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- Shi, Yaning, et al. Show all 5 Authors
- International journal of biological macromolecules 2020 v.155 pp. 403-410
- EDTA (chelating agent); Portunus trituberculatus; ammonium sulfate; anion exchange; bioinformatics; calcium; crabs; databases; enzyme activity; gel chromatography; hepatopancreas; hydrolysis; mass spectrometry; molecular weight; muscles; pH; proteins; serine proteinases; soybeans; temperature; transcriptome; trypsin inhibitors
- ... Serine proteases are thought to play a key role in the muscle softening of gazami crab (Portunus trituberculatus) during storage. A serine protease, Pt-sp2, was purified from the hepatopancreas of gazami crab using ammonium sulfate precipitation, anion-exchange and gel filtration chromatography, and was analyzed by mass spectrometry, transcriptome and bioinformatics. It revealed that Pt-sp2 was tr ...