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1. Soybean Glycinin- and β-Conglycinin-Induced Intestinal Damage in Piglets via the p38/JNK/NF-κB Signaling Pathway

Author:
Peng, Chenglu; Cao, Chengming; He, Mengchu; Shu, Yingshuang; Tang, Xuebing; Wang, Yuanhong; Zhang, Yu; Xia, Xiaodong; Li, Yu; Wu, Jinjie
Source:
Journal of agricultural and food chemistry 2018 v.66 no.36 pp. 9534-9541
ISSN:
1520-5118
Subject:
Duroc; animal models; antigens; beta-conglycinin; blood serum; caspase-3; glycinin; hypersensitivity; intestines; landraces; messenger RNA; mitogen-activated protein kinase; nitric oxide; phosphorylation; piglets; respiratory system; signal transduction; soybeans; suckling; transcription factor NF-kappa B; tumor necrosis factor-alpha
Abstract:
... β-Conglycinin (7S) and glycinin (11S) are known to induce a variety of hypersensitivity reactions involving the skin, intestinal tract, and respiratory tract. The present study aimed to identify the mechanism underlying the development of allergy to soybean antigen proteins, using piglets as an animal model. Weaned “Duroc × Landrace × Yorkshire” piglets were fed a diet supplemented with 7S or 11S ...
DOI:
10.1021/acs.jafc.8b03641

2. Hydrophobic Ethylcellulose/Gelatin Nanofibers Containing Zinc Oxide Nanoparticles for Antimicrobial Packaging

Author:
Liu, Yuyu; Li, Yang; Deng, Lingli; Zou, Lin; Feng, Fengqin; Zhang, Hui
Source:
Journal of agricultural and food chemistry 2018 v.66 no.36 pp. 9498-9506
ISSN:
1520-5118
Subject:
Escherichia coli; Fourier transform infrared spectroscopy; Staphylococcus aureus; X-ray diffraction; X-ray photoelectron spectroscopy; antimicrobial packaging; antimicrobial properties; contact angle; energy-dispersive X-ray analysis; food packaging; gelatin; hydrophobicity; mechanical testing; nanofibers; nanoparticles; polymers; reactive oxygen species; scanning electron microscopy; ultraviolet radiation; zinc oxide
Abstract:
... The ethylcellulose/gelatin solutions containing various concentrations of zinc oxide (ZnO) nanoparticles were electrospun, and the resultant nanofibers were characterized by scanning electron microscopy, energy dispersive X-ray, X-ray photoelectron spectrometer, X-ray diffraction, Fourier transform infrared spectroscopy, mechanical testing, water contact angle, and water stability. Results indicat ...
DOI:
10.1021/acs.jafc.8b03267

3. BrNAC055, a Novel Transcriptional Activator, Regulates Leaf Senescence in Chinese Flowering Cabbage by Modulating Reactive Oxygen Species Production and Chlorophyll Degradation

Author:
Fan, Zhong-qi; Tan, Xiao-Li; Chen, Jian-wen; Liu, Zong-li; Kuang, Jian-fei; Lu, Wang-jin; Shan, Wei; Chen, Jian-ye
Source:
Journal of agricultural and food chemistry 2018 v.66 no.36 pp. 9399-9408
ISSN:
1520-5118
Subject:
cabbage; chlorophyll; flowering; gels; genes; leaves; luciferase; metabolism; reactive oxygen species; respiratory burst; tobacco; transactivators
Abstract:
... Both NAC transcription factors (TFs) and reactive oxygen species (ROS) are known to be involved in leaf senescence. However, how NAC TFs modulate ROS metabolism associated with leaf senescence remains largely uncharacterized, especially during leaf senescence of postharvest economically leafy vegetables such as Chinese flowering cabbage. Here, we found that expression levels of two genes BrRbohB a ...
DOI:
10.1021/acs.jafc.8b02309

4. Colorimetric Immunoassay for Rapid Detection of Vibrio parahemolyticus Based on Mn2+ Mediates the Assembly of Gold Nanoparticles

