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You searched for: Journal Journal of applied microbiology Remove constraint Journal: Journal of applied microbiology Publication Year 2019 Remove constraint Publication Year: 2019 Source 2019 v.126 no.6 Remove constraint Source: 2019 v.126 no.6
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1. A disclosure gel for visual detection of live Bacillus anthracis spores

Author:
Robinson, C.V.; Bishop, A.H.
Source:
Journal of applied microbiology 2019 v.126 no.6 pp. 1700-1707
ISSN:
1364-5072
Subject:
Bacillus anthracis; coatings; decontamination; enzyme activity; equipment; fluorescence; gels; hyaluronic acid; prioritization; risk; spores
Abstract:
... AIMS: To develop a gel formulation to trigger a visual signal for rapid disclosure of the location and extent of surface contamination with viable Bacillus anthracis spores. METHODS AND RESULTS: Methylumbelliferyl‐α‐d‐glucopyranoside was combined with hyaluronic acid to produce a gel that could be applied to a surface as a coating. It remained hydrated for a sufficient time for α‐glucosidase activ ...
DOI:
10.1111/jam.14226

2. Alicycliphilus: current knowledge and potential for bioremediation of xenobiotics

Author:
Solís‐González, C.J.; Loza‐Tavera, H.
Source:
Journal of applied microbiology 2019 v.126 no.6 pp. 1643-1656
ISSN:
1364-5072
Subject:
acetone; anaerobic conditions; anthracenes; bacteria; bacterial communities; biodegradation; bioremediation; chromium; denitrification; foams; genetic engineering; landfills; metabolism; nitrates; nonylphenols; oxygen; oxytetracycline; pollutants; polyurethanes; quinoline; sewage; sludge; solvents; tetracycline; toluene; triclosan; wastewater treatment; xenobiotics
Abstract:
... Alicycliphilus is a promising candidate for participating in the development of novel xenobiotics bioremediation processes. Members of the Alicycliphilus genus are environmental bacteria mostly found in polluted sites such as landfills and contaminated watercourses, and in sewage sludges from wastewater treatment plants. They exhibit a versatile metabolism and the ability to use oxygen, nitrate an ...
DOI:
10.1111/jam.14207

3. An alkaliphilic bacterium BKH4 of Bakreshwar hot spring pertinent to bioconcrete technology

Author:
Sarkar, A.; Chatterjee, A.; Mandal, S.; Chattopadhyay, B.
Source:
Journal of applied microbiology 2019 v.126 no.6 pp. 1742-1750
ISSN:
1364-5072
Subject:
Gram-positive bacteria; Lysinibacillus; compression strength; concrete; durability; hot springs; microbial communities; pH; ultrasonics
Abstract:
... AIMS: Hot springs have always drawn attention due to their unique chemical richness and the presence of different microbial communities. The use of hot spring bacteria in concrete technology is our primary focus; isolation of an alkaliphilic bacterium from the Bakreshwar hot springs having longer survival and better efficacy towards cementitious environment was the basis of our study's design. MET ...
DOI:
10.1111/jam.14236

4. Antibiofilm potential of purified environmental bacteriophage preparations against early stage Pseudomonas aeruginosa biofilms

Author:
Issa, R.; Chanishvili, N.; Caplin, J.; Kakabadze, E.; Bakuradze, N.; Makalatia, K.; Cooper, I.
Source:
Journal of applied microbiology 2019 v.126 no.6 pp. 1657-1667
ISSN:
1364-5072
Subject:
Pseudomonas aeruginosa; adsorption; antibiotic resistance; antibiotics; bacteria; bacteriophages; biofilm; ciprofloxacin; host strains; morphs; pyocyanin; virulence
Abstract:
... AIMS: This paper presents the potential of environmentally sourced bacteriophages to affect the growth of clinical isolates of Pseudomonas aeruginosa biofilms, and assesses the respective plaque morphotypes presented by each bacteriophage, in vitro. METHODS AND RESULTS: Bacterial host strains were typed for their ability to produce the quorum sensing‐controlled virulence factor pyocyanin, and then ...
DOI:
10.1111/jam.14241

