Jump to Main Content
- Lee, C.S.; Wetzel, K.; Buckley, T.; Wozniak, D.; Lee, J.
- Journal of applied microbiology 2011 v.111 no.4 pp. 893-903
- DNA; DNA primers; Legionella pneumophila; Pseudomonas aeruginosa; aerosols; airborne transmission; bacteria; burden of disease; chlorine; cystic fibrosis; detection limit; drinking water; filters; gelatin; genes; neutralization; patients; quantitative polymerase chain reaction; risk assessment
- ... Aims: For the rapid detection of Pseudomonas aeruginosa from chlorinated water and aerosols, gyrB geneâbased realâtime PCR assay was developed and investigated. Methods and Results: Two novel primer sets (pa722F/746MGB/899R and pa722F/746MGB/788R) were designed using the most updated 611 Pseudomonas and 748 other bacterial gyrB genes for achieving high specificity. Their specificity showed 100 ...
- PubMed Central: