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You searched for: Journal name Journal of applied microbiology Remove constraint Journal name: Journal of applied microbiology Publication year rev 7980-2020 Remove constraint Publication year rev: 7980-2020 Source 2020 v.129 no.6 Remove constraint Source: 2020 v.129 no.6
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1. Chicory fibre improves reproductive performance of pregnant rats involving in altering intestinal microbiota composition

Author:
Y. Hu; X. Huang; X. Zong; Z. Bi; Y. Cheng; X. Xiao; F. Wang; Y. Wang; Z. Lu
Source:
Journal of applied microbiology 2020 v.129 no.6 pp. 1693-1705
ISSN:
1364-5072
Subject:
Bacteroidetes; Firmicutes; Proteobacteria; acetic acid; animal husbandry; antimicrobial peptides; antinutritional factors; birth weight; butyric acid; cecum; chicory; dietary fiber; fructans; insoluble fiber; intestinal microorganisms; mucins; mycotoxins; pregnancy; probiotics; propionic acid; reproductive performance; tight junctions
Abstract:
... AIM: Chicory fibre (CF) is rich in fructan, which always functions as a quality dietary fibre source during mammalian pregnancy; however, its effect on reproductive performance remains unclear. METHODS AND RESULTS: 40 pregnant SD rats were randomly allotted to receive one of four diets: basal diet (control group), basal diet + 5% CF, basal diet + 10% CF, and basal diet + 15% CF, respectively. We f ...
DOI:
10.1111/jam.14679

2. Effect of salt concentration on microbial communities, physicochemical properties and metabolite profile during spontaneous fermentation of Chinese northeast sauerkraut

Author:
X. Yang; W. Hu; Z. Xiu; A. Jiang; G. Saren; Y. Ji; Y. Guan; K. Feng
Source:
Journal of applied microbiology 2020 v.129 no.6 pp. 1458-1471
ISSN:
1364-5072
Subject:
fermentation; glucose; lactic acid; metabolites; metabolome; pH; principal component analysis; salt concentration; sauerkraut; sensory evaluation
Abstract:
... AIM: The aim of this study was to study the effects of salt concentrations on the microbial communities, physicochemical properties, metabolome profiles and sensory characteristics during the fermentation of traditional northeast sauerkraut. METHODS AND RESULTS: Northeast sauerkraut was spontaneously fermented under four salt concentrations (0·5, 1·5, 2·5 and 3·5%, w/w). The result of microbiologi ...
DOI:
10.1111/jam.14786

3. Identification of Vibrio alginolyticus virulent strain‐specific DNA regions by suppression subtractive hybridization and PCR

Author:
Z.‐Y. Xie; X.‐X. Gong; X.‐D. Xu; B. Mei; X.‐Z. Xuan; H. Long; X. Zhang; X.‐N. Cai
Source:
Journal of applied microbiology 2020 v.129 no.6 pp. 1472-1485
ISSN:
1364-5072
Subject:
Vibrio alginolyticus; avirulent strains; chemotaxis; farmed fish; genome; microbiology; pathogens; rapid methods; suppression subtractive hybridization; transcription factors; type VI secretion system; virulence; virulent strains; China
Abstract:
... AIMS: Vibrio alginolyticus was frequently isolated from diseased farmed fish in the coaster waters of Hainan Island over the past two decades. In this study, we attempted to identify candidates of virulent strain‐specific DNA regions for this pathogen. METHODS AND RESULTS: Suppression subtractive hybridization (SSH) and PCR were successively performed between the typical virulent strain and avirul ...
DOI:
10.1111/jam.14739

4. Immunosensor‐based rapid quantitative detection of Newcastle disease virus antibodies using innovative gold immunochromatographic assay

Author:
F. Yang; Y. Li; X. Jin; Q. Xu; F. Cheng; X. Wang
Source:
Journal of applied microbiology 2020 v.129 no.6 pp. 1751-1757
ISSN:
1364-5072
Subject:
Avian orthoavulavirus 1; antibodies; antibody detection; blood serum; detection limit; gold; hemagglutination inhibition test; immunoaffinity chromatography; immunosensors; microbiology; quantitative analysis
Abstract:
... AIMS: A novel quantitative method for rapid Newcastle disease virus (NDV) antibody detection was developed based on an innovative gold immunochromatographic assay with a quantitative immunosensor. METHODS AND RESULTS: NDV antibody‐detecting test strips containing a two‐reaction system and double‐test lines (T1, T2) were prepared. The test results were judged according to the signal ratio between t ...
DOI:
10.1111/jam.14688

