PubAg

Main content area

You searched for: Journal Journal of agricultural and food chemistry Remove constraint Journal: Journal of agricultural and food chemistry Publication Year 2018 Remove constraint Publication Year: 2018 Subject Escherichia coli Remove constraint Subject: Escherichia coli Subject glycosides Remove constraint Subject: glycosides
Start Over

Search

3  Search Results

1 - 3 of 3
Number of results to display per page

Search Results

1. Glycoside Hydrolase Family 39 β-Xylosidase of Sphingomonas Showing Salt/Ethanol/Trypsin Tolerance, Low-pH/Low-Temperature Activity, and Transxylosylation Activity

Author:
Li, Na; Han, Xiaowei; Xu, Shujing; Li, Chunyan; Wei, Xin; Liu, Yu; Zhang, Rui; Tang, Xianghua; Zhou, Junpei; Huang, Zunxi
Source:
Journal of agricultural and food chemistry 2018 v.66 no.36 pp. 9465-9472
ISSN:
1520-5118
Subject:
Escherichia coli; Sphingomonas; amino acids; enzyme activity; ethanol; glycosides; microorganisms; moieties; pH; sodium chloride; sugars; trypsin; xylan 1,4-beta-xylosidase
Abstract:
... Mining for novel enzymes from new microorganisms is a way to obtain β-xylosidases with promising applications. A Sphingomonas β-xylosidase was expressed in Escherichia coli. The purified recombinant enzyme (rJB13GH39) was most active at pH 4.5 and 50 °C, retaining 10%–50% of its maximum activity at 0–20 °C. Most salts and chemical reagents including 3.0%–20.0% (w/v) NaCl showed little or no effect ...
DOI:
10.1021/acs.jafc.8b03327

2. Discovery and Characterization of a Novel Chitosanase from Paenibacillus dendritiformis by Phylogeny-Based Enzymatic Product Specificity Prediction

Author:
Sun, Huihui; Mao, Xiangzhao; Guo, Na; Zhao, Ling; Cao, Rong; Liu, Qi
Source:
Journal of agricultural and food chemistry 2018 v.66 no.18 pp. 4645-4651
ISSN:
1520-5118
Subject:
Escherichia coli; Paenibacillus; chitosan; chitosanase; electrospray ionization mass spectrometry; genes; genome mining; glycosides; molecular weight; pH; polyacrylamide gel electrophoresis; prediction; sequence alignment; thin layer chromatography
Abstract:
... In the process of genome mining for novel chitosanases by phylogeny-based enzymatic product specificity prediction, a gene named Csn-PD from Paenibacillus dendritiformis was discovered. The enzyme was classified as a member of the GH46 family of glycoside hydrolase based on sequence alignment, and it was functionally expressed in Escherichia coli BL21 (DE3). The recombinant chitosanase was purifie ...
DOI:
10.1021/acs.jafc.7b06067

3. Structural Insights into the Thermophilic Adaption Mechanism of Endo-1,4-β-Xylanase from Caldicellulosiruptor owensensis

Author:
Liu, Xin; Liu, Tengfei; Zhang, Yuebin; Xin, Fengjiao; Mi, Shuofu; Wen, Boting; Gu, Tianyi; Shi, Xinyuan; Wang, Fengzhong; Sun, Lichao
Source:
Journal of agricultural and food chemistry 2018 v.66 no.1 pp. 187-193
ISSN:
1520-5118
Subject:
Caldicellulosiruptor owensensis; Escherichia coli; cations; crystal structure; endo-1,4-beta-xylanase; genes; glycosides; half life; industrial applications; molecular dynamics; pH; substrate specificity; temperature; thermal stability; thermophilic microorganisms; xylan; xylooligosaccharides
Abstract:
... Xylanases (EC 3.2.1.8) are a kind of enzymes degrading xylan to xylooligosaccharides (XOS) and have been widely used in a variety of industrial applications. Among them, xylanases from thermophilic microorganisms have distinct advantages in industries that require high temperature conditions. The CoXynA gene, encoding a glycoside hydrolase (GH) family 10 xylanase, was identified from thermophilic ...
DOI:
10.1021/acs.jafc.7b03607