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- Rehana, Kandhro; Jiang, Tao; Chen, Yanhua; Zhu, Juan; Tang, Shunming; Shen, Xingjia
- Journal of Asia-Pacific entomology 2019
- 3' untranslated regions; Bombyx mori; bioinformatics; gene expression regulation; genes; genetic vectors; larvae; luciferase; microRNA; plasmids; prediction; reverse transcriptase polymerase chain reaction; sericin; silk glands; tissues
- ... MiRNAs are small non-coding molecules, which can regulate a huge number of genes. Based on bioinformatics analysis, we found a target site in the 3′UTR of BmSer-1 for binding bmo-miR-3377-5p. By using semi-quantitative RT-PCR, we detected that miR-3377-5p and BmSer-1 were both more highly expressed in the middle silk gland than in other tissues of 3-day-old fifth-instar Bombyx mori larvae, implyin ...