Author:
Fu, Kaiyue; Zheng, Yan; Li, Juan; Liu, Yushen; Pang, Bo; Song, Xiuling; Xu, Kun; Wang, Juan; Zhao, Chao
Source:
Journal of agricultural and food chemistry 2018 v.66 no.36 pp. 9516-9521
ISSN:
1520-5118
Subject:
Vibrio parahaemolyticus; antibodies; ascorbic acid; color; colorimetry; detection limit; food pathogens; foodborne illness; gastroenteritis; immunoassays; immunoglobulin G; ions; magnetism; manganese; manganese dioxide; nanogold; nanoparticles; oysters; rapid methods
Abstract:
... Vibrio parahemolyticus (V. parahemolyticus) is an important food-borne pathogen that causes food poisoning and acute gastroenteritis in humans. Herein, a novel colorimetric immunoassay was presented for rapid detection of V. parahemolyticus using gold nanoparticles (18.1 nm diameter) as chromogenic substrate, whose combination of a magnetic bead-based sandwich immunoassay and an optical sensing sy ...
DOI:
10.1021/acs.jafc.8b02494

5. Comparative Metabolic Responses and Adaptive Strategies of Tea Leaves (Camellia sinensis) to N2 and CO2 Anaerobic Treatment by a Nontargeted Metabolomics Approach

Author:
Chen, Qi; Zhang, Yamin; Tao, Minming; Li, Mengshuang; Wu, Yun; Qi, Qi; Yang, Hua; Wan, Xiaochun
Source:
Journal of agricultural and food chemistry 2018 v.66 no.36 pp. 9565-9572
ISSN:
1520-5118
Subject:
Camellia sinensis; amino acid metabolism; anaerobic treatment; biochemical pathways; carbon dioxide; cluster analysis; galactose; gamma-aminobutyric acid; glucose; heat; leaves; mass spectrometry; metabolites; metabolomics; multivariate analysis; phenylalanine; tea; ultra-performance liquid chromatography
Abstract:
... It is well-known that anaerobic treatment has been considered as a utility process to accumulate γ-aminobutyric acid (GABA) in tea leaves. In this article, the nonvolatile differential compounds in picked-tea leaves between filled-N₂ treatment and filled-CO₂ treatment were compared in metabolic profiles and dynamic changes via ultrahigh performance liquid chromatography linked to a hybrid quadrupo ...
DOI:
10.1021/acs.jafc.8b03067

6. Glycoside Hydrolase Family 39 β-Xylosidase of Sphingomonas Showing Salt/Ethanol/Trypsin Tolerance, Low-pH/Low-Temperature Activity, and Transxylosylation Activity

Author:
Li, Na; Han, Xiaowei; Xu, Shujing; Li, Chunyan; Wei, Xin; Liu, Yu; Zhang, Rui; Tang, Xianghua; Zhou, Junpei; Huang, Zunxi
Source:
Journal of agricultural and food chemistry 2018 v.66 no.36 pp. 9465-9472
ISSN:
1520-5118
Subject:
Escherichia coli; Sphingomonas; amino acids; enzyme activity; ethanol; glycosides; microorganisms; moieties; pH; sodium chloride; sugars; trypsin; xylan 1,4-beta-xylosidase
Abstract:
... Mining for novel enzymes from new microorganisms is a way to obtain β-xylosidases with promising applications. A Sphingomonas β-xylosidase was expressed in Escherichia coli. The purified recombinant enzyme (rJB13GH39) was most active at pH 4.5 and 50 °C, retaining 10%–50% of its maximum activity at 0–20 °C. Most salts and chemical reagents including 3.0%–20.0% (w/v) NaCl showed little or no effect ...
DOI:
10.1021/acs.jafc.8b03327

7. Relationship between the Structure of Ionic Liquid and Its Enrichment Ability To Trace Fungicides from an Environmental Water Sample

Author:
Yang, Jiale; Fan, Chen; Tang, Gang; Zhang, Wenbing; Dong, Hongqiang; Liang, You; Wang, Yanfei; Zou, Mingqiang; Cao, Yongsong
Source:
Journal of agricultural and food chemistry 2018 v.66 no.36 pp. 9418-9425
ISSN:
1520-5118
Subject:
boscalid; carbon; cyprodinil; ionic liquids; liquid-phase microextraction; pyrimethanil
Abstract:
... To elucidate the relationship between the structure of ionic liquid (IL) and its enrichment ability to trace pesticides from an environmental water sample, a series of imidazole-based ILs were synthesized to extract four fungicides (boscalid, cyprodinil, fluazinam, and pyrimethanil) through an in situ ionic liquid dispersive liquid–liquid microextraction method. The results showed that aromatic he ...
DOI:
10.1021/acs.jafc.8b03244

8. Carotenoid and Tocochromanol Profiles during Kernel Development Make Consumption of Biofortified “Fresh” Maize an Option to Improve Micronutrient Nutrition

