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You searched for: Journal name Journal of applied microbiology Remove constraint Journal name: Journal of applied microbiology Publication year rev 7981-2019 Remove constraint Publication year rev: 7981-2019 Subject term minimum inhibitory concentration Remove constraint Subject term: minimum inhibitory concentration Text availability Citation in PubAg Remove constraint Text availability: Citation in PubAg
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1. A new acridone with antifungal properties against Candida spp. and dermatophytes, and antibiofilm activity against C. albicans

Author:
D.B.C. de Oliveira; L.B. Silva; B.V. da Silva; T.C. Borges; B.C. Marques; M.B. dos Santos; L.F. de Oliveira; V.S. Bolzani; A.R.A. Rodrigues; L.O. Regasini; A.A. Andrade
Source:
Journal of applied microbiology 2019 v.127 no.5 pp. 1362-1372
ISSN:
1364-5072
Subject:
Candida albicans; Trichophyton rubrum; antifungal agents; antifungal properties; biofilm; confocal laser scanning microscopy; cytotoxicity; drug therapy; fibroblasts; hyphae; keratinophilic fungi; minimum inhibitory concentration; viability
Abstract:
... AIM: The increase in the number of fungal infections worldwide, coupled with the limitations of current antifungal chemotherapy, demand the development of safe and effective new antifungals. Here, we presented the synthesis of a novel acridone (M14) and its antifungal properties against Candida and dermatophytes species. METHODS AND RESULTS: A series of 17 acridones was designed, synthesized and t ...
DOI:
10.1111/jam.14381

2. A review on biocide reduced susceptibility due to plasmid‐borne antiseptic‐resistant genes—special notes on pharmaceutical environmental isolates

Author:
R. Vijayakumar; T. Sandle
Source:
Journal of applied microbiology 2019 v.126 no.4 pp. 1011-1022
ISSN:
1364-5072
Subject:
antibiotic resistance; antiseptics; biocides; disinfectants; genes; hospitals; issues and policy; microorganisms; minimum inhibitory concentration; pharmaceutical industry
Abstract:
... Biocides (antiseptics and disinfectants) are widely used in hospitals and pharmaceutical industries for contamination control. The emergence of reduced susceptibility to biocides is the major concern and this is caused by various factors, among which plasmid‐mediated resistance is common. Many publications describe the antibiotic resistance and mechanisms in a clinical setting. However, there are ...
DOI:
10.1111/jam.14118

3. Antibiotic‐resistant Staphylococcus aureus strains of swine origin: molecular typing and susceptibility to oregano (Origanum vulgare L.) essential oil and maqui (Aristotelia chilensis (Molina) Stuntz) extract

Author:
A. Lofa; V. Velasco; M. Gerding; M.D. López; D. Vallejos; A.M. Bonilla; C.M. Logue
Source:
Journal of applied microbiology 2019 v.127 no.4 pp. 1048-1056
ISSN:
1364-5072
Subject:
Aristotelia chilensis; Origanum vulgare; Staphylococcus aureus; antibiotic resistance; antibiotics; blackberries; carvacrol; disk diffusion antimicrobial test; essential oils; minimum inhibitory concentration; multiple drug resistance; oregano; pork; supply chain; swine; thymol
Abstract:
... AIM: The molecular typing and the susceptibility of Staphylococcus aureus strains of swine origin to antibiotics, oregano (Origanum vulgare L.) essential oil (EO) and Chilean blackberry maqui (Aristotelia chilensis (Molina) Stuntz) extract were determined. METHODS AND RESULTS: Twenty S. aureus strains of swine origin were subjected to molecular typing, of which six strains were selected for antimi ...
DOI:
10.1111/jam.14393

4. Antimicrobial effects of cinnamon essential oil and cinnamaldehyde combined with EDTA against canine otitis externa pathogens

