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- Zou, Huijun, et al. Show all 8 Authors
- Journal of agricultural and food chemistry 2018 v.66 no.34 pp. 9061-9069
- Saccharomyces cerevisiae; carbon; catabolite repression; ethyl carbamate; evolutionary adaptation; food production; genes; genetic engineering; genetic variation; genomics; nitrogen; nitrogen metabolism; rice wines; screening; transcription (genetics); urea; wine yeasts
- ... Urea is the major precursor of ethyl carbamate in Chinese rice wine. Although efforts have been made to decrease urea accumulation, few methods can be applied to industrial food production due to potential safety concerns. In this study, adaptive laboratory evolution (ALE) followed by high-throughput screening was used to identify low urea-accumulating strains derived from the industrial Chinese r ...
- Zou, Huijun, et al. Show all 7 Authors
- Journal of agricultural and food chemistry 2017 v.65 no.8 pp. 1641-1648
- Saccharomyces cerevisiae; arginine; carcinogens; endocytosis; engineering; ethyl carbamate; glutamine; lysine; models; mutants; mutation; nitrogen; plasma membrane; rice wines; transporters; ubiquitination; urea; yeasts
- ... Ubiquitination can significantly affect the endocytosis and degradation of plasma membrane proteins. Here, the ubiquitination of a Saccharomyces cerevisiae urea plasma membrane transporter (Dur3p) was altered. Two potential ubiquitination sites, lysine residues K556 and K571, of Dur3p were predicted and replaced by arginine, and the effects of these mutations on urea utilization and formation unde ...