5. Azospirillum brasilense Az39, a model rhizobacterium with AHL quorum‐quenching capacity

Author:
Gualpa, J.; Lopez, G.; Nievas, S.; Coniglio, A.; Halliday, N.; Cámara, M.; Cassán, F.
Source:
Journal of applied microbiology 2019 v.126 no.6 pp. 1850-1860
ISSN:
1364-5072
Subject:
Agrobacterium radiobacter; Azospirillum brasilense; Chromobacterium violaceum; Rhizobium; bacteria; bioinformatics; crops; enzymes; lactones; liquid chromatography; models; quorum sensing; spectroscopy
Abstract:
... AIMS: The aim of this research was to analyse the quorum‐sensing (QS) and quorum‐quenching (QQ) mechanisms based on N‐acyl‐l‐homoserine lactones (AHLs) in Azospirillum brasilense Az39, a strain with remarkable capacity to benefit a wide range of crops under agronomic conditions. METHODS AND RESULTS: We performed an in silico and in vitro analysis of the quorum mechanisms in A. brasilense Az39. The ...
DOI:
10.1111/jam.14269

6. Bacterial community composition under long‐term reduced tillage and no till management

Author:
Tyler, Heather L.
Source:
Journal of applied microbiology 2019 v.126 no.6 pp. 1797-1807
ISSN:
1364-5072
Subject:
agricultural land; bacterial communities; community structure; crop residues; disturbed soils; enzyme activity; genes; microbial biomass; no-tillage; organic matter; reduced tillage; ribosomal RNA; soil microorganisms; soil quality
Abstract:
... AIMS: The purpose of this study was to assess impacts of long‐term reduced tillage and no till management on bacterial communities in agricultural field soils. METHODS AND RESULTS: Samples from surface soils were collected from field plots maintained under reduced tillage and no till conditions for 14 years. No till soils had significantly higher microbial biomass, as well as β‐glucosidase activit ...
DOI:
10.1111/jam.14267

7. Bacterial species and total bacterial load in the distal oesophagus in patients with and without clinical gastric reflux

Author:
Kayar Dogan, E.; Abaci Gunyar, O.; Topal, F.; Alper, E.; Ekinci, N.
Source:
Journal of applied microbiology 2019 v.126 no.6 pp. 1891-1898
ISSN:
1364-5072
Subject:
DNA; adenocarcinoma; bacteria; biopsy; dysbiosis; esophagus; gastroesophageal reflux; histology; humans; microbial load; patients; people; polymerase chain reaction
Abstract:
... AIMS: The purpose of this study is to compare distal oesophagus of persons with and without gastric reflux in terms of bacterial load and presence of certain bacterial species. METHODS AND RESULTS: Two biopsy specimens were obtained from the distal oesophagus at 5 cm above the gastroesophageal junction of each of the 50 patients (20 with normal oesophagus and 30 with reflux oesophagitis) under end ...
DOI:
10.1111/jam.14250

8. Comparison of the gut microbiota composition between the wild and captive Tibetan wild ass (Equus kiang)

Author:
Gao, H.; Chi, X.; Qin, W.; Wang, L.; Song, P.; Cai, Z.; Zhang, J.; Zhang, T.
Source:
Journal of applied microbiology 2019 v.126 no.6 pp. 1869-1878
ISSN:
1364-5072
Subject:
Bacteroidetes; Equus kiang; Firmicutes; Spirochaetes; Tenericutes; animal health; asses; disease prevalence; ex situ conservation; feces; growth performance; high-throughput nucleotide sequencing; intestinal microorganisms; intestines; nutrition; ribosomal RNA; risk; wild animals; wildlife; winter
Abstract:
... AIMS: The gut microbiota has a great effect on the health and nutrition of the host. Manipulation of the intestinal microbiota may improve animal health and growth performance. The objectives of our study were to characterize the faecal microbiota between wild and captive Tibetan wild asses and discuss the differences and their reasons. METHODS AND RESULTS: Through high‐throughput sequencing of th ...
DOI:
10.1111/jam.14240

9. Composts of poultry litter or dairy manure differentially affect survival of enteric bacteria in fields with spinach

Author:
Neher, D.A.; Cutler, A.J.; Weicht, T.R.; Sharma, M.; Millner, P.D.
Source:
Journal of applied microbiology 2019 v.126 no.6 pp. 1910-1922
ISSN:
1364-5072
Subject:
Escherichia coli; ammonium nitrogen; bioavailability; biomass; biotic factors; community structure; compost extracts; dairy manure; field experimentation; fruits; intestinal microorganisms; laboratory experimentation; microbial communities; nutrients; poultry; poultry manure; risk assessment; soil; soil amendments; spinach; vegetable growing; vermicomposts
Abstract:
... AIMS: The aim was to determine the survival and persistence of Escherichia coli in soil amended with compost from different manure sources. METHOD AND RESULTS: Complex interactions of abiotic and biotic factors on E. coli survival were characterized in field experiment plots receiving randomly assigned compost treatments: dairy windrow, dairy vermicompost, poultry windrow or no compost. Biomass, a ...
DOI:
10.1111/jam.14268

