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- Author:
- Kaur, Lovedeep; Rutherfurd, Shane M.; Moughan, Paul J.; Drummond, Lynley; Boland, Mike J.
- Source:
- Journal of agricultural and food chemistry 2010 v.58 no.8 pp. 5068–5073
- ISSN:
- 0021-8561
- Subject:
- cysteine proteinases; in vitro digestion; proteins; kiwifruit; stomach; enzyme activity; dietary protein; pepsin; Actinidia deliciosa
- Abstract:
- ... Consumption of kiwifruit has long been claimed anecdotally to assist in gastric digestion. This has generally been assumed to be due to the presence of the proteolytic enzyme actinidin; however, there is little published evidence supporting this assumption. This paper reports the findings of an in vitro study that examined the effect of kiwifruit proteases (actinidin) on the digestion of a range o ...
- DOI:
- 10.1021/jf903332a
- PubMed:
- 20232890
-
http://dx.doi.org/10.1021/jf903332a
- Author:
- Kaur, Lovedeep; Rutherfurd, Shane M.; Moughan, Paul J.; Drummond, Lynley; Boland, Mike J.
- Source:
- Journal of agricultural and food chemistry 2010 v.58 no.8 pp. 5074–5080
- ISSN:
- 0021-8561
- Subject:
- cysteine proteinases; kiwifruit; small intestine; in vitro digestion; proteins; enzyme activity; dietary protein; pepsin
- Abstract:
- ... This paper describes an in vitro study that tests the proposition that actinidin from green kiwifruit influences the digestion of proteins in the small intestine. Different food proteins, from sources including soy, meat, milk, and cereals, were incubated in the presence or absence of green kiwifruit extract (containing actinidin) using a two-stage in vitro digestion system consisting of an incuba ...
- DOI:
- 10.1021/jf903835g
- PubMed:
- 20232891
-
http://dx.doi.org/10.1021/jf903835g
- Author:
- Smith, Alan M.; Jaime-Fonseca, Monica R.; Grover, Liam M.; Bakalis, Serafim
- Source:
- Journal of agricultural and food chemistry 2010 v.58 no.8 pp. 4719–4724
- ISSN:
- 0021-8561
- Subject:
- alginates; liposomes (artificial); encapsulation; alkaline phosphatase; functional properties; pH; gastric juice; novel foods; model food systems; proteins; oral administration; particle size; crosslinking
- Abstract:
- ... The present study has evaluated the potential of alginate-loaded liposomes as a vehicle for the oral delivery of bioactive proteins. The vesicles were prepared from the phospholipid dipalmatoyl phosphatidylcholine using a simple dry film hydration technique. Alkaline phosphatase (ALP) was used as a model bioactive protein, which was encapsulated in alginate-loaded liposomes and conventional liposo ...
- DOI:
- 10.1021/jf904466p
- PubMed:
- 20225816
-
http://dx.doi.org/10.1021/jf904466p
- Author:
- Pownall, Trisha L.; Udenigwe, Chibuike C.; Aluko, Rotimi E.
- Source:
- Journal of agricultural and food chemistry 2010 v.58 no.8 pp. 4712–4718
- ISSN:
- 0021-8561
- Subject:
- amino acid composition; antioxidant activity; peas; seeds; Pisum sativum; food analysis; food composition; enzymatic hydrolysis; protein hydrolysates; legume protein
- Abstract:
- ... The amino acid composition and antioxidant activities of peptide fractions obtained from HPLC separation of a pea protein hydrolysate (PPH) were studied. Thermolysin hydrolysis of pea protein isolate and ultrafiltration (3 kDa molecular weight cutoff membrane) yielded a PPH that was separated into five fractions (F1−F5) on a C18 reverse phase HPLC column. The fractions that eluted later from the c ...
- DOI:
- 10.1021/jf904456r
- PubMed:
- 20359226
-
http://dx.doi.org/10.1021/jf904456r
- Author:
- Zhao, Yang; Lee, Mao-Jung; Cheung, Connie; Ju, Ji-Hyeung; Chen, Yu-Kuo; Liu, Ba; Hu, Long-Qin; Yang, Chung S.
- Source:
- Journal of agricultural and food chemistry 2010 v.58 no.8 pp. 4844–4852
- ISSN:
- 0021-8561
- Subject:
- metabolites; tocopherols; tocotrienols; mice; animal models; humans; nutrition physiology; antioxidants; biomarkers; nutrition assessment
- Abstract:
- ... Tocopherols and tocotrienols, collectively known as vitamin E, are essential antioxidant nutrients. The biological fates and metabolite profiles of the different forms are not clearly understood. The objective of this study is to simultaneously analyze the metabolites of different tocopherols and tocotrienols in mouse and human samples. Using HPLC/electrochemical detection and mass spectrometry, 1 ...
- DOI:
- 10.1021/jf904464u
- PubMed:
- 20222730
- PubMed Central:
- PMC2858244
-
http://dx.doi.org/10.1021/jf904464u
- Author:
- Coulier, Leon; Bradley, Emma L.; Bas, Richard C.; Verhoeckx, Kitty C.M.; Driffield, Malcolm; Harmer, Nick; Castle, Laurence
- Source:
- Journal of agricultural and food chemistry 2010 v.58 no.8 pp. 4873–4882
- ISSN:
- 0021-8561
- Subject:
- model food systems; packaging materials; food analysis; coatings; chemical reactions; packaging materials migration; cans; food composition; canned foods; cysteine
- Abstract:
- ... Bisphenol A diglycidyl ether (BADGE) is an epoxide that is used as a starting substance in the manufacture of can coatings for food-contact applications. Following migration from the can coating into food, BADGE levels decay and new reaction products are formed by reaction with food ingredients. The significant decay of BADGE was demonstrated by liquid chromatographic (LC) analysis of foodstuffs, ...
- DOI:
- 10.1021/jf904160a
- PubMed:
- 20334396
-
http://dx.doi.org/10.1021/jf904160a
- Author:
- Gorjanovic, Stanislava Z.; Novakovic, Miroslav M.; Potkonjak, Nebojsa I.; Suznjevic, Desanka Z.
- Source:
- Journal of agricultural and food chemistry 2010 v.58 no.8 pp. 4626–4631
- ISSN:
- 0021-8561
- Subject:
- antioxidant activity; wines; wine quality; food analysis; food composition; assays; hydrogen peroxide; phenolic compounds
- Abstract:
- ... Antioxidant (AO) activity of various red and white wines of different origin as well as some individual phenolic compounds present in wine has been assessed using a polarographic assay. Direct current polarography has been used to survey hydrogen peroxide scavenge (HPS) upon gradual addition of tested samples. Results expressed as reciprocal value of wine volume required for 50% decrease of anodic ...
- DOI:
- 10.1021/jf100022e
- PubMed:
- 20302358
-
http://dx.doi.org/10.1021/jf100022e
- Author:
- Udenigwe, Chibuike C.; Aluko, Rotimi E.
- Source:
- Journal of agricultural and food chemistry 2010 v.58 no.8 pp. 4762–4768
- ISSN:
- 0021-8561
- Subject:
- antioxidants; peptidyl-dipeptidase A; enzyme inhibition; enzyme inhibitors; linseed; oilseed protein; food analysis; food composition; peptides; mixtures; protein hydrolysates; enzymatic hydrolysis; functional foods
- Abstract:
- ... Hydrolysis of flaxseed proteins using thermolysin and pronase followed by mixing with activated carbon, centrifugation and filtration yielded a filtrate (peptide mixture) with a Fischer ratio (branched-chain amino acids/aromatic amino acids) of 23.65 and a phenylalanine + tyrosine content of 1.11%. Gel permeation chromatography showed that the flaxseed peptide sample contained mainly low molecular ...
