0141-8130
Subject:
Fourier transform infrared spectroscopy; X-ray diffraction; biodegradability; dispersions; fluorescence; graphene; hydrogen bonding; mechanical testing; optical properties; packaging materials; photoluminescence; pressing; quantum dots; scanning electron microscopy; starch; thermoplastics; transmission electron microscopy; ultraviolet-visible spectroscopy
Abstract:
... Polymer composites based on blends of graphene quantum dots (GQDs) with thermoplastic starch (TPS) were prepared by melt-extrusion combined with hot pressing. The GQDs/TPS films were characterized as potential novel, high-performance, and ecofriendly composites replacing traditional non-biodegradable plastic packaging materials. GQDs stock solutions of different concentrations were incorporated in ...
DOI:
10.1016/j.ijbiomac.2019.07.211

12. Biodegradable protein films from gallic acid and the cataractous eye protein isolate

Author:
Parveen, Sultana; Chaudhury, Prasun; Dasmahapatra, Upala; Dasgupta, Swagata
Source:
International journal of biological macromolecules 2019 v.139 pp. 12-20
ISSN:
0141-8130
Subject:
2,2-diphenyl-1-picrylhydrazyl; Fourier transform infrared spectroscopy; X-ray diffraction; antioxidant activity; antioxidants; biodegradability; canola; casein; cataract; crosslinking; crystal structure; drugs; emulsions; eyes; gallic acid; polyacrylamide gel electrophoresis; protein isolates; scanning electron microscopy; surgery; tensile strength; thermal properties; thermogravimetry; trypsin
Abstract:
... Films have been prepared from the natural antioxidant gallic acid and a proteinaceous source, the discarded emulsion obtained after cataract surgery referred to as the cataractous eye protein isolate (CEPI). Fourier transform spectroscopy (FTIR) and SDS PAGE studies confirmed the crosslinking of gallic acid with CEPI. The cross-section and surface of films were further characterized by scanning el ...
DOI:
10.1016/j.ijbiomac.2019.07.143

13. Biofunctionalized cellulose paper matrix for cell delivery applications

Author:
Agarwal, Tarun; Maiti, Tapas Kumar; Behera, Birendra; Ghosh, Sudip Kumar; Apoorva, Anupam; Padmavati, Manchikanti
Source:
International journal of biological macromolecules 2019 v.139 pp. 114-127
ISSN:
0141-8130
Subject:
adhesion; animal models; cellulose; derivatization; extracellular matrix; goats; liver; mice; paper; stem cells; tissue repair; toxicity
Abstract:
... The present study delineates the preparation, characterization, and application of (3-Aminopropyl)triethoxysilane (APTES)/Caprine liver-derived extracellular matrix (CLECM) coated paper matrix for cell delivery. Here, we exploited positively charged surface of the paper matrix (as imparted by APTES derivatization) to improve the biological responses of the cells. Our results demonstrated that the ...
DOI:
10.1016/j.ijbiomac.2019.07.201

14. Biomimetic hybrid scaffold consisting of co-electrospun collagen and PLLCL for 3D cell culture

Author:
Türker, Esra; Yildiz, Ümit Hakan; Arslan Yildiz, Ahu
Source:
International journal of biological macromolecules 2019 v.139 pp. 1054-1062
ISSN:
0141-8130
Subject:
adsorption; aqueous solutions; biodegradation; biomimetics; cell adhesion; cell culture; cell proliferation; cell viability; collagen; extracellular matrix; fibroblasts; lactones; mice; polyvinylpyrrolidone; solvents; tissue engineering; toxicity
Abstract:
... Electrospun collagen is commonly used as a scaffold in tissue engineering applications since it mimics the content and morphology of native extracellular matrix (ECM) well. This report describes “toxic solvent free” fabrication of electrospun hybrid scaffold consisting of Collagen (Col) and Poly(l-lactide-co-ε-caprolactone) (PLLCL) for three-dimensional (3D) cell culture. Biomimetic hybrid scaffol ...
DOI:
10.1016/j.ijbiomac.2019.08.082

15. Biophysical and biochemical characterization of a thermostable archaeal cyclophilin from Methanobrevibacter ruminantium

Author:
Kaushik, Vineeta; Prasad, Saumya; Goel, Manisha
Source:
International journal of biological macromolecules 2019 v.139 pp. 139-152
ISSN:
0141-8130
Subject:
Escherichia coli; Methanobrevibacter; cell viability; cis-trans isomerism; cyclophilins; enzyme activity; eukaryotic cells; genes; heterologous gene expression; peptidylprolyl isomerase; protein folding; proteins; thermal stability
Abstract:
... The archaeal protein folding machinery is quite similar to that found in eukaryotes, especially in terms of shared components like chaperones. Cyclophilins are chaperones found in both eukaryotes and archaea, which catalyze the reversible cis-trans isomerization around peptidyl-prolyl imide bond (PPIase activity). Eukaryotes possess multiple cyclophilin genes, many of which have acquired divergent ...
DOI:
10.1016/j.ijbiomac.2019.07.149