5. Improvement of gibberellin production by a newly isolated Fusarium fujikuroi mutant

Author:
B. Zhang; Z. Lei; Z.‐Q. Liu; Y.‐G. Zheng
Source:
Journal of applied microbiology 2020 v.129 no.6 pp. 1620-1632
ISSN:
1364-5072
Subject:
Fusarium fujikuroi; biosynthesis; endocytosis; fermentation; genome; histone code; lithium chloride; microbiology; monitoring; mutants; sequence analysis
Abstract:
... AIMS: To obtain and investigate the potential mechanism for GA₃ production in Fusarium fujikuroi GA‐251, a high GA₃ producer. METHODS AND RESULTS: Fusarium fujikuroi IMI 58289 was bred with Cobalt‐60 (⁶⁰Co) radiation and lithium chloride treatment. The best mutant strain GA‐251 was obtained for the subsequent optimization of fermentation conditions. The yield of GA₃ by GA‐251 was 2100 mg l⁻¹, whil ...
DOI:
10.1111/jam.14746

6. Lysobacter enzymogenes LE16 autolysates have potential as biocontrol agents—Lysobacter sp. autolysates as biofungicide

Author:
D.M. Chen; H.J. Yang; J.G. Huang; L. Yuan
Source:
Journal of applied microbiology 2020 v.129 no.6 pp. 1684-1692
ISSN:
1364-5072
Subject:
Alternaria alternata; Didymella bryoniae; Glomerella cingulata; Lysobacter enzymogenes; Penicillium italicum; Phytophthora capsici; Phytophthora nicotianae; Pseudomonas syringae pv. tabaci; Sclerotinia sclerotiorum; Staphylococcus aureus; Thanatephorus cucumeris; ambient temperature; animal pathogenic bacteria; biological control; biological control agents; blight; culture media; databases; disease incidence; greenhouse experimentation; lysozyme; pepper; plant pathogenic bacteria; proteinases; secondary metabolites; siderophores; soil
Abstract:
... AIMS: Biological techniques can manage plant diseases safely and in environmentally friendly ways, but their efficacy needs improvement. It is of the utmost importance to search for powerful microbes for the effective control of plant diseases. METHODS AND RESULTS: Unheated self‐digestive solutions (SDS) that were heated at 100°C for 30 min(H‐SDS) or stored for 12 months at room temperature (S‐SDS ...
DOI:
10.1111/jam.14752

7. Novel amidase catalysed process for the synthesis of vorinostat drug

Author:
R.V. Singh; H. Sharma; A. Ganjoo; A. Kumar; V. Babu
Source:
Journal of applied microbiology 2020 v.129 no.6 pp. 1589-1597
ISSN:
1364-5072
Subject:
Bacillus smithii; amidase; biotransformation; catalytic activity; drugs; ethanol; histone deacetylase; microbiology; pH; potassium phosphates; solvents
Abstract:
... AIM: Presently, N‐hydroxy‐N′‐phenyloctanediamide (vorinostat) which is an effective histone deacetylase inhibitor, is being synthesized chemically. Hence, present study aims to develop an eco‐friendly approach for the synthesis of vorinostat from N′‐phenyloctanediamide through biotransformation. METHODS AND RESULTS: Using the amidase of Bacillus smithii IIIMB2907 in time course conversion and orga ...
DOI:
10.1111/jam.14753

8. Overall changes in the transcriptome of Escherichia coli O26:H11 induced by a subinhibitory concentration of ciprofloxacin