- DOI:
- 10.1021/jf100149w
- PubMed:
- 20218606
-
http://dx.doi.org/10.1021/jf100149w
- Author:
- Sandhu, Amandeep K.; Gu, Liwei
- Source:
- Journal of agricultural and food chemistry 2010 v.58 no.8 pp. 4681–4692
- ISSN:
- 0021-8561
- Subject:
- phenolic compounds; grape seeds; fruit pulp; grapes; Vitis rotundifolia; high performance liquid chromatography; mass spectrometry; food analysis; fruit composition; fruit quality
- Abstract:
- ... The objective of this study was to determine and compare the total phenolic content and antioxidant capacity in the seeds, skin, and pulp of eight cultivars of Florida-grown muscadine grapes and to identify the phenolic compounds in these respective portions. Total phenolic contents were determined colorimetrically using Folin-Ciocalteu reagent, and antioxidant capacity was determined by oxygen ra ...
- DOI:
- 10.1021/jf904211q
- PubMed:
- 20334341
-
http://dx.doi.org/10.1021/jf904211q
- Author:
- Pozo-Bayon, Maria Angeles; Pimenta, Paloma; Pilch, Shira; Masters, James G.; Martin-Alvarez, Pedro J.; Reineccius, Gary
- Source:
- Journal of agricultural and food chemistry 2010 v.58 no.8 pp. 5034-5041
- ISSN:
- 0021-8561
- Subject:
- mass spectrometry; models; odors; teeth; hygiene; equipment performance; flavor compounds
- Abstract:
- ... A mechanical tooth brushing device coupled to an atmosphere pressure ionization ion trap mass spectrometer (API-IT-MS) combination has been developed to study the influence of time and dilution on aroma release from a model dentifrice system. API-IT-MS response to nine commonly used dentifrice flavor components was initially studied. Linear regression models were developed based on an exponential ...
- DOI:
- 10.1021/jf904266y
- PubMed:
- 20345098
-
http://dx.doi.org/10.1021/jf904266y
- Author:
- Block, Eric A.; Dane, A. John; Thomas, Siji; Cody, Robert B.
- Source:
- Journal of agricultural and food chemistry 2010 v.58 no.8 pp. 4617–4625
- ISSN:
- 0021-8561
- Subject:
- organic sulfur compounds; food analysis; crushing; acids; garlic; Allium; equipment performance; food composition; mass spectrometry; detection
- Abstract:
- ... Through the use of direct analysis in real time mass spectrometry (DART-MS), 2-propenesulfenic acid, an intermediate long postulated as being formed when garlic (Allium sativum) is crushed, has been detected for the first time and determined by mass spectrometric methods to have a half-life of <1 s at room temperature. Two other key intermediates, 2-propenesulfinic acid and diallyl trisulfane S-ox ...
- DOI:
- 10.1021/jf1000106
- PubMed:
- 20225897
-
http://dx.doi.org/10.1021/jf1000106
- Author:
- Herrero, Beatriz; Madrinan, Maria; Vieites, Juan M.; Espineira, Montserrat
- Source:
- Journal of agricultural and food chemistry 2010 v.58 no.8 pp. 4794–4799
- ISSN:
- 0021-8561
- Subject:
- product authenticity; food analysis; Gadus morhua; polymerase chain reaction; seafoods; equipment performance; food composition; cod (fish); traceability
- Abstract:
- ... This work describes the development of a real-time polymerase chain reaction (RT-PCR) system for the detection and identification of Atlantic cod (Gadus morhua). Among the advantages of this technique, it is worth highlighting that this is reliable in terms of specificity and sensitivity. The TaqMan real-time PCR is the simplest, fastest testing process and has the highest potential for automation ...
- DOI:
- 10.1021/jf904018h
- PubMed:
- 20355721
-
http://dx.doi.org/10.1021/jf904018h
- Author:
- Yang, Hui; Figueroa, Mario; To, Satoshi; Baggett, Scott; Jiang, Bei; Basile, Margaret J.; Weinstein, Bernard; Kennelly, Edward J.