Author:
J.X.F. Sim; M. Khazandi; H. Pi; H. Venter; D.J. Trott; P. Deo
Source:
Journal of applied microbiology 2019 v.127 no.1 pp. 99-108
ISSN:
1364-5072
Subject:
EDTA (chelating agent); Gram-negative bacteria; Malassezia pachydermatis; Pseudomonas aeruginosa; anti-infective agents; antibacterial properties; antibiotic resistance; bark; cinnamon; cinnamon oil; dogs; fungi; minimum inhibitory concentration; oils; otitis externa; pathogens; synergism
Abstract:
... AIMS: The antimicrobial activity of cinnamon essential oil and cinnamaldehyde against bacterial and fungal pathogens associated with canine otitis externa, as well as the effect of their combination with EDTA were investigated. METHODS AND RESULTS: Antimicrobial susceptibility testing was performed using the broth microdilution method while spot‐plating technique was used to determine their bacter ...
DOI:
10.1111/jam.14298

5. Association of Myroides odoratimimus in immunocompromized piglets with post weaning multisystemic wasting syndrome

Author:
M. Choudhary; B.K. Choudhary; B.C. Bera; S.P. Chaudhari; D.K. Giri; R.C. Ghosh; S.B. Barbuddhe
Source:
Journal of applied microbiology 2019 v.127 no.6 pp. 1635-1645
ISSN:
1364-5072
Subject:
Myroides; Porcine circovirus-2; aminoglycosides; animal pathogens; antibiotic resistance; carbapenems; cephalosporins; disease severity; fluoroquinolones; health hazards; immunocompromised population; immunosuppression; matrix-assisted laser desorption-ionization mass spectrometry; minimum inhibitory concentration; mortality; multiple drug resistance; pathogen identification; phenotype; phylogeny; piglets; pneumonia; public health; secondary infection; wasting syndrome; weaning
Abstract:
... AIM: To study the association of opportunistic infection due to Myroides odoratimimus in piglets immunocompromised by porcine circovirus type 2 (PCV2) infection. METHODS AND RESULTS: The clinical samples (n = 101) were analysed bacteriologically. The isolates were identified by their phenotypes and MALDI TOF‐MS analysis as Myroides species. The phylogram constructed based on nucleotide sequences o ...
DOI:
10.1111/jam.14448

6. Bactericidal and cytotoxic activity of a diarylheptanoid (etlingerin) isolated from a ginger (Etlingera pubescens) endemic to Borneo

Author:
D. Daniel‐Jambun; K.S. Ong; Y.Y. Lim; J.B.L. Tan; S.W. Yap; S.M. Lee
Source:
Journal of applied microbiology 2019 v.127 no.1 pp. 59-67
ISSN:
1364-5072
Subject:
Bacillus subtilis; Etlingera pubescens; Gram-positive bacteria; Staphylococcus aureus; acetates; animal models; anti-infective agents; antimicrobial properties; bacterial infections; cell lines; chemical structure; curcumin; cytotoxicity; ginger; humans; inhibitory concentration 50; membrane permeability; methanol; minimum inhibitory concentration; Borneo
Abstract:
... AIMS: The aim of this study was to investigate the antimicrobial activities of Etlingera pubescens, and to isolate and identify the antimicrobial compound. METHODS AND RESULTS: The crude extracts of E. pubescens were obtained through methanol extraction, and evaluated for antimicrobial activities. From this extract, 1,7‐bis(3,4‐dihydroxyphenyl)heptan‐3‐yl acetate (etlingerin) was isolated. When co ...
DOI:
10.1111/jam.14287

7. Biofilm inhibiting activity of betacyanins from red pitahaya (Hylocereus polyrhizus) and red spinach (Amaranthus dubius) against Staphylococcus aureus and Pseudomonas aeruginosa biofilms

Author:
Y.Y. Yong; G. Dykes; S.M. Lee; W.S. Choo
Source:
Journal of applied microbiology 2019 v.126 no.1 pp. 68-78
ISSN:
1364-5072
Subject:
Amaranthus dubius; Hylocereus polyrhizus; Pseudomonas aeruginosa; Staphylococcus aureus; antimicrobial properties; betacyanins; biofilm; cell viability; hydrophobicity; leaves; methanol; minimum inhibitory concentration; polystyrenes; pulp; risk reduction; spinach
Abstract:
... AIMS: To investigate the biofilm inhibitory activity of betacyanins from red pitahaya (Hylocereus polyrhizus) and red spinach (Amaranthus dubius) against Staphylococcus aureus and Pseudomonas aeruginosa biofilms. METHODS AND RESULTS: The pulp of red pitahaya and the leaves of red spinach were extracted using methanol followed by subfractionation to obtain betacyanin fraction. The anti‐biofilm acti ...
DOI:
10.1111/jam.14091

8. Biological control of Pseudomonas syringae pv. aptata on sugar beet with Bacillus pumilus SS‐10.7 and Bacillus amyloliquefaciens (SS‐12.6 and SS‐38.4) strains

Author:
I. Nikolić; T. Berić; I. Dimkić; T. Popović; J. Lozo; D. Fira; S. Stanković
Source:
Journal of applied microbiology 2019 v.126 no.1 pp. 165-176
ISSN:
1364-5072
Subject:
Bacillus amyloliquefaciens; Bacillus pumilus; Pseudomonas syringae pv. aptata; antibacterial properties; biological control; cell culture; disease control; disease severity; growers; leaf spot; lipopeptides; minimum inhibitory concentration; necrosis; pathogens; sugar beet
Abstract:
... AIM: Assessment of biological control of Pseudomonas syringae pv. aptata using crude lipopeptide extracts (CLEs) of two Bacillus amyloliquefaciens strains (SS‐12.6 and SS‐38.4) and one Bacillus pumilus strain (SS‐10.7). METHODS AND RESULTS: The minimum inhibitory concentration (MIC) of CLEs and their combinations against the pathogen and potential interaction between the extracts were determined i ...
DOI:
10.1111/jam.14070

9. Characterization of maltocin S16, a phage tail‐like bacteriocin with antibacterial activity against Stenotrophomonas maltophilia and Escherichia coli

Author:
J. Chen; Y. Zhu; M. Yin; Y. Xu; X. Liang; Y.‐P. Huang
Source:
Journal of applied microbiology 2019 v.127 no.1 pp. 78-87
ISSN:
1364-5072
Subject:
Escherichia coli; Stenotrophomonas maltophilia; adsorption; antibacterial properties; antibiotics; bacteriocins; bacteriophages; infectious diseases; lipopolysaccharides; minimum inhibitory concentration; morbidity; mortality; multigene family; multiple drug resistance; open reading frames; peptidase K; phylogeny; secondary infection; trypsin
Abstract:
... AIMS: To determine the characteristics of the novel phage tail‐like bacteriocin (PTLB) produced by Stenotrophomonas maltophilia S16. METHODS AND RESULTS: A screen to identify novel bacteriocins from a panel of 86 S. maltophilia strains was performed. We found that 62 tested S. maltophilia strains were sensitive to a PTLB, designated maltocin S16. In addition, 8 of 14 tested Escherichia coli strain ...
DOI:
10.1111/jam.14294

10. Detection of antibiotic susceptibility by colorimetric minimum inhibitory concentration in staphylococcal isolates

Author:
B.S. Mahmoud; S.A. ElMasry; N.A.E.M.M. Fahim; M.A. Abd ElSattar; O.A. Shaker
Source:
Journal of applied microbiology 2019 v.127 no.3 pp. 693-700
ISSN:
1364-5072
Subject:
agar; antibiotic resistance; biphenyl; blood flow; ciprofloxacin; clindamycin; colorimetry; erythromycin; gentamicin; hospitals; linezolid; methicillin; methicillin-resistant Staphylococcus aureus; minimum inhibitory concentration; mortality; oxacillin; patients; pneumonia; tetracycline; tetrazolium; thiazoles; vancomycin
Abstract:
... AIM: Assess performance of broth microdilution (BMD) as well as agar dilution methods for antimicrobial susceptibility testing of Staphylococci using tetrazolium salt. METHODS AND RESULTS: Minimum inhibitory concentration (MIC) of eight antimicrobials; vancomycin (VA), linezolid, oxacillin, gentamicin (CN), tetracycline, ciprofloxacin, erythromycin and clindamycin was investigated for 80 isolates ...
DOI:
10.1111/jam.14347

11. Detection of extended‐spectrum β‐lactam, AmpC and carbapenem resistance in Enterobacteriaceae in beef cattle in Great Britain in 2015

Author:
M. Velasova; R.P. Smith; F. Lemma; R.A. Horton; N.A. Duggett; J. Evans; S.C. Tongue; M.F. Anjum; L.P. Randall
Source:
Journal of applied microbiology 2019 v.126 no.4 pp. 1081-1095
ISSN:
1364-5072
Subject:
Citrobacter; Escherichia coli; Serratia; beef cattle; beta-lactamase; beta-lactamase bacteria; bulls; carbapenems; farms; feces; genetic variation; herds; matrix-assisted laser desorption-ionization mass spectrometry; minimum inhibitory concentration; phenotype; polymerase chain reaction; pulsed-field gel electrophoresis; purchasing; resistance genes; risk factors; sequence analysis; England; Scotland
Abstract:
... AIMS: This study investigated the occurrence and genetic diversity of Enterobacteriaceae with extended‐spectrum β‐lactamase (ESBL)‐, AmpC‐ and carbapenemase‐mediated resistance in British beef cattle, and related risk factors. METHODS AND RESULTS: Faecal samples (n = 776) were obtained from farms in England and Wales (n = 20) and Scotland (n = 20) in 2015. Isolates from selective agars were identi ...
DOI:
10.1111/jam.14211

12. Enterocin LD3 from Enterococcus hirae LD3 causing efflux of intracellular ions and UV‐absorbing materials in Gram‐negative bacteria

Author:
Poonam Sheoran; Santosh Kumar Tiwari
Source:
Journal of applied microbiology 2019 v.126 no.4 pp. 1059-1069
ISSN:
1364-5072
Subject:
Enterococcus hirae; Escherichia coli; Gram-negative bacteria; Micrococcus luteus; absorbance; antibacterial properties; bacteriocins; cell membranes; electrical conductivity; gel electrophoresis; ions; mechanism of action; minimum inhibitory concentration; nucleic acids; potassium; transmission electron microscopy
Abstract:
... AIMS: To understand the bactericidal action of enterocin LD3 against Gram‐negative bacteria. METHODS AND RESULTS: Minimum inhibitory concentration (MIC) of enterocin LD3 against Micrococcus luteus MTCC 106 and Escherichia coli NCDC 135 was 80 and 112 μg ml⁻¹, and minimum bactericidal concentration (MBC) was 128 and 180 μg ml⁻¹, respectively. The efflux of potassium ion (K⁺) was 14 and 13 ppm and e ...
DOI:
10.1111/jam.14203

13. Essential oils inhibit the bovine respiratory pathogens Mannheimia haemolytica, Pasteurella multocida and Histophilus somni and have limited effects on commensal bacteria and turbinate cells in vitro

Author:
S. Amat; D. Baines; E. Timsit; J. Hallewell; T.W. Alexander
Source:
Journal of applied microbiology 2019 v.126 no.6 pp. 1668-1682
ISSN:
1364-5072
Subject:
Haemophilus somni; Mannheimia haemolytica serotype 1; Pasteurella multocida; antimicrobial properties; bacteria; bovine respiratory disease; cattle; cell lines; chemical composition; cytotoxicity; essential oils; feedlots; fennel; gas chromatography-mass spectrometry; growth retardation; minimum inhibitory concentration; multiple drug resistance; nasopharynx; pathogens; serotypes; thyme
Abstract:
... AIMS: The objective of this study was to determine antimicrobial activities of essential oils (EOs) against bovine respiratory disease (BRD) pathogens and nasopharyngeal commensal bacteria, as well as cytotoxicity in bovine turbinate (BT) cells in vitro. METHODS AND RESULTS: The chemical composition of 16 EOs was determined using gas chromatography–mass spectrometry. All EOs were first evaluated f ...
DOI:
10.1111/jam.14238