16. Biopolymer-based biomaterials for accelerated diabetic wound healing: A critical review

Author:
Shah, Syed Ahmed; Sohail, Muhammad; Khan, Shahzeb; Minhas, Muhammad Usman; de Matas, Marcel; Sikstone, Victoria; Hussain, Zahid; Abbasi, Mudassir; Kousar, Mubeen
Source:
International journal of biological macromolecules 2019 v.139 pp. 975-993
ISSN:
0141-8130
Subject:
amputation; biocompatible materials; death; diabetes; drug delivery systems; drug development; enzymes; health care costs; pH; patients; polymers; tissue repair
Abstract:
... Non-healing, chronic wounds place a huge burden on healthcare systems as well as individual patients. These chronic wounds especially diabetic wounds will ultimately lead to compromised mobility, amputation of limbs and even death. Currently, wounds and limb ulcers associated with diabetes remain significant health issues; the associated healthcare cost ultimately leads to the increased clinical b ...
DOI:
10.1016/j.ijbiomac.2019.08.007

17. Carboxymethyl cellulose is a superior polyanion to dextran sulfate in stabilizing and enhancing the solubility of amorphous drug-polyelectrolyte nanoparticle complex

Author:
Dong, Bingxue; Hadinoto, Kunn
Source:
International journal of biological macromolecules 2019 v.139 pp. 500-508
ISSN:
0141-8130
Subject:
anions; carboxymethylcellulose; ciprofloxacin; crystallization; dextran sulfate; drugs; nanoparticles; pH; solubility; zeta potential
Abstract:
... Amorphous drug-polyanion nanoplex represents an effective solubility enhancement strategy of weakly-basic poorly-soluble drugs. While dextran sulfate (DXT) was chosen in most studies as the polyanion for nanoplex formation, drug-DXT nanoplex demonstrated poor long-term physical stability for drugs with high crystallization propensity, such as ciprofloxacin (CIP). Herein we hypothesized that amorph ...
DOI:
10.1016/j.ijbiomac.2019.08.023

18. Carboxymethyl cellulose/sodium alginate/chitosan biguanidine hydrochloride ternary system for edible coatings

Author:
Salama, Hend E.; Abdel Aziz, Mohamed S.; Alsehli, Mosa
Source:
International journal of biological macromolecules 2019 v.139 pp. 614-620
ISSN:
0141-8130
Subject:
Fourier transform infrared spectroscopy; X-ray diffraction; antibacterial properties; biodegradability; carboxymethylcellulose; chitosan; crosslinking; edible coatings; food preservation; fruits; industry; mechanical properties; permeability; polymers; shelf life; sodium alginate; spoilage; thermal properties; tomatoes; water solubility; water vapor; weight loss; wide-angle X-ray scattering
Abstract:
... Chitosan biguanidine hydrochloride (CBg) was prepared as a novel multifunctional biodegradable polymer with antimicrobial and excellent water-solubility properties, and used as a crosslinker to prepare carboxymethyl cellulose (CMC)/alginate (A)/CBg edible coating. FTIR confirmed the successful preparation of CBg and CMC/A/CBg films. Wide-angle X-ray diffraction showed that the amorphous structure ...
DOI:
10.1016/j.ijbiomac.2019.08.008

19. Cassava starch films reinforced with lignocellulose nanofibers from cassava bagasse

Author:
Travalini, Ana Paula; Lamsal, Buddhi; Magalhães, Washington Luiz Esteves; Demiate, Ivo Mottin
Source:
International journal of biological macromolecules 2019 v.139 pp. 1151-1161
ISSN:
0141-8130
Subject:
bagasse; cassava; cassava starch; coproducts; lignocellulose; mechanical properties; mechanical stress; nanoclays; nanofibers; nanoparticles; opacity; permeability; starch; thermal stability; water vapor
Abstract:
... Cassava bagasse, a high-fiber coproduct of cassava starch processing, was used to produce lignocellulose nanofibers (LCNF) to apply as reinforcement in cassava starch films. LCNF-reinforced cast starch films were evaluated for changes in structural, thermal and mechanical properties and compared with control films reinforced with commercial grade nanoclay (Nclay). Five different types of cassava s ...
DOI:
10.1016/j.ijbiomac.2019.08.115

20. Catalytic application of new manganese Schiff-base complex immobilized on chitosan-coated magnetic nanoparticles for one-pot synthesis of 3-iminoaryl-imidazo[1,2-a]pyridines

Author:
Rakhtshah, Jamshid; Yaghoobi, Fereshteh
Source:
International journal of biological macromolecules 2019 v.139 pp. 904-916
ISSN:
0141-8130
Subject:
Fourier transform infrared spectroscopy; X-ray diffraction; aldehydes; atomic absorption spectrometry; catalysts; catalytic activity; chemical bonding; chitosan; cyanides; energy-dispersive X-ray analysis; iron oxides; magnetism; manganese; nanoparticles; organosilicon compounds; pyridines; recycling; scanning electron microscopy; schiff bases; synthesis; triethylamine
Abstract:
... In this paper, new chitosan (CS) coated magnetic iron oxide nanoparticles (MNPs) supported manganese Schiff-base complex was designed, synthesized and screened as an efficient, reusable nanocatalyst by the three-component reaction of various aldehyde derivatives with trimethylsilyl cyanide and 2-aminopyridine for the synthesis of 3-iminoaryl-imidazo[1,2-a]pyridine (IAIP) derivatives. Fourier trans ...
DOI:
10.1016/j.ijbiomac.2019.08.054