10. Development and field testing of low‐cost, quantal microbial assays with volunteer reporting as scalable means of drinking water safety estimation

Author:
Loo, A.; Bivins, A.; John, V.; Becker, S.; Evanchec, S.; George, A.; Hernandez, V.; Mullaney, J.; Tolentino, L.; Yoo, R.; Nagarnaik, P.; Labhasetwar, P.; Brown, J.
Source:
Journal of applied microbiology 2019 v.126 no.6 pp. 1944-1954
ISSN:
1364-5072
Subject:
Escherichia coli; Klebsiella; drinking water; infrastructure; laboratory techniques; longitudinal studies; microfiltration; risk; risk estimate; rural areas; sanitation; villages; water quality; India
Abstract:
... AIMS: To evaluate a low‐cost water quality test for at‐scale drinking water safety estimation in rural India. METHODS AND RESULTS: Within a longitudinal study to characterize variability in household drinking water safety in rural Maharashtra, we piloted a low‐cost presence–absence (LCPA) microbial test designed to be used by volunteer residents in rural areas. In comparing the LCPA results with s ...
DOI:
10.1111/jam.14253

11. Effect of quorum sensing molecules and natamycin on biofilms of Candida tropicalis and other yeasts isolated from industrial juice filtration membranes

Author:
Agustín, M.d.R.; Viceconte, F.R.; Vela Gurovic, M.S.; Costantino, A.; Brugnoni, L.I.
Source:
Journal of applied microbiology 2019 v.126 no.6 pp. 1808-1820
ISSN:
1364-5072
Subject:
Candida kefyr; Candida krusei; Candida tropicalis; Rhodotorula rubra; adhesion; beverages; biofilm; cleaning agents; farnesol; fluorescence microscopy; food industry; fruit juices; gas chromatography-mass spectrometry; headspace analysis; natamycin; phenylethyl alcohol; plate count; quorum sensing; solid phase microextraction; solvents; stainless steel; ultrafiltration; yeasts
Abstract:
... AIMS: Cells limit the cell number of dense biofilms by releasing self‐inhibitory molecules. Here, we aim to assess the effectiveness of yeast quorum sensing (QS) molecules and the antifungal agent natamycin against yeast biofilms of strains commonly isolated from fruit juice ultrafiltration membranes. METHODS AND RESULTS: Yeast QS molecules, such as tyrosol, 2‐phenylethanol and farnesol, were dete ...
DOI:
10.1111/jam.14248

12. Efficient production of Pseudoionone with multipathway engineering in Escherichia coli

Author:
Jiang, R.; Chen, X.; Lian, J.; Huang, L.; Cai, J.; Xu, Z.
Source:
Journal of applied microbiology 2019 v.126 no.6 pp. 1751-1760
ISSN:
1364-5072
Subject:
Cucumis melo; Escherichia coli; NADP (coenzyme); bioprocess engineering; bioprocessing; chromosomes; culture flasks; enzymes; genes; lycopene; metabolic engineering; pentose phosphate cycle; tricarboxylic acid cycle
Abstract:
... AIMS: This study aimed to achieve efficient production of pseudoionone in Escherichia coli. METHODS AND RESULTS: Firstly, a stable lycopene‐producing strain was constructed by integrating lycopene biosynthetic pathway into the chromosome. Further introduction of the Cucumis melo carotenoid cleavage dioxygenase 1 (cmCCD1) gene resulted in the production of pseudoionone. Then, the tricarboxylic acid ...
DOI:
10.1111/jam.14245