14. Evaluating the antibacterial and antibiofilm potential of sulphated polysaccharides extracted from green algae Chlamydomonas reinhardtii

Author:
J. Vishwakarma; S.L. Vavilala
Source:
Journal of applied microbiology 2019 v.127 no.4 pp. 1004-1017
ISSN:
1364-5072
Subject:
Bacillus subtilis; Chlamydomonas reinhardtii; DNA; Escherichia coli; Neisseria mucosa; Streptococcus; antibacterial properties; antibiotics; bacteria; biochemical pathways; biofilm; dose response; minimum inhibitory concentration; polymers; polysaccharides
Abstract:
... AIM OF THE STUDY: In this study, antibacterial and antibiofilm potential of sulphated polysaccharides (SPs) extracted from Chlamydomonas reinhardtii (Cr) was evaluated against Neisseria mucosa, Escherichia coli, Streptococcus sp. and Bacillus subtilis. METHODS AND RESULTS: Antibacterial potential of Cr‐SPs was evaluated by agar‐cup diffusion, time‐kill and colony‐forming ability (CFU), minimum inh ...
DOI:
10.1111/jam.14364

15. Growth optimization, free radical scavenging and antibacterial potential of Chlorella sp. SRD3 extracts against clinical isolates

Author:
S. Santhosh; N. Manivannan; C. Ragavendran; N. Mathivanan; D. Natarajan; N. Hemalatha; R. Dhandapani
Source:
Journal of applied microbiology 2019 v.127 no.2 pp. 481-494
ISSN:
1364-5072
Subject:
Chlorella; Gram-negative bacteria; antibacterial properties; antioxidant activity; biomass production; cost effectiveness; culture media; erythrocytes; ethyl acetate; flavonoids; fluorescent lighting; food industry; free radical scavengers; gas chromatography-mass spectrometry; hemolysis; hexane; methanol; microalgae; microscopy; minimum inhibitory concentration; pathogens; proanthocyanidins; quantitative analysis; screening; solar radiation
Abstract:
... AIM: The aim of present work was to explore the potential of Chlorella sp. SRD3 extracts for antioxidant and antibacterial activity along with the evaluation of minimum inhibitory concentration (MIC) and haemolytic activity to detect RBC cell damage. METHODS AND RESULTS: Screening and isolation of microalgae was performed using bold basal medium under normal illuminance (at 27°C) and microscopic o ...
DOI:
10.1111/jam.14336

16. In vitro potentiation of carbapenems with tannic acid against carbapenemase‐producing enterobacteriaceae: exploring natural products as potential carbapenemase inhibitors

Author:
A.M. Somboro; J. Osei Sekyere; D.G. Amoako; H.M. Kumalo; R. Khan; L.A. Bester; S.Y. Essack
Source:
Journal of applied microbiology 2019 v.126 no.2 pp. 452-467
ISSN:
1364-5072
Subject:
Enterobacteriaceae; active sites; adjuvants; beta-lactamase; binding capacity; carbapenems; cytotoxicity; enzyme inhibition; enzyme inhibitors; hydrophobicity; inhibitory concentration 50; minimum inhibitory concentration; tannins; transporters
Abstract:
... AIMS: We hypothesized and confirmed that tannic acid (TA) reverses carbapenem resistance by inhibiting carbapenemases in class A and B carbapenemase‐producing Enterobacteriaceae. METHODS AND RESULTS: Minimum inhibitory concentrations of carbapenems in the presence and absence of TA and other efflux pump inhibitors, TA‐carbapenemases inhibition assays and computational studies showed that TA had th ...
DOI:
10.1111/jam.14141