Author:
Cabrera-Soto, Luisa; Pixley, Kevin V.; Rosales-Nolasco, Aldo; Galicia-Flores, Luis A.; Palacios-Rojas, Natalia
Source:
Journal of agricultural and food chemistry 2018 v.66 no.36 pp. 9391-9398
ISSN:
1520-5118
Subject:
filling period; Estimated Average Requirement; hybrids; lysine; malnutrition; zinc; biofortification; carotenes; corn; tryptophan; ingestion
Abstract:
... Biofortification is a strategy to reduce micronutrient malnutrition. The aim of this study was to investigate whether consumption of biofortified fresh maize can supply nutritionally meaningful amounts of provitamin A carotenoids (PVA), zinc, lysine, and tryptophan. The accumulation patterns for PVA and tocochromanol compounds in developing grain of 23 PVA hybrids was studied, and nutritionally me ...
DOI:
10.1021/acs.jafc.8b01886

9. Critical Review and Perspectives on Food-Derived Antihypertensive Peptides

Author:
Miralles, Beatriz; Amigo, Lourdes; Recio, Isidra
Source:
Journal of agricultural and food chemistry 2018 v.66 no.36 pp. 9384-9390
ISSN:
1520-5118
Subject:
antihypertensive effect; bioavailability; clinical trials; food composition; health policy; industry; mechanism of action; peptides; therapeutics
Abstract:
... Food-derived peptides with antihypertensive properties have received great interest during the past 30 years. There is solid evidence of the effect of various peptide sequences in clinical trials, but their use in preventive or therapeutic treatments is not extensive. There are certain issues, such as the bioavailability or the mechanism of action, that would need to be clarified to establish a di ...
DOI:
10.1021/acs.jafc.8b02603

10. Development of an Inducible Secretory Expression System in Bacillus licheniformis Based on an Engineered Xylose Operon

Author:
Li, Youran; Jin, Ke; Zhang, Liang; Ding, Zhongyang; Gu, Zhenghua; Shi, Guiyang
Source:
Journal of agricultural and food chemistry 2018 v.66 no.36 pp. 9456-9464
ISSN:
1520-5118
Subject:
Bacillus licheniformis; GRAS substances; alpha-amylase; catabolite repression; consensus sequence; genetically engineered microorganisms; glucan 1,4-alpha-maltohydrolase; industrial applications; mutation; nucleotides; operon; plasmids; protein engineering; xylose
Abstract:
... The xylose operon can be an efficient biological component for regulatory expression uses in Bacillus licheniformis. However, its characteristic susceptibility to carbon catabolite repression (CCR) makes its application inconvenient. In this study, plasmids harboring the wild-type operons from three Bacillus species were constructed and introduced into B. licheniformis. These plasmids ensured secr ...
DOI:
10.1021/acs.jafc.8b02857

11. In Vitro Digestibility of Nanoporous Wheat Starch Aerogels

Author:
Ubeyitogullari, Ali; Brahma, Sandrayee; Rose, Devin J.; Ciftci, Ozan N.
Source:
Journal of agricultural and food chemistry 2018 v.66 no.36 pp. 9490-9497
ISSN:
1520-5118
Subject:
aerogels; cooking; crosslinking; functional foods; gelatinization temperature; in vitro digestibility; ingredients; nanopores; phosphorus; resistant starch; sodium; wheat starch; xerogels
Abstract:
... This study reports the in vitro digestibility of starch aerogels for the first time. The relative crystallinities of the wheat starch aerogels (WSAs) produced at gelatinization temperatures of 120 °C (WSA-120C), 130 °C (WSA-130C), and 120 °C with the addition of sodium metaphosphate (STMP) (WSA-STMP) and xerogel were similar. However, WSA-120C showed the highest amylose–lipid complex content. The ...
DOI:
10.1021/acs.jafc.8b03231
CHORUS:
10.1021/acs.jafc.8b03231

12. Solid Lipid–Polymer Hybrid Nanoparticles by In Situ Conjugation for Oral Delivery of Astaxanthin

Author:
Wang, Taoran; Hu, Qiaobin; Lee, Ji-Young; Luo, Yangchao
Source:
Journal of agricultural and food chemistry 2018 v.66 no.36 pp. 9473-9480
ISSN:
1520-5118
Subject:
antioxidant activity; astaxanthin; bioactive compounds; bovine serum albumin; dextran; encapsulation; gastrointestinal system; lipids; lipophilicity; models; molecular weight; nanocarriers; nanoparticles; oxidation; particle size; polymers; temperature
Abstract:
... Solid lipid–polymer hybrid nanoparticles (SLPN) are nanocarriers made from a combination of polymers and lipids, integrating the advantages of biocompatible lipid-based nanoparticles and gastrointestinal (GI)-stable polymeric nanoparticles. In this study, a novel preparation strategy was proposed to fabricate GI-stable SLPN through in situ conjugation between oxidized dextran and bovine serum albu ...
DOI:
10.1021/acs.jafc.8b02827
CHORUS:
10.1021/acs.jafc.8b02827