- Source:
- Journal of agricultural and food chemistry 2010 v.58 no.8 pp. 4749–4755
- ISSN:
- 0021-8561
- Subject:
- Garcinia; small fruits; fruit extracts; food analysis; fruit composition; cytotoxicity; human cell lines; functional foods
- Abstract:
- ... A series of 13 known compounds, including seven benzophenones [guttiferone A (1), guttiferone K (2), xanthochymol (3), guttiferone E (4), cycloxanthochymol (5), isoxanthochymol (6), and gambogenone (7)], five biflavonoids [amentoflavone (8), 3,8′′-biapigenin (9), (+)-volkensiflavone (10), (+)-morelloflavone (11), and (+)-fukugiside (12)], and the xanthone derivative alloathyriol (13), were identif ...
- DOI:
- 10.1021/jf9046094
- PubMed:
- 20345100
-
http://dx.doi.org/10.1021/jf9046094
- Author:
- Takoi, Kiyoshi; Koie, Koichiro; Itoga, Yutaka; Katayama, Yuta; Shimase, Masayuki; Nakayama, Yasuyuki; Watari, Junji
- Source:
- Journal of agricultural and food chemistry 2010 v.58 no.8 pp. 5050-5058
- ISSN:
- 0021-8561
- Subject:
- biotransformation; hops; monoterpenoids; alcohols; beers; brewers yeast; flavor; food analysis; food composition; cultivars; Humulus lupulus; enzyme activity; alcoholic fermentation
- Abstract:
- ... It is well-known that various beers contain many flavor compounds derived from barley malts, hops, yeast fermentation, and other raw materials. Among these flavor compounds, terpenoids are mainly derived from hops. Linalool, one of the monoterpene alcohols, has been found in various beers and been regarded as an important factor for a hop-derived beer flavor. We focus on contributions of other mon ...
- DOI:
- 10.1021/jf1000524
- PubMed:
- 20364865
-
http://dx.doi.org/10.1021/jf1000524
- Author:
- Kim, Seung-Hyun; Kelly, Peter B.; Clifford, Andrew J.
- Source:
- Journal of agricultural and food chemistry 2010 v.58 no.8 pp. 4632-4637
- ISSN:
- 0021-8561
- Subject:
- radiation resistance; nutrients; foods; dietary exposure; biopharmaceuticals; isotopes; carbon; isotope labeling; humans; metabolism
- Abstract:
- ... 14C has long been used as a tracer for quantifying the in vivo human metabolism of food components, biopharmaceuticals, and nutrients. Minute amounts (less than or equal to 1 x 10(-18) mol) of 14C can be measured with high-throughput 14C-accelerator mass spectrometry (HT 14C-AMS) in isolated chemical extracts of biological, biomedical, and environmental samples. Availability of in vivo human data ...
- DOI:
- 10.1021/jf100113c
- PubMed:
- 20349979
- PubMed Central:
- PMC2857889
-
http://dx.doi.org/10.1021/jf100113c
- Author:
- Escudero, Elizabeth; Sentandreu, Miguel Angel; Toldra, Fidel
- Source:
- Journal of agricultural and food chemistry 2010 v.58 no.8 pp. 5160–5165
- ISSN:
- 0021-8561
- Subject:
- pork; in vitro digestion; dietary protein; peptides; nutritive value; protein hydrolysates
- Abstract:
- ... The main objective of this work was to identify and characterize the peptides generated by simulated gastrointestinal digestion of pork meat (longissimus dorsi) by the sequential action of pepsin and pancreatin. The obtained hydrolysate was analyzed by liquid chromatography coupled to a quadrupole time-of-flight mass spectrometer equipped with a nanoelectrospray ionization source (nano LC−ESI-MS/M ...
