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1. Prevalence of pathogens from Mollicutes class in cattle affected by respiratory diseases and molecular characteristics of Mycoplasma bovis field strains

Author:
Szacawa, Ewelina; Szymańska-Czerwińska, Monika; Niemczuk, Krzysztof; Dudek, Katarzyna; Woźniakowski, Grzegorz; Bednarek, Dariusz
Source:
Journal of Veterinary Research 2016 v.60 no.4 pp. 391-397
ISSN:
2450-8608
Subject:
DNA; Mycoplasma bovirhinis; Mycoplasma bovis; Ureaplasma diversum; bacteria; cattle; denaturing gradient gel electrophoresis; genes; herds; mutation; nose; pathogens; phylogeny; pneumonia; random amplified polymorphic DNA technique; sequence analysis
Abstract:
... Introduction: Mycoplasma bovis is one of the main pathogens involved in cattle pneumonia. Other mycoplasmas have also been directly implicated in respiratory diseases in cattle. The prevalence of different Mycoplasma spp. in cattle affected by respiratory diseases and molecular characteristics of M. bovis field strains were evaluated. Material and Methods: In total, 713 nasal swabs from 73 cattle ...
DOI:
10.1515/jvetres-2016-00058

2. Real-time quantitative PCR for detection and identification of Actinobacillus pleuropneumoniae serotype 2

Author:
Dors, Arkadiusz; Kowalczyk, Andrzej; Pomorska-Mól, Małgorzata
Source:
Journal of Veterinary Research 2016 v.60 no.3 pp. 253-256
ISSN:
2450-8608
Subject:
Actinobacillus pleuropneumoniae 2; agar; bacteria; biosynthesis; detection limit; financial economics; genes; herds; lungs; nose; pleuropneumonia; quantitative polymerase chain reaction; serotypes; swine; tonsils
Abstract:
... Introduction: Porcine pleuropneumonia inflicts important economic losses on most commercial herds. Detection of subclinical or chronic infection in animals still remains a challenge, as isolation and identification of A. pleuropneumoniae serotypes is difficult and quantification of the bacteria on agar plates is often almost impossible. The aim of the study was to develop and evaluate a serotype-s ...
DOI:
10.1515/jvetres-2016-0038