13. Fabrication of Resveratrol-Loaded Whey Protein–Dextran Colloidal Complex for the Stabilization and Delivery of β-Carotene Emulsions

Author:
Yi, Jiang; Liu, Yuexiang; Zhang, Yuzhu; Gao, Luyu
Source:
Journal of agricultural and food chemistry 2018 v.66 no.36 pp. 9481-9489
ISSN:
1520-5118
Subject:
beta-carotene; colloids; emulsions; fluorescence emission spectroscopy; functional foods; gel electrophoresis; glycation; heat; heat treatment; hydrophobic bonding; hydrophobicity; ionic strength; nanoparticles; pH; protocols; resveratrol; storage quality; transmission electron microscopy; ultraviolet radiation; whey; whey protein isolate
Abstract:
... The effects of resveratrol (RES)-loaded whey protein isolate (WPI)–dextran nanocomplex on the physicochemical stability of β-carotene (BC) emulsions were evaluated. WPI–dextran was prepared by Maillard-based glycation and confirmed with gel electrophoresis and OPA assay. WPI–RES and WPI–dextran–RES nanoparticles were prepared with a simple nanocomplexation protocol. Fluorescence spectra indicated ...
DOI:
10.1021/acs.jafc.8b02973

14. Sulforaphane Rescues Ethanol-Suppressed Angiogenesis through Oxidative and Endoplasmic Reticulum Stress in Chick Embryos

Author:
Wang, Guang; Nie, Jia-hui; Bao, Yongping; Yang, Xuesong
Source:
Journal of agricultural and food chemistry 2018 v.66 no.36 pp. 9522-9533
ISSN:
1520-5118
Subject:
angiogenesis; antioxidants; apoptosis; bioactive compounds; chickens; chicks; chorioallantoic membrane; endoplasmic reticulum; endoplasmic reticulum stress; enzymes; ethanol; gene expression; models; sulforaphane; yolk sac
Abstract:
... Our previous study showed that ethanol exposure inhibited embryonic angiogenesis mainly due to the excessive stimulation of reactive oxygen species (ROS) production. In this study, we investigated whether sulforaphane (SFN), a known dietary bioactive compound, could ameliorate ethanol-suppressed angiogenesis using chick embryo angiogenesis models. Using chick yolk sac membrane (YSM) and chorioalla ...
DOI:
10.1021/acs.jafc.8b02949

15. Development of Nanoscale Oil Bodies for Targeted Treatment of Lung Cancer

Author:
Chiang, Chung-Jen; Lin, Li-Jen; Wu, Chia-Pei; Chen, Chih-Jung; Chao, Yun-Peng
Source:
Journal of agricultural and food chemistry 2018 v.66 no.36 pp. 9438-9445
ISSN:
1520-5118
Subject:
Escherichia coli; antineoplastic activity; antineoplastic agents; epidermal growth factor receptors; hydrophobicity; lipid bodies; lung neoplasms; neoplasm cells; oleosin; phospholipids; plant fats and oils; therapeutics
Abstract:
... Lung cancer is the most widespread disease and is frequently associated with a high level of epidermal growth factor receptor (EGFR). This study was thus conducted to provide a proof-of-concept approach for targeted therapy of lung cancer by development of nanoscale oil bodies (NOBs). This was carried out by fusion of anti-EGFR affibody (ZEGFR₂) with oleosin (Ole), a structure protein of plant see ...
DOI:
10.1021/acs.jafc.8b02972

16. Pesticide Detox by Design

Author:
Casida, John E.
Source:
Journal of agricultural and food chemistry 2018 v.66 no.36 pp. 9379-9383
ISSN:
1520-5118
Subject:
biochemical pathways; crops; environmental impact; fungicides; herbicides; human health; insecticides; pesticidal properties
Abstract:
... Detoxification (detox) plays a major role in pesticide action and resistance. The mechanisms involved are sometimes part of the discovery and development process in seeking new biochemical targets and metabolic pathways. Genetically modified and chemical-safener-modified crops are a marked exception and often involve herbicide detox by design to achieve the required crop tolerance. This perspectiv ...
DOI:
10.1021/acs.jafc.8b02449

