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- Sharma, Richa; Verma, Ved Vrat; Gupta, Rani
- Journal of Molecular Catalysis. B, Enzymatic 2013 v.91 pp. 8-16
- Pseudomonas aeruginosa; aminopeptidases; casein; catalytic activity; esters; feathers; fibrin; hemoglobin; keratin; models; pH; plasminogen; proteinases; serine; temperature
- ... A keratin degrading protease, Ker AP sharing peptide homology with putative aminopeptidase from Pseudomonas aeruginosa was cloned and expressed as an extracellular protein using pEZZ18-Escherichia coli HB101. It was a serine hydrolase with pH and temperature optima of pH 10 and 60°C. It had a t1/2 of 20.50min at 70°C. It hydrolyzed various complex proteins such as fibrin, hemoglobin, feather and c ...