13. Engineering Bacillus for efficient production of heterologous protein: current progress, challenge and prospect

Author:
Cai, D.; Rao, Y.; Zhan, Y.; Wang, Q.; Chen, S.
Source:
Journal of applied microbiology 2019 v.126 no.6 pp. 1632-1642
ISSN:
1364-5072
Subject:
Bacillus (bacteria); RNA; cell growth; engineering; enzymes; genes; heterologous gene expression; secretion; spores; transcription factors
Abstract:
... Bacillus species are widely recognized as good industrial platform strains, which can produce a variety of valuable products including enzymes and functional proteins. Bacillus expression systems gain various competitive edges over other expression systems, with respect to nontoxicity, convenience for gene modification and high yield of target proteins. Recently, a number of Bacillus expression sy ...
DOI:
10.1111/jam.14192

14. Essential oils inhibit the bovine respiratory pathogens Mannheimia haemolytica, Pasteurella multocida and Histophilus somni and have limited effects on commensal bacteria and turbinate cells in vitro

Author:
Amat, S.; Baines, D.; Timsit, E.; Hallewell, J.; Alexander, T.W.
Source:
Journal of applied microbiology 2019 v.126 no.6 pp. 1668-1682
ISSN:
1364-5072
Subject:
Haemophilus somni; Mannheimia haemolytica serotype 1; Pasteurella multocida; antimicrobial properties; bacteria; bovine respiratory disease; cattle; cell lines; chemical composition; cytotoxicity; essential oils; feedlots; fennel; gas chromatography-mass spectrometry; growth retardation; minimum inhibitory concentration; multiple drug resistance; nasopharynx; pathogens; serotypes; thyme
Abstract:
... AIMS: The objective of this study was to determine antimicrobial activities of essential oils (EOs) against bovine respiratory disease (BRD) pathogens and nasopharyngeal commensal bacteria, as well as cytotoxicity in bovine turbinate (BT) cells in vitro. METHODS AND RESULTS: The chemical composition of 16 EOs was determined using gas chromatography–mass spectrometry. All EOs were first evaluated f ...
DOI:
10.1111/jam.14238

15. Inhibition of Fusarium culmorum, Penicillium chrysogenum and Rhizoctonia solani by n‐hexane extracts of three plant species as a wood‐treated oil fungicide

Author:
Salem, M.Z.M.; Behiry, S.I.; EL‐Hefny, M.
Source:
Journal of applied microbiology 2019 v.126 no.6 pp. 1683-1699
ISSN:
1364-5072
Subject:
Eucalyptus camaldulensis; Fusarium culmorum; Matricaria chamomilla; Melia azedarach; Penicillium chrysogenum; Thanatephorus cucumeris; Vitex; aerial parts; air drying; antifungal properties; beta-caryophyllene; beta-sitosterol; biopesticides; cineole; flowers; food packaging; fruits; fungi; fungicides; hexane; leaves; limonene; oils; packaging materials; sabinene; transportation; vegetables; wood
Abstract:
... AIMS: Wood as a packing tool is used for packaging and transportation of fruits and vegetables for a time period varying from hours to days. During transportation, fruits and vegetables can be affected by moulds with significant postharvest problems. The present study describes the possibility of using wood‐treated oil fungicide of n‐hexane extracts from Eucalyptus camaldulensis (aerial parts), Vi ...
DOI:
10.1111/jam.14256

16. Methicillin‐sensitive and methicillin‐resistant Staphylococcus aureus strains and their toxin genes in the nostrils of dogs and workers at an animal shelter

Author:
Huang, T.‐M.; Chou, C.‐C.
Source:
Journal of applied microbiology 2019 v.126 no.6 pp. 1899-1909
ISSN:
1364-5072
Subject:
adults; animal rescue shelters; bacteria; cities; dogs; enterotoxins; genes; genotype; humans; juveniles; methicillin-resistant Staphylococcus aureus; nose; pets; risk; summer; winter; Taiwan
Abstract:
... AIMS: Methicillin‐sensitive and methicillin‐resistant Staphylococcus aureus (MSSA and MRSA, respectively) in the nostrils of dogs and workers at an animal shelter were cultured. Staphylococcal toxin genes were analysed to identify potential health concerns. METHODS AND RESULTS: Samples were obtained from 441 dogs and 9 workers. The respective isolation rates of S. aureus and MRSA were 49·0% (216/4 ...
DOI:
10.1111/jam.14266

17. Microbiome patterns reveal the transmission of pathogenic bacteria in hilsa fish (Tenualosa ilisha) marketed for human consumption in Bangladesh