17. Physico-Chemical Changes of Composite Whey Protein Hydrogels in Simulated Gastric Fluid Conditions

Author:
Ozel, Baris; Aydin, Ozlem; Grunin, Leonid; Oztop, Mecit H.
Source:
Journal of agricultural and food chemistry 2018 v.66 no.36 pp. 9542-9555
ISSN:
1520-5118
Subject:
Daucus carota; carrots; gastric juice; gum tragacanth; hardness; hydrogels; nuclear magnetic resonance spectroscopy; pectins; physicochemical properties; polymers; whey protein isolate; xanthan gum
Abstract:
... Polysaccharide blended whey protein isolate (WPI) hydrogels were developed for the delivery of black carrot (Daucus carota) concentrate as bioactive agent in simulated gastric fluid (SGF). Pectin (PC), gum tragacanth (GT), and xanthan gum (XG) were blended as additional polymers to modulate the release characteristics of the WPI hydrogels. Experiments showed that sole whey protein (C), XG, and GT ...
DOI:
10.1021/acs.jafc.8b02829

18. Potential of Fructooligosaccharides and Xylooligosaccharides as Substrates To Counteract the Undesirable Effects of Several Antibiotics on Elder Fecal Microbiota: A First in Vitro Approach

Author:
Míguez, Beatriz; Gómez, Belén; Parajó, Juan Carlos; Alonso, José L.
Source:
Journal of agricultural and food chemistry 2018 v.66 no.36 pp. 9426-9437
ISSN:
1520-5118
Subject:
Bacteroides; Bifidobacterium; Clostridia; Lactobacillus; acetates; antibiotics; biochemical pathways; butyrates; elderly; fermentation; fructooligosaccharides; intestinal microorganisms; xylooligosaccharides
Abstract:
... Fructooligosaccharides (FOS) and xylooligosaccharides (XOS) were employed as substrates for in vitro fermentations to assess their capacity to counteract the effects caused by three antibiotics (ABs) at different doses on the elderly gut microbiota and its metabolic activity. The AB type and dose scarcely affected the total bacterial numbers and the microbiota composition after 24 h. However, in t ...
DOI:
10.1021/acs.jafc.8b02940

19. Maillard-Reacted Whey Protein Isolates Enhance Thermal Stability of Anthocyanins over a Wide pH Range

Author:
Qin, Xinguang; Yuan, Dan; Wang, Qi; Hu, Zhongze; Wu, Yang; Cai, Jie; Huang, Qingrong; Li, Shuyi; Liu, Gang
Source:
Journal of agricultural and food chemistry 2018 v.66 no.36 pp. 9556-9564
ISSN:
1520-5118
Subject:
Maillard reaction products; anthocyanins; antioxidant activity; color; dispersions; fluorescence emission spectroscopy; functional foods; glucose; heat stability; heat treatment; hydrophobic bonding; industrial applications; ingredients; pH; particle size; whey protein isolate
Abstract:
... The poor thermal and acid stabilities of anthocyanins greatly limit their industrial applications as functional food ingredients. This work investigated the ability of the Maillard reaction products (MRPs) of whey protein isolates and glucose to enhance the thermal stability of anthocyanins over the pH range of 2.0–7.0. Anthocyanin dispersions were subjected to up to 120 min of thermal treatment a ...
DOI:
10.1021/acs.jafc.8b03125

20. Characterization of UDP-Glycosyltransferase Involved in Biosynthesis of Ginsenosides Rg1 and Rb1 and Identification of Critical Conserved Amino Acid Residues for Its Function

Author:
Lu, Jun; Yao, Lu; Li, Jin-Xin; Liu, Shu-Jie; Hu, Yan-Ying; Wang, Shi-Hui; Liang, Wen-Xia; Huang, Lu-Qi; Dai, Yu-Jie; Wang, Juan; Gao, Wen-Yuan
Source:
Journal of agricultural and food chemistry 2018 v.66 no.36 pp. 9446-9455
ISSN:
1520-5118
Subject:
Panax ginseng; Saccharomyces cerevisiae; active sites; amino acids; bioactive properties; biosynthesis; gene overexpression; genetically modified organisms; ginsenosides; glucose; models; moieties; molecular dynamics; mutational analysis; transferases
Abstract:
... Ginsenosides attract great attention for their bioactivities. However, their contents are low, and many UDP-glycosyltransferases (UGTs) that play crucial roles in the ginsenoside biosynthesis pathways have not been identified, which hinders the biosynthesis of ginsenosides. In this study, we reported that one UDP-glycosyltransferase, UGTPg71A29, from Panax ginseng could glycosylate C20–OH of Rh₁ a ...
DOI:
10.1021/acs.jafc.8b02544