- DOI:
- 10.1021/jf904535m
- PubMed:
- 20356084
-
http://dx.doi.org/10.1021/jf904535m
- Author:
- Goncalves, Any Elisa De Souza Schmidt; Lajolo, Franco Maria; Genovese, Maria Ines
- Source:
- Journal of agricultural and food chemistry 2010 v.58 no.8 pp. 4666–4674
- ISSN:
- 0021-8561
- Subject:
- food analysis; fruit composition; indigenous species; antioxidant activity; glycemic effect; tropical and subtropical fruits; fruit pulp; frozen fruit; functional foods; diabetes mellitus; phenolic compounds; alpha-glucosidase; enzyme inhibition; Brazil
- Abstract:
- ... Foods provide essential and bioactive compounds with health-promoting properties such as antioxidant, anti-inflammatory, and hypocholesterolemic activities, which have been related to vitamins A, C, and E and phenolic compounds such as flavonoids. Therefore, the aim of this work was to identify potential sources of bioactive compounds through the determination of flavonoids and ellagic acid conten ...
- DOI:
- 10.1021/jf903875u
- PubMed:
- 20337450
-
http://dx.doi.org/10.1021/jf903875u
- Author:
- Lee, Chen-Hsien; Hsu, Keng-Hao; Wang, Sheng-Yang; Chang, Tun-Tschu; Chu, Fang-Hua; Shaw, Jei-Fu
- Source:
- Journal of agricultural and food chemistry 2010 v.58 no.8 pp. 4800–4807
- ISSN:
- 0021-8561
- Subject:
- molecular cloning; lanosterol; genes; fungi; biosynthesis; recombinant proteins; gene expression; bioactive properties; lipid metabolism
- Abstract:
- ... Sterol 14α-demethylase (CYP51) is one of the key enzymes for sterol biosynthesis in fungi; it is widely distributed in all members of the cytochrome P450 superfamily. In this study, AcCyp51, encoding a cytochrome P450 sterol 14α-demethylase, was obtained from the sequences of EST libraries of Antrodia cinnamomea by using 5′ RACE and genome walking methods. The open reading frame of AcCyp51 is 1635 ...
- DOI:
- 10.1021/jf904257h
-
http://dx.doi.org/10.1021/jf904257h
- Author:
- Huang, Chih-Yu; Ken, Chuian-Fu; Chi, Hsiang-Hui; Wen, Lisa; Lin, Chi-Tsai
- Source:
- Journal of agricultural and food chemistry 2010 v.58 no.8 pp. 4825–4830
- ISSN:
- 0021-8561
- Subject:
- complementary DNA; glutathione-disulfide reductase; medicinal plants; amino acid sequences; gene expression; molecular cloning; mushrooms; antioxidant activity; sequence homology; oxidative stress
- Abstract:
- ... A cDNA encoding putative thioredoxin reductase (TR) was identified from a medicinal mushroom, Taiwanofungus camphorata (T. camphorata). Alignment of the deduced amino acid sequence with TRs from other organisms showed high levels of identity (59−74%). A three-dimensional (3-D) homology structure was created for this TR. Functional T. camphorata TR (TcTR) was overexpressed in yeast and purified. Th ...
- DOI:
- 10.1021/jf100222b
- PubMed:
- 20307095
-
http://dx.doi.org/10.1021/jf100222b
- Author:
- Wehr, J. Bernhard; Blamey, F. Pax C.; Menzies, Neal W.
- Source:
- Journal of agricultural and food chemistry 2010 v.58 no.8 pp. 4554–4559
- ISSN:
- 0021-8561
- Subject:
- seed germination; chemical composition; colorimetry; seeds; cation exchange capacity; copper; lanthanum; cell walls; chemical analysis; equipment performance; dyes; roots; shoots
- Abstract:
- ... The determination of the cation exchange capacity (CEC) of plant cell walls is important for many physiological studies. We describe the determination of cell wall CEC by cation binding, using either copper (Cu) or lanthanum (La) ions, and by colorimetry. Both cations are strongly bound by cell walls, permitting fast and reproducible determinations of the CEC of small samples. However, the dye bin ...
- DOI:
- 10.1021/jf100097k
- PubMed:
- 20345166
-
http://dx.doi.org/10.1021/jf100097k