17. Lactocin AL705 as quorum sensing inhibitor to control Listeria monocytogenes biofilm formation

Author:
C. Melian; F. Segli; R. Gonzalez; G. Vignolo; P. Castellano
Source:
Journal of applied microbiology 2019 v.127 no.3 pp. 911-920
ISSN:
1364-5072
Subject:
Listeria monocytogenes; Vibrio harveyi; antibacterial properties; bacteriocins; biofilm; food contamination; luminescence; microorganisms; minimum inhibitory concentration; pathogens; quorum sensing; sanitation; screening
Abstract:
... AIMS: The control of Listeria monocytogenes biofilm formation using lactocin AL705 bacteriocin at sub‐minimum inhibitory concentrations (MICs) through an antiquorum sensing strategy, was preliminarily investigated. METHODS AND RESULTS: The screening for biofilm formation of different Listeria species at 10°C allowed selecting L. monocytogenes FBUNT for its use as biofilm producer. MIC and minimum ...
DOI:
10.1111/jam.14348

18. Mechanism for antibacterial action of epigallocatechin gallate and theaflavin‐3,3′‐digallate on Clostridium perfringens

Author:
T. Noor Mohammadi; A.T. Maung; J. Sato; T. Sonoda; Y. Masuda; K. Honjoh; T. Miyamoto
Source:
Journal of applied microbiology 2019 v.126 no.2 pp. 633-640
ISSN:
1364-5072
Subject:
Clostridium perfringens; DNA; antibacterial properties; bacteria; cell division; cell growth; epigallocatechin gallate; fluorescence microscopy; food additives; foodborne illness; minimum inhibitory concentration; people; poisoning; polyphenols; proteins; tea; washing
Abstract:
... AIM: The purpose of this study was to clarify the mechanism of the antibacterial action of two high potential and natural food additives, epigallocatechin gallate (EGCg) and theaflavin‐3,3′‐digallate (TF3), on Clostridium perfringens. METHODS AND RESULTS: Minimal inhibitory concentrations were determined by the serial dilution method. Afterwards, the cells were treated with 250 or 1000 mg l⁻¹ of E ...
DOI:
10.1111/jam.14134

19. Natural products show diverse mechanisms of action against Clostridium difficile

Author:
N. Roshan; T.V. Riley; D.R. Knight; J.H. Steer; K.A. Hammer
Source:
Journal of applied microbiology 2019 v.126 no.2 pp. 468-479
ISSN:
1364-5072
Subject:
Clostridium difficile; Escherichia coli; Leptospermum scoparium; absorbance; adenosine triphosphate; allicin; antibacterial properties; antibiotic resistance; bulbs; cell membranes; cell suspension culture; cinnamon; garlic; mechanism of action; menthol; minimum inhibitory concentration; peppermint oil; propidium; protein synthesis
Abstract:
... AIMS: To investigate the mechanisms of action of natural products with bactericidal (cinnamon root powder, peppermint oil, trans‐cinnamaldehyde, menthol and zingerone) or bacteriostatic (fresh garlic bulb extract, garlic clove powder, Leptospermum honey and allicin) activity against two Clostridium difficile strains. METHODS AND RESULTS: Bactericidal products significantly reduced intracellular AT ...
DOI:
10.1111/jam.14152

20. Quercetin potentiates meropenem activity among pathogenic carbapenem‐resistant Pseudomonas aeruginosa and Acinetobacter baumannii

Author:
A. Pal; A. Tripathi
Source:
Journal of applied microbiology 2019 v.127 no.4 pp. 1038-1047
ISSN:
1364-5072
Subject:
Acinetobacter baumannii; Pseudomonas aeruginosa; antibacterial properties; cell walls; meropenem; minimum inhibitory concentration; quantitative polymerase chain reaction; quercetin; reverse transcriptase polymerase chain reaction; synergism
Abstract:
... AIMS: Global spread of carbapenem‐resistant Pseudomonas aeruginosa (CRPsA) and Acinetobacter baumannii (CRAB) is an emerging clinical problem. Since, quercetin is a well‐known antibacterial agent; it would be relevant to demonstrate synergy between quercetin and meropenem and elucidate molecular basis of effective bactericidal activity of quercetin‐meropenem against CRPsA and CRAB. METHODS AND RES ...
DOI:
10.1111/jam.14388