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- Fan, Guangsen, et al. Show all 6 Authors
- International journal of biological macromolecules 2014 v.70 pp. 482-489
- thermal stability; xylooligosaccharides; xylan; open reading frames; xylanases; polyacrylamide gel electrophoresis; Aureobasidium pullulans; polymerization; amino acid sequences; thermophilic fungi; pH; temperature; molecular weight; Malbranchea; glycosides; introns; half life
- ... A thermostable xylanase (McXyn10) from the thermophilic fungus Malbranchea cinnamomea strain S168 was purified and biochemically characterized. The enzyme was purified to homogeneity with a molecular mass of 43.5kDa on SDS–PAGE. The optimal pH and temperature of the purified enzyme were pH 6.5 and 80°C, respectively. The enzyme showed a broad range of pH stability (pH 4.0–10.5), and was stable up ...
- Fan, Guangsen, et al. Show all 13 Authors
- International journal of biological macromolecules 2019 v.128 pp. 354-362
- Penicillium janthinellum; acid tolerance; agricultural wastes; catalytic activity; corn cobs; disulfide bonds; endo-1,4-beta-glucanase; enzymatic hydrolysis; industry; mutants; polymers; temperature; thermal stability; xylanases; xylose
- ... In order to increase the thermostability and catalytic efficiency of acidophilic GH11 xylanase, two disulfide bonds were introduced into crucial region of the enzyme. The xylanase PjxA, from Penicillium janthinellum MA21601, has attracted considerable attention due to its favorable acid-resistance; however, its poor thermostability and low enzymatic hydrolysis efficiency limit its application. In ...