Author:
C. Valat; E. Hirchaud; A. Drapeau; F. Touzain; C. de Boisseson; M. Haenni; Y. Blanchard; J.‐Y. Madec
Source:
Journal of applied microbiology 2020 v.129 no.6 pp. 1577-1588
ISSN:
1364-5072
Subject:
Escherichia coli O26; amino acids; bacteriophages; cattle; ciprofloxacin; gene expression regulation; gene ontology; genomics; hydrolases; transcription (genetics); transcriptome; transcriptomics; transposases
Abstract:
... AIMS: The goal was to explore the effects of subinhibitory concentration (SIC) (0·5 MIC = 20 µg l⁻¹) of ciprofloxacin on the transcriptome of enterohaemorrhagic Escherichia coli O26:H11 isolate by 60 minutes of exposure. MATERIALS AND RESULTS: We used a combination of comparative genomic and transcriptomic (RNAseq) analyses. The whole genome of the E. coli O26:H11 #30934 strain of bovine origin wa ...
DOI:
10.1111/jam.14741

9. Prior exposure of agriculture cephalosporin ceftiofur impaired conjugation of blaCTX‐M‐65 gene‐bearing plasmid in Salmonella Saintpaul

Author:
Y.H. Zwe; H.G. Yuk
Source:
Journal of applied microbiology 2020 v.129 no.6 pp. 1552-1565
ISSN:
1364-5072
Subject:
Salmonella Saintpaul; ceftiofur; ceftriaxone; enzyme activity; gene expression; genes; microbiology; phenotype; plasmids
Abstract:
... AIMS: Although a link between agricultural cephalosporin use and resistance in Salmonella has been demonstrated with the drug ceftiofur, the underlying mechanism of the correlation is unclear. This study investigated the impact of ceftiofur exposure in S. Saintpaul on ceftriaxone resistance, the gene expression and the conjugative transfer of the blaCTX‐M‐₆₅ gene. METHODS AND RESULTS: Prior ceftio ...
DOI:
10.1111/jam.14749

10. Role of a 21‐kDa iron‐regulated protein IrpA in the uptake of ferri‐exochelin by Mycobacterium smegmatis

Author:
N. Kumar; M. Sritharan
Source:
Journal of applied microbiology 2020 v.129 no.6 pp. 1733-1743
ISSN:
1364-5072
Subject:
Mycobacterium smegmatis; Mycobacterium tuberculosis; iron; pathogens; proteinases; siderophores; structural proteins
Abstract:
... AIMS: To characterize the 21‐kDa iron‐regulated cell wall protein in Mycobacterium smegmatis co‐expressed with the siderophores mycobactin, exochelin and carboxymycobactin upon iron limitation. METHODS AND RESULTS: Mycobacterium smegmatis, grown in the presence of 0·02 μg Fe ml⁻¹ (low iron) produced high levels of all the three siderophores, which were repressed in bacteria supplemented with 8 μg  ...
DOI:
10.1111/jam.14728

11. Screening the antifungal activities of monoterpenes and their isomers against Candida species

Author:
A. Iraji; S. Yazdanpanah; F. Alizadeh; S. Mirzamohammadi; Y. Ghasemi; K. Pakshir; Y. Yang; K. Zomorodian
Source:
Journal of applied microbiology 2020 v.129 no.6 pp. 1541-1551
ISSN:
1364-5072
Subject:
Candida tropicalis; antifungal agents; antifungal properties; camphor; candidiasis; carvone; citronellol; cosmetics; limonene; menthol; menthone; minimum inhibitory concentration; odors; secondary metabolites; statistical analysis; taste
Abstract:
... AIM: Increasing the occurrence of non‐albicans Candida species with intrinsic or acquired resistance to antifungals as well as the emergence of multidrug Candida species coupled with the limited antifungal agents challenges the treatment of candidiasis. Consequently, a class of secondary metabolites of plants exhibiting decent antifungal activity. Therefore, this study aimed to evaluate the antifu ...
DOI:
10.1111/jam.14740

12. Technological variables influencing the growth and stability of a silage inoculant based on spray‐dried lactic acid bacteria

