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You searched for: Journal Journal of agricultural and food chemistry Remove constraint Journal: Journal of agricultural and food chemistry Publication Year 2018 Remove constraint Publication Year: 2018 Source 2018 v.66 no.36 Remove constraint Source: 2018 v.66 no.36 Subject Escherichia coli Remove constraint Subject: Escherichia coli Subject glycosides Remove constraint Subject: glycosides Subject Sphingomonas Remove constraint Subject: Sphingomonas
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1. Glycoside Hydrolase Family 39 β-Xylosidase of Sphingomonas Showing Salt/Ethanol/Trypsin Tolerance, Low-pH/Low-Temperature Activity, and Transxylosylation Activity

Author:
Li, Na; Han, Xiaowei; Xu, Shujing; Li, Chunyan; Wei, Xin; Liu, Yu; Zhang, Rui; Tang, Xianghua; Zhou, Junpei; Huang, Zunxi
Source:
Journal of agricultural and food chemistry 2018 v.66 no.36 pp. 9465-9472
ISSN:
1520-5118
Subject:
Escherichia coli; Sphingomonas; amino acids; enzyme activity; ethanol; glycosides; microorganisms; moieties; pH; sodium chloride; sugars; trypsin; xylan 1,4-beta-xylosidase
Abstract:
... Mining for novel enzymes from new microorganisms is a way to obtain β-xylosidases with promising applications. A Sphingomonas β-xylosidase was expressed in Escherichia coli. The purified recombinant enzyme (rJB13GH39) was most active at pH 4.5 and 50 °C, retaining 10%–50% of its maximum activity at 0–20 °C. Most salts and chemical reagents including 3.0%–20.0% (w/v) NaCl showed little or no effect ...
DOI:
10.1021/acs.jafc.8b03327