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- Takemori, Nobuaki, et al. Show all 4 Authors
- Journal of Chromatography B 2014 v.967 pp. 36-40
- ambient temperature; chromatography; gels; ionization; mass spectrometry; peptides; phosphine; polyacrylamide; proteomics
- ... Enzymatic protein digestion in polyacrylamide gel has been used for sample pretreatment in mass spectrometry-based proteomics due to its effectiveness in removing contaminants that interfere with sample ionization. However, the difficulty of recovering the digested peptides from the solid gel matrix has been a drawback of this method. Here we have developed a novel in-gel digestion method to enhan ...