Author:
J.E. Blajman; G. Vinderola; A. Cuatrin; M.S. Lingua; R.B. Páez
Source:
Journal of applied microbiology 2020 v.129 no.6 pp. 1486-1496
ISSN:
1364-5072
Subject:
Lactobacillus plantarum; Pediococcus acidilactici; air flow; biomass production; commercialization; culture media; economic feasibility; fermentation; growth performance; lactic acid; pH; silage; solubility; spray drying; storage quality; viability; water content; whey protein concentrate
Abstract:
... AIMS: To investigate the influence of different culture media and fermentation conditions on growth performance and viability of three lactic acid bacteria (LAB) strains with potential as silage inoculants, and to optimize spray‐drying in order to enhance survival to dehydration and storage stability. METHODS AND RESULTS: In house‐formulated MRS was a suitable low‐cost culture medium for Lactobaci ...
DOI:
10.1111/jam.14750

13. γ‐aminobutyric acid production by Kluyveromyces marxianus strains

Author:
G. Perpetuini; F. Tittarelli; N. Battistelli; G. Suzzi; R. Tofalo
Source:
Journal of applied microbiology 2020 v.129 no.6 pp. 1609-1619
ISSN:
1364-5072
Subject:
Kluyveromyces marxianus; anabolism; biodiversity; catabolism; gene expression; high performance liquid chromatography; nucleotide sequences; phenotype; succinic acid; yeasts
Abstract:
... AIMS: Kluyveromyces marxianus dairy strains were tested for γ‐aminobutyric acid (GABA) production. The genes involved in GABA catabolism (UGA1 and UGA2) and anabolism (GAD1) were found in K. marxianus genome. Their relative expression was evaluated with primer designed ad hoc. METHODS AND RESULTS: Strains were grouped on the basis of GAD1 gene sequence. Representative strains for each group were t ...
DOI:
10.1111/jam.14736

14. Antibiotic‐induced role interchange between rare and predominant bacteria retained the function of a bacterial community for denitrifying quinoline degradation

Author:
X. Zhang; G. Chen; S. Zhong; T. Wang; M. Ji; X. Wu
Source:
Journal of applied microbiology 2020 v.129 no.6 pp. 1598-1608
ISSN:
1364-5072
Subject:
Achromobacter; Comamonas; Pseudomonas; Pseudoxanthomonas; Thauera; bacterial communities; biodiversity; bioreactors; denitrification; environmental factors; genes; nitrates; pollutants; quinoline; streptomycin; wastewater treatment
Abstract:
... AIM: Quinoline is a recalcitrant pollutant in coking wastewater which has been broadly investigated with many isolates possessing aerobic quinoline‐degrading ability. However, studies on anaerobic degradation and the corresponding bacteria are very scarce. This study attempted to investigate the role of diverse functional members and the redundancy of quinoline degradation in a lab‐scale quinoline ...
DOI:
10.1111/jam.14755

15. Ecological and biotechnological importance of secondary metabolites produced by coral‐associated bacteria

Author:
F. Modolon; A.R. Barno; H.D.M. Villela; R.S. Peixoto
Source:
Journal of applied microbiology 2020 v.129 no.6 pp. 1441-1457
ISSN:
1364-5072
Subject:
antiparasitic agents; corals; homeostasis; secondary metabolites
Abstract:
... Symbiotic relationships between corals and their associated micro‐organisms are essential to maintain host homeostasis. Coral‐associated bacteria (CAB) can have different beneficial roles in the coral metaorganism, such as metabolizing essential nutrients for the coral host and protecting the coral from pathogens. Many CAB exert these functions via secondary metabolites, which include antibacteria ...
DOI:
10.1111/jam.14766

16. Effects of heating and recovery media pH on the heat resistance of Alicyclobacillus acidoterrestris Ad 746 spores

Author:
I. Leguerinel; M. Maucotel; T. Arnoux; M. Gaspari; N. Desriac; C. Chatzitzika; V.P. Valdramidis
Source:
Journal of applied microbiology 2020 v.129 no.6 pp. 1674-1683
ISSN:
1364-5072
Subject:
Alicyclobacillus acidoterrestris; fruits; heat; heat tolerance; microbiology; pH; spores
Abstract:
... AIMS: Alicyclobacillus acidoterrestris is a sporulating, acidophilic bacterial species which spoils acidic beverages such as fruit juices. This work aims to quantify the heat resistance of A. acidoterrestris spores and their recovery potential as a function of heating and recovery media pH. METHODS AND RESULTS: The heat treatments were carried out with the strain of A. acidoterrestris Ad 746 in Ba ...
DOI:
10.1111/jam.14745

