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- Zeng, Run-Ying, et al. Show all 5 Authors
- Journal of agricultural and food chemistry 2016 v.64 no.38 pp. 7251-7258
- Escherichia coli; Flammeovirga; acid tolerance; amino acids; bacteria; beta-agarase; functional foods; genes; glycosides; industry; pH; temperature; thermal stability
- ... A novel gene (aga4436), encoding a potential agarase of 456 amino acids, was identified in the genome of deep-sea bacterium Flammeovirga sp. OC4. Aga4436 belongs to the glycoside hydrolase 16 β-agarase family. Aga4436 was expressed in Escherichia coli as a fusion protein and purified. Recombinant Aga4436 showed an optimum agarase activity at 50–55 °C and pH 6.5, with a wide active range of tempera ...