Author:
Foysal, M.J.; Momtaz, F.; Robiul Kawser, A.Q.M.; Chaklader, M.R.; Siddik, M.A.B.; Lamichhane, B.; Tay, A.C.Y.; Rahman, M.M.; Fotedar, R.
Source:
Journal of applied microbiology 2019 v.126 no.6 pp. 1879-1890
ISSN:
1364-5072
Subject:
Aeromonas; Escherichia coli; Klebsiella pneumoniae; Staphylococcus aureus; Tenualosa ilisha; Vibrio cholerae; ampicillin; antibiotic resistance; bacteria; bacterial communities; disease transmission; fish; genes; high-throughput nucleotide sequencing; hindgut; humans; kanamycin; markets; microbiome; multiple drug resistance; neomycin; polymerase chain reaction; raw fish; risk; sulfamethoxazole; tetracycline; transportation; virulence; virulent strains; water pollution; waterborne diseases; Bangladesh
Abstract:
... AIMS: This study conducted bacterial community, virulence and antibiogram profiling inside the hindgut and skin of freshly caught hilsa fish and those sold at markets. METHODS AND RESULTS: The results of 16S rRNA‐based high‐throughput sequencing showed a higher number of bacterial genera in marketed fish samples than in fresh fish samples. The total operational taxonomic units, genus counts and di ...
DOI:
10.1111/jam.14257

18. Modelling of Reactive Black 5 decolourization in the presence of heavy metals by the newly isolated Pseudomonas aeruginosa strain Gb30

Author:
Louati, I.; Hadrich, B.; Nasri, M.; Belbahri, L.; Woodward, S.; Mechichi, T.
Source:
Journal of applied microbiology 2019 v.126 no.6 pp. 1761-1771
ISSN:
1364-5072
Subject:
Pseudomonas aeruginosa; bioaugmentation; cadmium; chromium; copper; decolorization; dyes; heavy metals; ions; kinetics; median effective concentration; models; textile industry; wastewater; zinc
Abstract:
... AIM: The effect of heavy metals presence on the decolourization of Reactive Black 5 by Pseudomonas aeruginosa was evaluated. METHODS AND RESULTS: In the current study, a newly isolated strain identified as P. aeruginosa strain Gb 30 was selected for its ability to remove high concentration of Reactive Black 5 and resistance to several heavy metals (Cu²⁺>Zn²⁺>Cd²⁺>Cr⁶⁺). Strain Gb30 was used to ass ...
DOI:
10.1111/jam.14262

19. Onsite advanced biocleaning system for historical wall paintings using new agar‐gauze bacteria gel

Author:
Ranalli, G.; Zanardini, E.; Rampazzi, L.; Corti, C.; Andreotti, A.; Colombini, M.P.; Bosch‐Roig, P.; Lustrato, G.; Giantomassi, C.; Zari, D.; Virilli, P.
Source:
Journal of applied microbiology 2019 v.126 no.6 pp. 1785-1796
ISSN:
1364-5072
Subject:
Fourier transform infrared spectroscopy; Pseudomonas stutzeri; agar; bacteria; biological treatment; biotechnology; cleaning; gas chromatography-mass spectrometry; gels; lipids; Vatican
Abstract:
... AIMS: This study reports the results of the application of a new agar‐gauze biogel system activated with viable bacterial cells to altered wall paintings. METHODS AND RESULTS: Biocleaning using agar biogel and agar‐gauze biogel systems was performed onsite by direct application to altered wall painting surfaces (25–1000 cm²). The treatments were performed for the restoration of two original Italia ...
DOI:
10.1111/jam.14275

20. Optimization of broth recovery for repair of heat‐injured Salmonella enterica serovar Typhimurium and Escherichia coli O157:H7

Author:
Han, J.‐Y.; Song, W.‐J.; Kang, D.‐H.
Source:
Journal of applied microbiology 2019 v.126 no.6 pp. 1923-1930
ISSN:
1364-5072
Subject:
Escherichia coli O157; Salmonella Typhimurium; agar; cell suspension culture; liquids; microorganisms; milk; pathogens; phenolsulfonphthalein; selective media; sorbitol; yeast extract
Abstract:
... AIMS: The purpose of this research was to determine optimum conditions for broth recovery of heat‐injured Salmonella Typhimurium and Escherichia coli O157:H7. METHODS AND RESULTS: Exposure to 55°C for 15 and 25 min, respectively, induced cellular injury to those pathogens. Comparison was made with the commonly used overlay method using selective medium for recovering sublethally injured cells of S ...
DOI:
10.1111/jam.14263