17. Elucidation of fungal dye‐decolourizing peroxidase (DyP) and ligninolytic enzyme activities in decolourization and mineralization of azo dyes

Author:
G. Rajhans; S.K. Sen; A. Barik; S. Raut
Source:
Journal of applied microbiology 2020 v.129 no.6 pp. 1633-1643
ISSN:
1364-5072
Subject:
Geotrichum candidum; decolorization; enzyme activity; fungi; laccase; lignin peroxidase; methyl orange; mineralization; pH; peroxidase; temperature; textile mill effluents
Abstract:
... AIM: The aim of the study is to investigate the efficiency of Geotrichum candidum in the decolourization and mineralization of synthetic azo dyes. METHODS AND RESULTS: It includes screening of enzymes from G. candidum and its optimization, followed by decolourization and mineralization studies. Decolourization was observed to be maximum in methyl orange (94·6%) followed by Congo red (85%), trypan ...
DOI:
10.1111/jam.14731

18. Histamine and cholesterol lowering abilities of lactic acid bacteria isolated from artisanal Pico cheese

Author:
M.F.P. Domingos‐Lopes; C. Stanton; R.P. Ross; C.C.G. Silva
Source:
Journal of applied microbiology 2020 v.129 no.6 pp. 1428-1440
ISSN:
1364-5072
Subject:
Lactobacillus paracasei; adhesion; bile; cheeses; cholesterol; histamine; intestines; lactic acid; probiotics; raw milk; traditional technology; virulence
Abstract:
... AIMS: This study was designed to select lactic acid bacteria with histamine‐ and cholesterol‐reducing abilities to be used as potential probiotics. METHODS AND RESULTS: Thirty strains of lactic acid bacteria isolated from an artisanal raw milk cheese were screened for their abilities to degrade histamine, reduce cholesterol and hydrolyse bile salts. Strains were also screened for safety and probio ...
DOI:
10.1111/jam.14733

19. Identification and characterization of novel transglycosylating α‐glucosidase from Aspergillus neoniger

Author:
S. Kumar; A. Basu; K.A. Anu‐Appaiah; B.S. Gnanesh Kumar; S. Mutturi
Source:
Journal of applied microbiology 2020 v.129 no.6 pp. 1644-1656
ISSN:
1364-5072
Subject:
Aspergillus niger; asparagine; directed evolution; fungi; genes; glycosylation; hydrolysis; internal transcribed spacers; models; polyacrylamide gel electrophoresis; prebiotics; sequence analysis; soil; threonine
Abstract:
... AIM: Aspergillus niger is well established for secreting α‐glucosidase having transglycosylation activity, which is used as processing aid for synthesis of isomaltooligosaccharides. The present study focuses on identification and characterization of a non‐niger Aspergillus isolate and its gene conferring strong transglycosylation activity. METHODS AND RESULTS: The soil isolate was identified as As ...
DOI:
10.1111/jam.14757

20. Killing of bacterial spores by dodecylamine and its effects on spore inner membrane properties

Author:
S. Mokashi; J. Kanaan; D.L. Craft; B. Byrd; B. Zenick; M. Laue; G. Korza; W.W.K. Mok; P. Setlow
Source:
Journal of applied microbiology 2020 v.129 no.6 pp. 1511-1522
ISSN:
1364-5072
Subject:
Bacillus subtilis; microbiology; permeability; propidium; viability
Abstract:
... AIMS: The objective of this study was to determine the effects of Ca‐dipicolinic acid (CaDPA), cortex‐lytic enzymes (CLEs), the inner membrane (IM) CaDPA channel and coat on spore killing by dodecylamine. METHODS AND RESULTS: Bacillus subtilis spores, wild‐type, CaDPA‐less due to the absence of DPA synthase or the IM CaDPA channel, or lacking CLEs, were dodecylamine‐treated and spore viability and ...
DOI:
10.1111/jam.14732